BMRB: 5877

Solid State NMR Structure of the Major Coat Protein in Bacteriophage Pf1

Authors

Thiriot, D.S., Opella, S.J.

Assembly

Bacteriophage Pf1 major coat protein

Entity

1. Bacteriophage Pf1 major coat protein (polymer, Thiol state: not present), 46 monomers, 4609.367 Da Detail

GVIDTSAVES AITDGQGDMK AIGGYIVGAL VILAVAGLIY SMLRKA

Formula weight

4609.367 Da

Source organism

Pseudomonas virus Pf1

Exptl. method

NMR

Refine. method

CONSTRUCTION OF BACTERIOPHAGE MODEL BASED ON THE SOLID STATE NMR STRUCTURE OF THE MAJOR COAT PROTEIN MONOMER

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 93.5 %, Completeness: 91.5 %, Completeness (bb): 93.5 % Detail

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Polymer type: polypeptide(L)

	Total	15N
All	91.5 % (43 of 47)	91.5 % (43 of 47)
Backbone	93.5 % (43 of 46)	93.5 % (43 of 46)
Sidechain	0.0 % (0 of 1)	0.0 % (0 of 1)

1. Pf1 phage major coat protein

GVIDTSAVES AITDGQGDMK AIGGYIVGAL VILAVAGLIY SMLRKA

Show sample condition

Sample

Pressure 1 atm, Temperature 303 (±1) K, pH 8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Pf1 phage major coat protein	[U-15N]		50 mg/mL
2	sodium borate buffer			5 mM
3	H2O			0.0 ~ 0.0 M

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Protein Blocks Logo

Calculated from 27 models in PDB: 1PJF, Strand ID: A Detail

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Coupling constant

43 J values in 1 lists
Pressure 1 atm, Temperature 303 (±1) K, pH 8 (±0.2) Detail

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Pressure 1 atm, Temperature 303 (±1) K, pH 8 (±0.2)

List #1 coupling_constants_1

Release date

2004-10-24

Citation

Structure of the coat protein in Pf1 bacteriophage determined by solid-state NMR spectroscopy
Thiriot, D.S., Nevzorov, A.A., Zagyanskiy, L., Wu, C.H., Opella, S.J.
J. Mol. Biol. (2004), 341, 869-879, PubMed 15288792 , DOI 10.1016/j.jmb.2004.06.038 , Abstract

Related entities 1. Bacteriophage Pf1 major coat protein, : 1 : 17 entities Detail

Experiments performed 2 experiments Detail