BMRB: 5843

Solution Structure of At3g17210

Authors

Volkman, B.F.

Assembly

expressed protein: At3g17210.1

Entity

1. expressed protein: At3g17210.1 (polymer, Thiol state: not present), 112 monomers, 12465.09 × 2 Da Detail

GSHMEEAKGP VKHVLLASFK DGVSPEKIEE LIKGYANLVN LIEPMKAFHW GKDVSIENLH QGYTHIFEST FESKEAVAEY IAHPAHVEFA TIFLGSLDKV LVIDYKPTSV SL

Total weight

24930.18 Da

Max. entity weight

12465.09 Da

Source organism

Arabidopsis thaliana

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.5 %, Completeness (bb): 98.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.5 % (1245 of 1318)	94.7 % (641 of 677)	93.4 % (494 of 529)	98.2 % (110 of 112)
Backbone	98.3 % (651 of 662)	99.6 % (225 of 226)	97.6 % (321 of 329)	98.1 % (105 of 107)
Sidechain	91.9 % (699 of 761)	92.2 % (416 of 451)	91.1 % (278 of 305)	100.0 % (5 of 5)
Aromatic	81.1 % (107 of 132)	95.5 % (63 of 66)	66.2 % (43 of 65)	100.0 % (1 of 1)
Methyl	98.6 % (136 of 138)	100.0 % (69 of 69)	97.1 % (67 of 69)

1. expressed protein At3g17210.1

GSHMEEAKGP VKHVLLASFK DGVSPEKIEE LIKGYANLVN LIEPMKAFHW GKDVSIENLH QGYTHIFEST FESKEAVAEY IAHPAHVEFA TIFLGSLDKV LVIDYKPTSV SL

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	expressed protein At3g17210.1	[U-13C; U-15N]		1 mM
2	sodium phosphate			20 mM
3	sodium chloride			50 mM
4	H2O			90 %
5	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.72 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.72 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 1Q53, Strand ID: A, B Detail

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Release date

2003-07-16

Citation

Letter to the Editor: Structure of the hypothetical protein At3g17210 from Arabidopsis thaliana
Lytle, B.L., Peterson, F.C., Kjer, K.L., Frederick, R.O., Zhao, Q., Thao, S., Bingman, C., Johnson, K.A., Phillips, G.N., Volkman, B.F.
J. Biomol. NMR (2004), 28, 397-400, PubMed , DOI:

Related entities 1. expressed protein: At3g17210.1, : 1 : 4 : 49 entities Detail

Interaction partners 1. expressed protein: At3g17210.1, : 52 interactors Detail

Experiments performed 12 experiments Detail