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BMRB: 5858

2GIM

Characterization of us-ms Dynamics of Proteins Using a Combined Analysis of 15N NMR Relaxation and Chemical Shift: Conformational Exchange in Plastocyanin Induced by Histidine Protonations

Authors

Hass, M.A.S., Thuesen, M.H., Christensen, H.E.M., Led, J.J.

Assembly

Cu (I) Plastocyanin from Anabaena Variabilis

Entity

1. Cu(II) Plastocyanin from Anabeana Variabilis (polymer, Thiol state: all other bound), 106 monomers, 11234.73 Da Detail

METYTVKLGS DKGLLVFEPA KLTIKPGDTV EFLNNKVPPH NVVFDAALNP AKSADLAKSL SHKQLLMSPG QSTSTTFPAD APAGEYTFYC EPHRGAGMVG KITVAG

2. CU1 (non-polymer), 63.546 Da

Total weight

11298.276 Da

Max. entity weight

11234.73 Da

Entity Connection

na 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS90:SG	2:CU11:CU

Source organism

Trichormus variabilis

Exptl. method

NMR

Data set

assigned_chemical_shifts, heteronucl_T1_relaxation, heteronucl_T2_relaxation

Chem. Shift Complete1

Sequence coverage: 87.7 %, Completeness: 40.1 %, Completeness (bb): 66.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	40.1 % (287 of 715)	32.2 % (197 of 612)	87.4 % (90 of 103)
Backbone	66.0 % (204 of 309)	53.8 % (114 of 212)	92.8 % (90 of 97)
Sidechain	20.4 % (83 of 406)	20.7 % (83 of 400)	0.0 % (0 of 6)
Aromatic	11.6 % (5 of 43)	11.6 % (5 of 43)
Methyl	30.0 % (18 of 60)	30.0 % (18 of 60)

1. Cu(II) Plastocyanin from Anabeana Variabilis

METYTVKLGS DKGLLVFEPA KLTIKPGDTV EFLNNKVPPH NVVFDAALNP AKSADLAKSL SHKQLLMSPG QSTSTTFPAD APAGEYTFYC EPHRGAGMVG KITVAG

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 8.50 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Cu(II) Plastocyanin from Anabeana Variabilis	[U-95% 15N]		1.0 mM
2	natrium ascorbate			0.1 mM

Chem. Shift Complete2

Sequence coverage: 90.6 %, Completeness: 41.3 %, Completeness (bb): 68.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	41.3 % (591 of 1430)	33.2 % (406 of 1224)	89.8 % (185 of 206)
Backbone	68.4 % (423 of 618)	56.4 % (239 of 424)	94.8 % (184 of 194)
Sidechain	20.7 % (168 of 812)	20.9 % (167 of 800)	8.3 % (1 of 12)
Aromatic	10.5 % (9 of 86)	10.5 % (9 of 86)
Methyl	28.3 % (34 of 120)	28.3 % (34 of 120)

1. Cu(II) Plastocyanin from Anabeana Variabilis

METYTVKLGS DKGLLVFEPA KLTIKPGDTV EFLNNKVPPH NVVFDAALNP AKSADLAKSL SHKQLLMSPG QSTSTTFPAD APAGEYTFYC EPHRGAGMVG KITVAG

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 7.80 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Cu(II) Plastocyanin from Anabeana Variabilis	[U-95% 15N]		1.0 mM
2	natrium ascorbate			0.1 mM

Chem. Shift Complete3

Sequence coverage: 91.5 %, Completeness: 41.4 %, Completeness (bb): 68.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	41.4 % (888 of 2145)	33.3 % (611 of 1836)	89.6 % (277 of 309)
Backbone	68.4 % (634 of 927)	56.4 % (359 of 636)	94.5 % (275 of 291)
Sidechain	20.9 % (254 of 1218)	21.0 % (252 of 1200)	11.1 % (2 of 18)
Aromatic	7.0 % (9 of 129)	7.0 % (9 of 129)
Methyl	26.1 % (47 of 180)	26.1 % (47 of 180)

