BMRBj - BMREntryMaster

Found 1 Document (1.3 seconds)

BMRB: 5886

6N2Y

Backbone 1H, 13C and 15N Chemical Shift Assignments for TF1 bata subunit

Authors

Yagi, H., Iwabuchi, T., Izumi, K., Yamazaki, T., Yoshida, M., Akutsu, H.

Assembly

TF1 ATPase Beta Subunit

Entity

1. TF1 ATPase Beta Subunit (polymer, Thiol state: not present), 473 monomers, 51852.53 Da Detail

MTRGRVIQVM GPVVDVKFEN GHLPAIYNAL KIQHKARNEN EVDIDLTLEV ALHLGDDTVR TIAMASTDGL IRGMEVIDTG APISVPVGEV TLGRVFNVLG EPIDLEGDIP ADARRDPIHR PAPKFEELAT EVEILETGIK VVDLLAPYIK GGKIGLFGGA GVGKTVLIQE LIHNIAQEHG GISVFAGVGE RTREGNDLYH EMKDSGVISK TAMVFGQMNE PPGARMRVAL TGLTMAEYFR DEQGQDVLLF IDNIFRFTQA GSEVSALLGR MPSAVGYQPT LATEMGQLQE RITSTAKGSI TSIQAIYVPA DDYTDPAPAT TFSHLDATTN LERKLAEMGI YPAVDPLAST SRALAPEIVG EEHYQVARKV QQTLQRYKEL QDIIAILGMD ELSDEDKLVV HRARRIQFFL SQNFHVAEQF TGQPGSYVPV KETVRGFKEI LEGKYDHLPE DAFRLVGRIE EVVEKAKAMG VEV

Formula weight

51852.53 Da

Source organism

Bacillus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 85.4 %, Completeness: 39.7 %, Completeness (bb): 63.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	39.7 % (2138 of 5386)	27.4 % (763 of 2784)	46.1 % (978 of 2120)	82.4 % (397 of 482)
Backbone	63.7 % (1779 of 2792)	51.7 % (499 of 966)	64.3 % (885 of 1376)	87.8 % (395 of 450)
Sidechain	14.8 % (447 of 3024)	14.7 % (267 of 1818)	15.2 % (178 of 1174)	6.3 % (2 of 32)
Aromatic	1.9 % (6 of 314)	3.8 % (6 of 157)	0.0 % (0 of 157)
Methyl	19.7 % (120 of 608)	20.7 % (63 of 304)	18.8 % (57 of 304)

1. TF1 ATPase Beta Subunit

Show sample condition

Sample #1

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 1-124a.a.

#	Name	Isotope labeling	Type	Concentration
1	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 1-124a.a.		0.3 mM

Sample #2

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 1-271a.a.

#	Name	Isotope labeling	Type	Concentration
2	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 1-271a.a.		0.3 mM

Sample #3

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 272-473a.a.

#	Name	Isotope labeling	Type	Concentration
3	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 272-473a.a.		0.3 mM

Sample #4

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 391-473a.a.

#	Name	Isotope labeling	Type	Concentration
4	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 391-473a.a.		0.3 mM

Release date

2005-05-02

Citation

Conformational change of H+-ATPase beta monomer revealed on segmental isotope labeling NMR spectroscopy
Yagi, H., Tsujimoto, T., Yamazaki, T., Yoshida, M., Akutsu, H.
J. Am. Chem. Soc. (2004), 126, 16632-16638, PubMed 15600369 , DOI 10.1021/ja045279o , Abstract

Related entities 1. TF1 ATPase Beta Subunit, : 1 : 20 : 3 : 4 : 238 entities Detail

Interaction partners 1. TF1 ATPase Beta Subunit, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 7 experiments Detail

1 1H-15N HSQC TROSY

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 1-124a.a.

#	Name	Isotope labeling	Type	Concentration
1	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 1-124a.a.		0.3 mM

Sample #2

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 1-271a.a.

#	Name	Isotope labeling	Type	Concentration
2	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 1-271a.a.		0.3 mM

Sample #3

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 272-473a.a.

#	Name	Isotope labeling	Type	Concentration
3	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 272-473a.a.		0.3 mM

Sample #4

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 391-473a.a.

#	Name	Isotope labeling	Type	Concentration
4	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 391-473a.a.		0.3 mM

2 HNCO TROSY

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 1-124a.a.

#	Name	Isotope labeling	Type	Concentration
1	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 1-124a.a.		0.3 mM

Sample #2

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 1-271a.a.

#	Name	Isotope labeling	Type	Concentration
2	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 1-271a.a.		0.3 mM

Sample #3

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 272-473a.a.

