NMR assignment of human Sip(7-50)
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 66.2 % (408 of 616) | 57.8 % (190 of 329) | 75.4 % (175 of 232) | 78.2 % (43 of 55) |
Backbone | 78.1 % (239 of 306) | 79.6 % (82 of 103) | 75.0 % (114 of 152) | 84.3 % (43 of 51) |
Sidechain | 57.5 % (207 of 360) | 47.8 % (108 of 226) | 76.2 % (99 of 130) | 0.0 % (0 of 4) |
Aromatic | 0.0 % (0 of 4) | 0.0 % (0 of 2) | 0.0 % (0 of 2) | |
Methyl | 60.7 % (34 of 56) | 64.3 % (18 of 28) | 57.1 % (16 of 28) |
1. GSH-SIP(short)
GSHMAASEEL QKDLEEVKVL LEKATRKRVR DALTAEKSKI ETEIKNKMQQ KTemperature 303 (±0.3) K, pH 5.1 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | GSH-SIP(short) | [U-15N; U-13C] | 1.0 mM |