1. Cu(II) Plastocyanin from Anabeana Variabilis

METYTVKLGS DKGLLVFEPA KLTIKPGDTV EFLNNKVPPH NVVFDAALNP AKSADLAKSL SHKQLLMSPG QSTSTTFPAD APAGEYTFYC EPHRGAGMVG KITVAG

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 7.46 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Cu(II) Plastocyanin from Anabeana Variabilis	[U-95% 15N]		1.0 mM
2	natrium ascorbate			0.1 mM

Chem. Shift Complete4

Sequence coverage: 90.6 %, Completeness: 40.7 %, Completeness (bb): 68.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	40.7 % (1163 of 2860)	32.3 % (791 of 2448)	90.3 % (372 of 412)
Backbone	68.1 % (842 of 1236)	55.8 % (473 of 848)	95.1 % (369 of 388)
Sidechain	19.8 % (321 of 1624)	19.9 % (318 of 1600)	12.5 % (3 of 24)
Aromatic	7.6 % (13 of 172)	7.6 % (13 of 172)
Methyl	24.6 % (59 of 240)	24.6 % (59 of 240)

1. Cu(II) Plastocyanin from Anabeana Variabilis

METYTVKLGS DKGLLVFEPA KLTIKPGDTV EFLNNKVPPH NVVFDAALNP AKSADLAKSL SHKQLLMSPG QSTSTTFPAD APAGEYTFYC EPHRGAGMVG KITVAG

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.96 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Cu(II) Plastocyanin from Anabeana Variabilis	[U-95% 15N]		1.0 mM
2	natrium ascorbate			0.1 mM

Chem. Shift Complete5

Sequence coverage: 92.5 %, Completeness: 40.6 %, Completeness (bb): 68.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	40.6 % (1453 of 3575)	32.2 % (985 of 3060)	90.9 % (468 of 515)
Backbone	68.4 % (1057 of 1545)	55.9 % (593 of 1060)	95.7 % (464 of 485)
Sidechain	19.5 % (396 of 2030)	19.6 % (392 of 2000)	13.3 % (4 of 30)
Aromatic	8.4 % (18 of 215)	8.4 % (18 of 215)
Methyl	22.7 % (68 of 300)	22.7 % (68 of 300)

1. Cu(II) Plastocyanin from Anabeana Variabilis

METYTVKLGS DKGLLVFEPA KLTIKPGDTV EFLNNKVPPH NVVFDAALNP AKSADLAKSL SHKQLLMSPG QSTSTTFPAD APAGEYTFYC EPHRGAGMVG KITVAG

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.59 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Cu(II) Plastocyanin from Anabeana Variabilis	[U-95% 15N]		1.0 mM
2	natrium ascorbate			0.1 mM

Chem. Shift Complete6

Sequence coverage: 92.5 %, Completeness: 41.2 %, Completeness (bb): 69.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	41.2 % (1768 of 4290)	32.8 % (1203 of 3672)	91.4 % (565 of 618)
Backbone	69.0 % (1279 of 1854)	56.5 % (719 of 1272)	96.2 % (560 of 582)
Sidechain	20.1 % (489 of 2436)	20.2 % (484 of 2400)	13.9 % (5 of 36)
Aromatic	8.9 % (23 of 258)	8.9 % (23 of 258)
Methyl	23.9 % (86 of 360)	23.9 % (86 of 360)

1. Cu(II) Plastocyanin from Anabeana Variabilis

METYTVKLGS DKGLLVFEPA KLTIKPGDTV EFLNNKVPPH NVVFDAALNP AKSADLAKSL SHKQLLMSPG QSTSTTFPAD APAGEYTFYC EPHRGAGMVG KITVAG

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.05 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Cu(II) Plastocyanin from Anabeana Variabilis	[U-95% 15N]		1.0 mM
2	natrium ascorbate			0.1 mM

