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BMRB: 5854


5854
1FYD
1H, 13C, and 15N backbone assignments for the 60.8 kD dimer of the NAD+ synthetase from Bacillus subtilis
Authors
Markus, M.A., Doliveira, L., Malakian, K., Keeney, D., Severin, A., Underwood, K.W., Tsao, D.H. H.
Assembly
NAD+ synthetase
Entity
1. NAD+ synthetase (polymer, Thiol state: not present), 272 monomers, 30394.82 × 2 Da Detail

MSMQEKIMRE LHVKPSIDPK QEIEDRVNFL KQYVKKTGAK GFVLGISGGQ DSTLAGRLAQ LAVESIREEG GDAQFIAVRL PHGTQQDEDD AQLALKFIKP DKSWKFDIKS TVSAFSDQYQ QETGDQLTDF NKGNVKARTR MIAQYAIGGQ EGLLVLGTDH AAEAVTGFFT KYGDGGADLL PLTGLTKRQG RTLLKELGAP ERLYLKEPTA DLLDEKPQQS DETELGISYD EIDDYLEGKE VSAKVSEALE KRYSMTEHKR QVPASMFDDW WK


Total weight
60789.64 Da
Max. entity weight
30394.82 Da
Source organism
Bacillus subtilis
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.8 %, Completeness: 45.1 %, Completeness (bb): 81.0 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All45.1 % (1431 of 3170)23.2 % (384 of 1658)64.9 % (794 of 1224)87.8 % (253 of 288)
Backbone81.0 % (1308 of 1614)52.1 % (291 of 559)96.5 % (764 of 792)96.2 % (253 of 263)
Sidechain20.0 % (361 of 1804) 8.6 % (94 of 1099)39.3 % (267 of 680) 0.0 % (0 of 25)
Aromatic 2.8 % (6 of 216) 5.6 % (6 of 108) 0.0 % (0 of 105) 0.0 % (0 of 3)
Methyl18.4 % (52 of 282)14.2 % (20 of 141)22.7 % (32 of 141)

1. NAD+ synthetase

MSMQEKIMRE LHVKPSIDPK QEIEDRVNFL KQYVKKTGAK GFVLGISGGQ DSTLAGRLAQ LAVESIREEG GDAQFIAVRL PHGTQQDEDD AQLALKFIKP DKSWKFDIKS TVSAFSDQYQ QETGDQLTDF NKGNVKARTR MIAQYAIGGQ EGLLVLGTDH AAEAVTGFFT KYGDGGADLL PLTGLTKRQG RTLLKELGAP ERLYLKEPTA DLLDEKPQQS DETELGISYD EIDDYLEGKE VSAKVSEALE KRYSMTEHKR QVPASMFDDW WK

Show sample condition
Sample

Temperature 308 (±0.2) K, pH 7.5 (±0.1)


#NameIsotope labelingTypeConcentration
1NAD+ synthetase[U-2H; U-13C; U-15N]1.0 mM

LACS Plot; CA
Referencing offset: 0.5 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: 0.5 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: 0.03 ppm, Outliers: 2 Detail
Release date
2004-02-09
Citation 1
1H, 13C, and 15N backbone assignments and secondary structure for the 60.8 kD dimer of the NAD+ synthetase from Bacillus subtilis
Markus, M.A., Doliveira, L., Malakian, K., Keeney, D., Severin, A., Underwood, K.W., Tsao, D.H.H.
J. Biomol. NMR (2004), 28, 301-302, PubMed 14752265 , DOI 10.1023/B:JNMR.0000013682.41230.8b ,
Show more 2 citaions
Citation 2
NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 ,
Citation 3
A short recollection on the paper entitled "A common sense approach to peak picking in two-, three-, and four-dimensional spectra using automatic computer analysis of contour diagrams" by D.S. Garrett, R. Powers, A.M. Gronenborn, and G.M. Clore [J. Magn. Reson. 95 (1991) 214-220]
J. Magn. Reson. (2011), 213, 364-365, PubMed 22152356 , DOI 10.1016/j.jmr.2011.08.009 ,
Related entities 1. NAD+ synthetase, : 1 : 1 : 6 : 139 entities Detail
More details for 147 related entities
Experiments performed 2 experiments Detail
Chemical shift validation 3 contents Detail
Keywords antibiotic target, NAD synthesis pathway, resonance assignments, TROSY