#	Name	Isotope labeling	Type	Concentration
3	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 272-473a.a.		0.3 mM

Sample #4

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 391-473a.a.

#	Name	Isotope labeling	Type	Concentration
4	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 391-473a.a.		0.3 mM

3 HNCA TROSY

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 1-124a.a.

#	Name	Isotope labeling	Type	Concentration
1	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 1-124a.a.		0.3 mM

Sample #2

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 1-271a.a.

#	Name	Isotope labeling	Type	Concentration
2	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 1-271a.a.		0.3 mM

Sample #3

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 272-473a.a.

#	Name	Isotope labeling	Type	Concentration
3	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 272-473a.a.		0.3 mM

Sample #4

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 391-473a.a.

#	Name	Isotope labeling	Type	Concentration
4	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 391-473a.a.		0.3 mM

4 HN(CO)CA TROSY

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 1-124a.a.

#	Name	Isotope labeling	Type	Concentration
1	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 1-124a.a.		0.3 mM

Sample #2

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 1-271a.a.

#	Name	Isotope labeling	Type	Concentration
2	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 1-271a.a.		0.3 mM

Sample #3

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 272-473a.a.

#	Name	Isotope labeling	Type	Concentration
3	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 272-473a.a.		0.3 mM

Sample #4

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 391-473a.a.

#	Name	Isotope labeling	Type	Concentration
4	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 391-473a.a.		0.3 mM

5 HNCACB TROSY

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 1-124a.a.

#	Name	Isotope labeling	Type	Concentration
1	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 1-124a.a.		0.3 mM

Sample #2

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 1-271a.a.

#	Name	Isotope labeling	Type	Concentration
2	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 1-271a.a.		0.3 mM

Sample #3

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 272-473a.a.

#	Name	Isotope labeling	Type	Concentration
3	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 272-473a.a.		0.3 mM

Sample #4

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 391-473a.a.

#	Name	Isotope labeling	Type	Concentration
4	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 391-473a.a.		0.3 mM

6 HN(CO)CACB TROSY

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 1-124a.a.

#	Name	Isotope labeling	Type	Concentration
1	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 1-124a.a.		0.3 mM

Sample #2

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 1-271a.a.

#	Name	Isotope labeling	Type	Concentration
2	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 1-271a.a.		0.3 mM

Sample #3

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 272-473a.a.

#	Name	Isotope labeling	Type	Concentration
3	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 272-473a.a.		0.3 mM

Sample #4

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 391-473a.a.

#	Name	Isotope labeling	Type	Concentration
4	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 391-473a.a.		0.3 mM

7 HN(CA)CO TROSY

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 1-124a.a.

#	Name	Isotope labeling	Type	Concentration
1	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 1-124a.a.		0.3 mM

Sample #2

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 1-271a.a.

#	Name	Isotope labeling	Type	Concentration
2	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 1-271a.a.		0.3 mM

Sample #3

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 272-473a.a.

#	Name	Isotope labeling	Type	Concentration
3	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 272-473a.a.		0.3 mM

Sample #4

Temperature 313 (±1) K, pH 8.0 (±0.3), Details Segmental labeling with with 13C, 15N, 2H labeling levels known: [95% 13C; 98% 15N; 80% 2H] 391-473a.a.

#	Name	Isotope labeling	Type	Concentration
4	TF1 ATPase Beta Subunit	[U-95% 13C; U-98% 15N; U-80% 2H] 391-473a.a.		0.3 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5886_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5886_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	Shift_set_1	Warning	1554		82.2 (chain: 1, length: 473)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: Shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 473, Sequence coverage: 82.2%
- Total number of assignments
  - ¹H chemical shifts: 389
  - ¹³C chemical shifts: 776
  - ¹⁵N chemical shifts: 389
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	318	0	0.0
¹³C chemical shifts	318	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	2784	389	14.0
¹³C chemical shifts	2120	776	36.6
¹⁵N chemical shifts	509	389	76.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	966	389	40.3
¹³C chemical shifts	946	776	82.0
¹⁵N chemical shifts	450	389	86.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1818	0	0.0
¹³C chemical shifts	1174	0	0.0
¹⁵N chemical shifts	59	0	0.0

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Found 1 Document (1.3 seconds)

BMRB: 5886

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Related entities 1. TF1 ATPase Beta Subunit, : 1 : 20 : 3 : 4 : 238 entities Detail

Interaction partners 1. TF1 ATPase Beta Subunit, : 2 interactors Detail

Experiments performed 7 experiments Detail

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Chemical shift validation 3 contents Detail