Chem. Shift Complete7

Sequence coverage: 90.6 %, Completeness: 40.9 %, Completeness (bb): 69.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	40.9 % (2045 of 5005)	32.3 % (1383 of 4284)	91.8 % (662 of 721)
Backbone	69.0 % (1492 of 2163)	56.3 % (836 of 1484)	96.6 % (656 of 679)
Sidechain	19.5 % (553 of 2842)	19.5 % (547 of 2800)	14.3 % (6 of 42)
Aromatic	9.0 % (27 of 301)	9.0 % (27 of 301)
Methyl	22.9 % (96 of 420)	22.9 % (96 of 420)

1. Cu(II) Plastocyanin from Anabeana Variabilis

METYTVKLGS DKGLLVFEPA KLTIKPGDTV EFLNNKVPPH NVVFDAALNP AKSADLAKSL SHKQLLMSPG QSTSTTFPAD APAGEYTFYC EPHRGAGMVG KITVAG

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 5.78 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Cu(II) Plastocyanin from Anabeana Variabilis	[U-95% 15N]		1.0 mM
2	natrium ascorbate			0.1 mM

Chem. Shift Complete8

Sequence coverage: 88.7 %, Completeness: 40.5 %, Completeness (bb): 68.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	40.5 % (2319 of 5720)	31.9 % (1561 of 4896)	92.0 % (758 of 824)
Backbone	68.7 % (1699 of 2472)	56.0 % (949 of 1696)	96.6 % (750 of 776)
Sidechain	19.1 % (620 of 3248)	19.1 % (612 of 3200)	16.7 % (8 of 48)
Aromatic	7.8 % (27 of 344)	7.8 % (27 of 344)
Methyl	24.2 % (116 of 480)	24.2 % (116 of 480)

1. Cu(II) Plastocyanin from Anabeana Variabilis

METYTVKLGS DKGLLVFEPA KLTIKPGDTV EFLNNKVPPH NVVFDAALNP AKSADLAKSL SHKQLLMSPG QSTSTTFPAD APAGEYTFYC EPHRGAGMVG KITVAG

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 5.35 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Cu(II) Plastocyanin from Anabeana Variabilis	[U-95% 15N]		1.0 mM
2	natrium ascorbate			0.1 mM

Chem. Shift Complete9

Sequence coverage: 91.5 %, Completeness: 41.1 %, Completeness (bb): 69.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	41.1 % (2645 of 6435)	32.5 % (1791 of 5508)	92.1 % (854 of 927)
Backbone	69.2 % (1924 of 2781)	56.6 % (1079 of 1908)	96.8 % (845 of 873)
Sidechain	19.8 % (722 of 3654)	19.8 % (713 of 3600)	16.7 % (9 of 54)
Aromatic	7.0 % (27 of 387)	7.0 % (27 of 387)
Methyl	24.6 % (133 of 540)	24.6 % (133 of 540)

1. Cu(II) Plastocyanin from Anabeana Variabilis

METYTVKLGS DKGLLVFEPA KLTIKPGDTV EFLNNKVPPH NVVFDAALNP AKSADLAKSL SHKQLLMSPG QSTSTTFPAD APAGEYTFYC EPHRGAGMVG KITVAG

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 4.98 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Cu(II) Plastocyanin from Anabeana Variabilis	[U-95% 15N]		1.0 mM
2	natrium ascorbate			0.1 mM

Chem. Shift Complete10

Sequence coverage: 90.6 %, Completeness: 41.3 %, Completeness (bb): 69.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	41.3 % (2956 of 7150)	32.8 % (2005 of 6120)	92.3 % (951 of 1030)
Backbone	69.3 % (2141 of 3090)	56.7 % (1201 of 2120)	96.9 % (940 of 970)
Sidechain	20.1 % (817 of 4060)	20.1 % (806 of 4000)	18.3 % (11 of 60)
Aromatic	9.8 % (42 of 430)	9.8 % (42 of 430)
Methyl	24.7 % (148 of 600)	24.7 % (148 of 600)

