BMRBj - BMREntryMaster

Found 1 Document (1.249 seconds)

BMRB: 5825

1Q6A

Sequence-specific 1H, 13C and 15N resonance assignments of the C-terminal domain of KaiA, a circadian clock protein (reduced form)

Authors

Vakonakis, I., LiWang, A.C.

Assembly

KaiA C-terminal domain

Entity

1. KaiA C-terminal domain (polymer, Thiol state: all free), 107 monomers, 12583.44 × 2 Da Detail

AMARMSPADK RKLLDELRSI YRTIVLEYFN TDAKVNERID EFVSKAFFAD ISVSQVLEIH VELMDTFSKQ LKLEGRSEDI LLDYRLTLID VIAHLCEMYR RSIPREV

Total weight

25166.88 Da

Max. entity weight

12583.44 Da

Source organism

Thermosynechococcus elongatus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.5 %, Completeness: 29.4 %, Completeness (bb): 58.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	29.4 % (381 of 1297)	15.6 % (105 of 675)	35.5 % (182 of 513)	86.2 % (94 of 109)
Backbone	58.3 % (372 of 638)	46.5 % (99 of 213)	55.9 % (179 of 320)	89.5 % (94 of 105)
Sidechain	12.3 % (94 of 765)	1.3 % (6 of 462)	29.4 % (88 of 299)	0.0 % (0 of 4)
Aromatic	0.0 % (0 of 90)	0.0 % (0 of 45)	0.0 % (0 of 45)
Methyl	8.5 % (12 of 142)	5.6 % (4 of 71)	11.3 % (8 of 71)

1. Thermosynechococcus KaiA

AMARMSPADK RKLLDELRSI YRTIVLEYFN TDAKVNERID EFVSKAFFAD ISVSQVLEIH VELMDTFSKQ LKLEGRSEDI LLDYRLTLID VIAHLCEMYR RSIPREV

Show sample condition

Sample

Temperature 323 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Thermosynechococcus KaiA	[U-99% 13C; U-99% 15N]	1.2 mM
2	Na2HPO4		20.0 mM
3	NaCl		20.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.3 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.3 ppm, Outliers: 1 Detail

Release date

2004-02-12

Citation

Sequence-specific 1H, 13C and 15N resonance assignments of the C-terminal domain of KaiA, a circadian clock protein
Vakonakis, I., LiWang, A.C.
J. Biomol. NMR (2004), 28, 403-404, PubMed 14872133 , DOI 10.1023/B:JNMR.0000015373.13794.c7 , Abstract

Related entities 1. KaiA C-terminal domain, : 1 : 3 : 4 : 13 entities Detail

Interaction partners 1. KaiA C-terminal domain, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5825_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5825_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	OK	367		93.5 (chain: 1, length: 107)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 107, Sequence coverage: 93.5%
- Total number of assignments
  - ¹³C chemical shifts: 177
  - ¹H chemical shifts: 95
  - ¹⁵N chemical shifts: 95
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	513	177	34.5
¹H chemical shifts	675	95	14.1
¹⁵N chemical shifts	119	95	79.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	214	92	43.0
¹H chemical shifts	213	95	44.6
¹⁵N chemical shifts	105	95	90.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	299	85	28.4
¹H chemical shifts	462	0	0.0
¹⁵N chemical shifts	14	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	75	5	6.7
¹H chemical shifts	75	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	45	0	0.0
¹H chemical shifts	45	0	0.0

Keywords assignment, circadian clock, KaiA, Thermosynechococcus elongatus

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Found 1 Document (1.249 seconds)

BMRB: 5825

Sample

Chemical shift reference

Chemical shift reference

Related entities 1. KaiA C-terminal domain, : 1 : 3 : 4 : 13 entities Detail

Interaction partners 1. KaiA C-terminal domain, : 1 interactors Detail

Experiments performed 2 experiments Detail

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail