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BMRB: 5878

1SSF

1H, 13C and 15N NMR assignment of the region 1463-1617 of mouse p53 Binding Protein 1 (53BP1)

Authors

Charier, G., Alpha-Bazin, B., Couprie, J., Callebaut, I., Berenguer, F., Quemeneur, E., Gilquin, B., Zinn-Justin, S.

Assembly

region 1463-1617 of mouse 53BP1

Entity

1. region 1463-1617 of mouse 53BP1 (polymer, Thiol state: all free), 155 monomers, 17345.31 Da Detail

DSSSSGNSFV GLRVVAKWSS NGYFYSGKIT RDVGAGKYKL LFDDGYECDV LGKDILLCDP IPLDTEVTAL SEDEYFSAGV VKGHRKESGE LYYSIEKEGQ RKWYKRMAVI LSLEQGNRLR EQYGLGPYEA VTPLTKAADI SLDNLVEGKR KRRSN

Formula weight

17345.31 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 98.1 %, Completeness: 91.3 %, Completeness (bb): 94.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.3 % (1639 of 1795)	90.0 % (842 of 936)	93.0 % (649 of 698)	91.9 % (148 of 161)
Backbone	94.4 % (870 of 922)	93.5 % (301 of 322)	95.5 % (429 of 449)	92.7 % (140 of 151)
Sidechain	88.5 % (896 of 1012)	87.1 % (535 of 614)	91.0 % (353 of 388)	80.0 % (8 of 10)
Aromatic	99.3 % (147 of 148)	100.0 % (74 of 74)	98.6 % (71 of 72)	100.0 % (2 of 2)
Methyl	92.4 % (146 of 158)	91.1 % (72 of 79)	93.7 % (74 of 79)

1. p53 Binding Protein 1

DSSSSGNSFV GLRVVAKWSS NGYFYSGKIT RDVGAGKYKL LFDDGYECDV LGKDILLCDP IPLDTEVTAL SEDEYFSAGV VKGHRKESGE LYYSIEKEGQ RKWYKRMAVI LSLEQGNRLR EQYGLGPYEA VTPLTKAADI SLDNLVEGKR KRRSN

Show sample condition

Sample

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
2	Tris-HCl		50 mM
3	NaCl		150 mM
4	D2O		100 %

Chem. Shift Complete2

Sequence coverage: 39.4 %, Completeness: 55.4 %, Completeness (bb): 61.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	55.4 % (1990 of 3590)	54.1 % (1012 of 1872)	55.1 % (769 of 1396)	64.9 % (209 of 322)
Backbone	61.7 % (1138 of 1844)	62.6 % (403 of 644)	59.5 % (534 of 898)	66.6 % (201 of 302)
Sidechain	49.9 % (1009 of 2024)	49.1 % (603 of 1228)	51.2 % (397 of 776)	45.0 % (9 of 20)
Aromatic	51.0 % (151 of 296)	52.7 % (78 of 148)	49.3 % (71 of 144)	50.0 % (2 of 4)
Methyl	51.9 % (164 of 316)	51.3 % (81 of 158)	52.5 % (83 of 158)

1. p53 Binding Protein 1

DSSSSGNSFV GLRVVAKWSS NGYFYSGKIT RDVGAGKYKL LFDDGYECDV LGKDILLCDP IPLDTEVTAL SEDEYFSAGV VKGHRKESGE LYYSIEKEGQ RKWYKRMAVI LSLEQGNRLR EQYGLGPYEA VTPLTKAADI SLDNLVEGKR KRRSN

Show sample condition

Sample

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
2	Tris-HCl		50 mM
3	NaCl		150 mM
4	D2O		100 %

Protein Blocks Logo

Calculated from 10 models in PDB: 1SSF, Strand ID: A Detail

Release date

2004-02-10

Citation

1H, 13C and 15N resonance assignments of the region 1463-1617 of the mouse p53 binding protein 1 (53BP1)
Charier, G., Alpha-Bazin, B., Couprie, J., Callebaut, I., Berenguer, F., Quemeneur, E., Gilquin, B., Zinn-Justin, S.
J. Biomol. NMR (2004), 28, 303-304, PubMed 14752266 , DOI 10.1023/B:JNMR.0000013688.79211.8c , Abstract