1. Cu(II) Plastocyanin from Anabeana Variabilis

METYTVKLGS DKGLLVFEPA KLTIKPGDTV EFLNNKVPPH NVVFDAALNP AKSADLAKSL SHKQLLMSPG QSTSTTFPAD APAGEYTFYC EPHRGAGMVG KITVAG

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 4.60 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Cu(II) Plastocyanin from Anabeana Variabilis	[U-95% 15N]		1.0 mM
2	natrium ascorbate			0.1 mM

Chem. Shift Complete11

Sequence coverage: 90.6 %, Completeness: 41.3 %, Completeness (bb): 69.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	41.3 % (3249 of 7865)	32.7 % (2200 of 6732)	92.6 % (1049 of 1133)
Backbone	69.3 % (2357 of 3399)	56.7 % (1322 of 2332)	97.0 % (1035 of 1067)
Sidechain	20.0 % (894 of 4466)	20.0 % (880 of 4400)	21.2 % (14 of 66)
Aromatic	10.1 % (48 of 473)	10.1 % (48 of 473)
Methyl	23.9 % (158 of 660)	23.9 % (158 of 660)

1. Cu(II) Plastocyanin from Anabeana Variabilis

METYTVKLGS DKGLLVFEPA KLTIKPGDTV EFLNNKVPPH NVVFDAALNP AKSADLAKSL SHKQLLMSPG QSTSTTFPAD APAGEYTFYC EPHRGAGMVG KITVAG

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 4.20 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Cu(II) Plastocyanin from Anabeana Variabilis	[U-95% 15N]		1.0 mM
2	natrium ascorbate			0.1 mM

Chem. Shift Complete12

Sequence coverage: 91.5 %, Completeness: 41.4 %, Completeness (bb): 69.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	41.4 % (3552 of 8580)	32.8 % (2407 of 7344)	92.6 % (1145 of 1236)
Backbone	69.6 % (2580 of 3708)	57.0 % (1449 of 2544)	97.2 % (1131 of 1164)
Sidechain	20.0 % (974 of 4872)	20.0 % (960 of 4800)	19.4 % (14 of 72)
Aromatic	9.7 % (50 of 516)	9.7 % (50 of 516)
Methyl	24.3 % (175 of 720)	24.3 % (175 of 720)

1. Cu(II) Plastocyanin from Anabeana Variabilis

METYTVKLGS DKGLLVFEPA KLTIKPGDTV EFLNNKVPPH NVVFDAALNP AKSADLAKSL SHKQLLMSPG QSTSTTFPAD APAGEYTFYC EPHRGAGMVG KITVAG

Show sample condition

Sample

Temperature 293 (±0.1) K, pH 5.78 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Cu(II) Plastocyanin from Anabeana Variabilis	[U-95% 15N]		1.0 mM
2	natrium ascorbate			0.1 mM

Chem. Shift Complete13

Sequence coverage: 89.6 %, Completeness: 40.9 %, Completeness (bb): 69.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	40.9 % (3803 of 9295)	32.2 % (2562 of 7956)	92.7 % (1241 of 1339)
Backbone	69.4 % (2789 of 4017)	56.7 % (1563 of 2756)	97.2 % (1226 of 1261)
Sidechain	19.2 % (1016 of 5278)	19.2 % (1001 of 5200)	19.2 % (15 of 78)
Aromatic	8.9 % (50 of 559)	8.9 % (50 of 559)
Methyl	23.8 % (186 of 780)	23.8 % (186 of 780)

1. Cu(II) Plastocyanin from Anabeana Variabilis

METYTVKLGS DKGLLVFEPA KLTIKPGDTV EFLNNKVPPH NVVFDAALNP AKSADLAKSL SHKQLLMSPG QSTSTTFPAD APAGEYTFYC EPHRGAGMVG KITVAG

Show sample condition

Sample

Temperature 303 (±0.1) K, pH 5.78 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Cu(II) Plastocyanin from Anabeana Variabilis	[U-95% 15N]		1.0 mM
2	natrium ascorbate			0.1 mM

Chem. Shift Complete14

Sequence coverage: 90.6 %, Completeness: 41.0 %, Completeness (bb): 69.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	41.0 % (4107 of 10010)	32.3 % (2771 of 8568)	92.6 % (1336 of 1442)
Backbone	69.5 % (3007 of 4326)	56.8 % (1686 of 2968)	97.3 % (1321 of 1358)
Sidechain	19.4 % (1102 of 5684)	19.4 % (1087 of 5600)	17.9 % (15 of 84)
Aromatic	10.0 % (60 of 602)	10.0 % (60 of 602)
Methyl	24.6 % (207 of 840)	24.6 % (207 of 840)

1. Cu(II) Plastocyanin from Anabeana Variabilis

METYTVKLGS DKGLLVFEPA KLTIKPGDTV EFLNNKVPPH NVVFDAALNP AKSADLAKSL SHKQLLMSPG QSTSTTFPAD APAGEYTFYC EPHRGAGMVG KITVAG

Show sample condition

Sample

Temperature 308 (±0.1) K, pH 5.78 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Cu(II) Plastocyanin from Anabeana Variabilis	[U-95% 15N]		1.0 mM
2	natrium ascorbate			0.1 mM

Heteronucl. T₁

742 T₁ values in 8 lists
Coherence Sz, Field strength (¹H) 800 MHz, Temperature 298 (±0.1) K, pH 6.96 (±0.05), 8.50 (±0.05), 6.59 (±0.05), 6.05 (±0.05), 7.80 (±0.05), 5.78 (±0.05), 7.46 (±0.05), 5.35 (±0.05) Detail

Heteronucl. T₂

570 T₂ values in 6 lists
Coherence S(+,-), Field strength (¹H) 800 MHz, Temperature 293 (±0.1) K, 308 (±0.1) K, 298 (±0.1) K, 303 (±0.1) K, pH 4.60 (±0.05), 4.20 (±0.05), 4.98 (±0.05), 5.78 (±0.05) Detail

Release date

2004-02-11

Citation 1

Characterization of micros-ms dynamics of proteins using a combined analysis of 15N NMR relaxation and chemical shift: conformational exchange in plastocyanin induced by histidine protonations
Hass, M.A.S., Thuesen, M.H., Christensen, H.E.M., Led, J.J.
J. Am. Chem. Soc. (2004), 126, 753-765, PubMed 14733549 , DOI 10.1021/ja030366m , Abstract

Show more 1 citaion

Citation 2

Backbone dynamics of reduced plastocyanin from the cyanobacterium Anabaena variabilis: regions involved in electron transfer have enhanced mobility
Ma, L., Hass, M.A., Vierick, N., Kristensen, S.M., Ulstrup, J., Led, J.J.
Biochemistry (2003), 42, 320-330, PubMed 12525159 , DOI 10.1021/bi020553h , Abstract

Hide non-primary 1 citaion

Related entities 1. Cu(II) Plastocyanin from Anabeana Variabilis, : 1 : 6 : 153 entities Detail

Interaction partners 1. Cu(II) Plastocyanin from Anabeana Variabilis, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 1 experiments Detail

Chemical shift validation 17 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts, Heteronucl T1 relaxation data, Heteronucl T2 relaxation data	bmr5858_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	True (see coodinates for details)
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:90:CYS:SG	2:1:CU1:CU	unknown	unknown	n/a