Related entities 1. region 1463-1617 of mouse 53BP1, : 1 : 3 : 13 entities Detail

Interaction partners 1. region 1463-1617 of mouse 53BP1, : 19 interactors Detail

More details for 19 interactors identified by 10 citations

Experiments performed 14 experiments Detail

1 1H-15N HSQC

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
2	Tris-HCl		50 mM
3	NaCl		150 mM
4	D2O		100 %

Sample #2

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
5	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
6	Tris-HCl		50 mM
7	NaCl		150 mM
8	H2O		90 %
9	D2O		10 %

Sample #3

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
10	p53 Binding Protein 1	[U-95% 15N]	0.7 mM
11	Tris-HCl		50 mM
12	NaCl		150 mM
13	H2O		90 %
14	D2O		10 %

2 HNCO

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
2	Tris-HCl		50 mM
3	NaCl		150 mM
4	D2O		100 %

Sample #2

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
5	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
6	Tris-HCl		50 mM
7	NaCl		150 mM
8	H2O		90 %
9	D2O		10 %

Sample #3

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
10	p53 Binding Protein 1	[U-95% 15N]	0.7 mM
11	Tris-HCl		50 mM
12	NaCl		150 mM
13	H2O		90 %
14	D2O		10 %

3 HNCA

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
2	Tris-HCl		50 mM
3	NaCl		150 mM
4	D2O		100 %

Sample #2

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
5	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
6	Tris-HCl		50 mM
7	NaCl		150 mM
8	H2O		90 %
9	D2O		10 %

Sample #3

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
10	p53 Binding Protein 1	[U-95% 15N]	0.7 mM
11	Tris-HCl		50 mM
12	NaCl		150 mM
13	H2O		90 %
14	D2O		10 %

4 HN(CO)CA

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
2	Tris-HCl		50 mM
3	NaCl		150 mM
4	D2O		100 %

Sample #2

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
5	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
6	Tris-HCl		50 mM
7	NaCl		150 mM
8	H2O		90 %
9	D2O		10 %

Sample #3

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
10	p53 Binding Protein 1	[U-95% 15N]	0.7 mM
11	Tris-HCl		50 mM
12	NaCl		150 mM
13	H2O		90 %
14	D2O		10 %

5 CBCA(CO)NH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
2	Tris-HCl		50 mM
3	NaCl		150 mM
4	D2O		100 %

Sample #2

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
5	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
6	Tris-HCl		50 mM
7	NaCl		150 mM
8	H2O		90 %
9	D2O		10 %

Sample #3

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
10	p53 Binding Protein 1	[U-95% 15N]	0.7 mM
11	Tris-HCl		50 mM
12	NaCl		150 mM
13	H2O		90 %
14	D2O		10 %

6 CBCANH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
2	Tris-HCl		50 mM
3	NaCl		150 mM
4	D2O		100 %

Sample #2

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
5	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
6	Tris-HCl		50 mM
7	NaCl		150 mM
8	H2O		90 %
9	D2O		10 %

Sample #3

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
10	p53 Binding Protein 1	[U-95% 15N]	0.7 mM
11	Tris-HCl		50 mM
12	NaCl		150 mM
13	H2O		90 %
14	D2O		10 %

7 CBCACOHA

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
2	Tris-HCl		50 mM
3	NaCl		150 mM
4	D2O		100 %

Sample #2

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
5	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
6	Tris-HCl		50 mM
7	NaCl		150 mM
8	H2O		90 %
9	D2O		10 %

Sample #3

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
10	p53 Binding Protein 1	[U-95% 15N]	0.7 mM
11	Tris-HCl		50 mM
12	NaCl		150 mM
13	H2O		90 %
14	D2O		10 %