Non-standard residues

None

Content subtype: bmr5858_3.str

#	Content subtype	Saveframe	Status	# of rows	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	OK	176	83.0 (chain: 1, length: 106)
2	Assigned chemical shifts	shift_set_2	OK	180	84.9 (chain: 1, length: 106)
3	Assigned chemical shifts	shift_set_3	OK	182	85.8 (chain: 1, length: 106)
4	Assigned chemical shifts	shift_set_4	OK	188	88.7 (chain: 1, length: 106)
5	Assigned chemical shifts	shift_set_5	OK	190	89.6 (chain: 1, length: 106)
6	Assigned chemical shifts	shift_set_6	OK	190	89.6 (chain: 1, length: 106)
7	Assigned chemical shifts	shift_set_7	OK	190	89.6 (chain: 1, length: 106)
8	Assigned chemical shifts	shift_set_8	OK	188	88.7 (chain: 1, length: 106)
9	Assigned chemical shifts	shift_set_9	OK	190	89.6 (chain: 1, length: 106)
10	Assigned chemical shifts	shift_set_10	OK	190	89.6 (chain: 1, length: 106)
11	Assigned chemical shifts	shift_set_11	OK	190	89.6 (chain: 1, length: 106)
12	Assigned chemical shifts	shift_set_12	OK	190	89.6 (chain: 1, length: 106)
13	Assigned chemical shifts	shift_set_13	OK	190	89.6 (chain: 1, length: 106)
14	Assigned chemical shifts	shift_set_14	OK	190	89.6 (chain: 1, length: 106)

Assigned chemical shifts

Saveframe: shift_set_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 106, Sequence coverage: 83.0%
- Total number of assignments
  - ¹⁵N chemical shifts: 88
  - ¹H chemical shifts: 88
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: shift_set_2
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 106, Sequence coverage: 84.9%
- Total number of assignments
  - ¹⁵N chemical shifts: 90
  - ¹H chemical shifts: 90
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: shift_set_3
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 106, Sequence coverage: 85.8%
- Total number of assignments
  - ¹⁵N chemical shifts: 91
  - ¹H chemical shifts: 91
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: shift_set_4
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 106, Sequence coverage: 88.7%
- Total number of assignments
  - ¹⁵N chemical shifts: 94
  - ¹H chemical shifts: 94
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: shift_set_5
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 106, Sequence coverage: 89.6%
- Total number of assignments
  - ¹⁵N chemical shifts: 95
  - ¹H chemical shifts: 95
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: shift_set_6
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 106, Sequence coverage: 89.6%
- Total number of assignments
  - ¹⁵N chemical shifts: 95
  - ¹H chemical shifts: 95
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: shift_set_7
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 106, Sequence coverage: 89.6%
- Total number of assignments
  - ¹⁵N chemical shifts: 95
  - ¹H chemical shifts: 95
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: shift_set_8
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 106, Sequence coverage: 88.7%
- Total number of assignments
  - ¹⁵N chemical shifts: 94
  - ¹H chemical shifts: 94
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: shift_set_9
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 106, Sequence coverage: 89.6%
- Total number of assignments
  - ¹⁵N chemical shifts: 95
  - ¹H chemical shifts: 95
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: shift_set_10
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 106, Sequence coverage: 89.6%
- Total number of assignments
  - ¹⁵N chemical shifts: 95
  - ¹H chemical shifts: 95
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: shift_set_11
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 106, Sequence coverage: 89.6%
- Total number of assignments
  - ¹⁵N chemical shifts: 95
  - ¹H chemical shifts: 95
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: shift_set_12
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 106, Sequence coverage: 89.6%
- Total number of assignments
  - ¹⁵N chemical shifts: 95
  - ¹H chemical shifts: 95
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: shift_set_13
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 106, Sequence coverage: 89.6%
- Total number of assignments
  - ¹⁵N chemical shifts: 95
  - ¹H chemical shifts: 95
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: shift_set_14
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 106, Sequence coverage: 89.6%
- Total number of assignments
  - ¹⁵N chemical shifts: 95
  - ¹H chemical shifts: 95
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

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BMRB: 5858

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Related entities 1. Cu(II) Plastocyanin from Anabeana Variabilis, : 1 : 6 : 153 entities Detail

Interaction partners 1. Cu(II) Plastocyanin from Anabeana Variabilis, : 1 interactors Detail

Experiments performed 1 experiments Detail

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Chemical shift validation 17 contents Detail