8 HNHA

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
2	Tris-HCl		50 mM
3	NaCl		150 mM
4	D2O		100 %

Sample #2

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
5	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
6	Tris-HCl		50 mM
7	NaCl		150 mM
8	H2O		90 %
9	D2O		10 %

Sample #3

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
10	p53 Binding Protein 1	[U-95% 15N]	0.7 mM
11	Tris-HCl		50 mM
12	NaCl		150 mM
13	H2O		90 %
14	D2O		10 %

9 (HCA)CO(CA)NH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
2	Tris-HCl		50 mM
3	NaCl		150 mM
4	D2O		100 %

Sample #2

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
5	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
6	Tris-HCl		50 mM
7	NaCl		150 mM
8	H2O		90 %
9	D2O		10 %

Sample #3

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
10	p53 Binding Protein 1	[U-95% 15N]	0.7 mM
11	Tris-HCl		50 mM
12	NaCl		150 mM
13	H2O		90 %
14	D2O		10 %

10 15N HSQC NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
2	Tris-HCl		50 mM
3	NaCl		150 mM
4	D2O		100 %

Sample #2

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
5	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
6	Tris-HCl		50 mM
7	NaCl		150 mM
8	H2O		90 %
9	D2O		10 %

Sample #3

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
10	p53 Binding Protein 1	[U-95% 15N]	0.7 mM
11	Tris-HCl		50 mM
12	NaCl		150 mM
13	H2O		90 %
14	D2O		10 %

11 HBHA(CO)NH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
2	Tris-HCl		50 mM
3	NaCl		150 mM
4	D2O		100 %

Sample #2

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
5	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
6	Tris-HCl		50 mM
7	NaCl		150 mM
8	H2O		90 %
9	D2O		10 %

Sample #3

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
10	p53 Binding Protein 1	[U-95% 15N]	0.7 mM
11	Tris-HCl		50 mM
12	NaCl		150 mM
13	H2O		90 %
14	D2O		10 %

12 HCCH TOSCY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
2	Tris-HCl		50 mM
3	NaCl		150 mM
4	D2O		100 %

Sample #2

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
5	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
6	Tris-HCl		50 mM
7	NaCl		150 mM
8	H2O		90 %
9	D2O		10 %

Sample #3

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
10	p53 Binding Protein 1	[U-95% 15N]	0.7 mM
11	Tris-HCl		50 mM
12	NaCl		150 mM
13	H2O		90 %
14	D2O		10 %

13 HCCH COSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
2	Tris-HCl		50 mM
3	NaCl		150 mM
4	D2O		100 %

Sample #2

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
5	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
6	Tris-HCl		50 mM
7	NaCl		150 mM
8	H2O		90 %
9	D2O		10 %

Sample #3

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
10	p53 Binding Protein 1	[U-95% 15N]	0.7 mM
11	Tris-HCl		50 mM
12	NaCl		150 mM
13	H2O		90 %
14	D2O		10 %

14 13C HSQC NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
2	Tris-HCl		50 mM
3	NaCl		150 mM
4	D2O		100 %

Sample #2

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
5	p53 Binding Protein 1	[U-95% 13C; U-95% 15N]	0.8 mM
6	Tris-HCl		50 mM
7	NaCl		150 mM
8	H2O		90 %
9	D2O		10 %

Sample #3

Temperature 300 (±0.2) K, pH 7.1 (±0.1)

#	Name	Isotope labeling	Concentration
10	p53 Binding Protein 1	[U-95% 15N]	0.7 mM
11	Tris-HCl		50 mM
12	NaCl		150 mM
13	H2O		90 %
14	D2O		10 %

nullKeywords DNA Damage, double strand break, signalling pathway, tudor domain

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Found 1 Document (3.855 seconds)

BMRB: 5878

Sample

Sample

Related entities 1. region 1463-1617 of mouse 53BP1, : 1 : 3 : 13 entities Detail

Interaction partners 1. region 1463-1617 of mouse 53BP1, : 19 interactors Detail

Experiments performed 14 experiments Detail

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