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Found 1 Document (1.359 seconds)

BMRB: 5887

1OUN

1H, 15N and 13C assignments for nuclear transport factor 2 (NTF2)

Authors

Morrison, J., Yang, J., Stewart, M., Neuhaus, D.

Assembly

nuclear transport factor 2

Entity

1. nuclear transport factor 2 (polymer, Thiol state: all free), 127 monomers, 14478.32 × 2 Da Detail

MGDKPIWEQI GSSFIQHYYQ LFDNDRTQLG AIYIDASCLT WEGQQFQGKA AIVEKLSSLP FQKIQHSITA QDHQPTPDSC IISMVVGQLK ADEDPIMGFH QMFLLKNIND AWVCTNDMFR LALHNFG

Total weight

28956.64 Da

Max. entity weight

14478.32 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 77.1 %, Completeness (bb): 84.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	77.1 % (1171 of 1519)	79.4 % (626 of 788)	69.2 % (407 of 588)	96.5 % (138 of 143)
Backbone	84.0 % (632 of 752)	96.9 % (249 of 257)	70.2 % (262 of 373)	99.2 % (121 of 122)
Sidechain	73.9 % (655 of 886)	71.0 % (377 of 531)	78.1 % (261 of 334)	81.0 % (17 of 21)
Aromatic	68.8 % (110 of 160)	71.2 % (57 of 80)	64.9 % (50 of 77)	100.0 % (3 of 3)
Methyl	90.8 % (118 of 130)	92.3 % (60 of 65)	89.2 % (58 of 65)

1. Nuclear transport factor 2

MGDKPIWEQI GSSFIQHYYQ LFDNDRTQLG AIYIDASCLT WEGQQFQGKA AIVEKLSSLP FQKIQHSITA QDHQPTPDSC IISMVVGQLK ADEDPIMGFH QMFLLKNIND AWVCTNDMFR LALHNFG

Show sample condition

Sample #1

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Concentration
1	Nuclear transport factor 2	0.4 ~ 1.0 mM
2	sodium phosphate	20 mM
3	sodium chloride	50 mM

Sample #2

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Nuclear transport factor 2	[U-98% 15N]	0.4 ~ 1.0 mM
5	sodium phosphate		20 mM
6	sodium chloride		50 mM

Sample #3

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Nuclear transport factor 2	[U-98% 13C; U-98% 15N]	0.4 ~ 1.0 mM
8	sodium phosphate		20 mM
9	sodium chloride		50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 2.43 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 2.43 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.07 ppm, Outliers: 2 Detail

Release date

2005-09-15

Citation

Solution NMR study of the interaction between NTF2 and nucleoporin FxFG repeats
Morrison, J., Yang, J., Stewart, M., Neuhaus, D.
J. Mol. Biol. (2003), 333, 587-603, PubMed 14556747 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 5888 released on 2003-12-18
    Title Backbone 1H, 15N, CA and CB assignments for the W7A mutant of rat nuclear transport factor 2 (NTF2)
  BMRB: 5889 released on 2003-12-18
    Title 1H assignments for yeast nucleoporin Nsp1 residues 528-557

Related entities 1. nuclear transport factor 2, : 1 : 6 : 3 : 6 : 130 entities Detail

Interaction partners 1. nuclear transport factor 2, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 13 experiments Detail

1 2D (1H, 1H) NOESY

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Concentration
1	Nuclear transport factor 2	0.4 ~ 1.0 mM
2	sodium phosphate	20 mM
3	sodium chloride	50 mM

Sample #2

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Nuclear transport factor 2	[U-98% 15N]	0.4 ~ 1.0 mM
5	sodium phosphate		20 mM
6	sodium chloride		50 mM

Sample #3

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Nuclear transport factor 2	[U-98% 13C; U-98% 15N]	0.4 ~ 1.0 mM
8	sodium phosphate		20 mM
9	sodium chloride		50 mM

2 2D (1H, 1H) TOCSY

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Concentration
1	Nuclear transport factor 2	0.4 ~ 1.0 mM
2	sodium phosphate	20 mM
3	sodium chloride	50 mM

Sample #2

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Nuclear transport factor 2	[U-98% 15N]	0.4 ~ 1.0 mM
5	sodium phosphate		20 mM
6	sodium chloride		50 mM

Sample #3

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Nuclear transport factor 2	[U-98% 13C; U-98% 15N]	0.4 ~ 1.0 mM
8	sodium phosphate		20 mM
9	sodium chloride		50 mM

3 2D (1H, 1H) 2Q correlation

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Concentration
1	Nuclear transport factor 2	0.4 ~ 1.0 mM
2	sodium phosphate	20 mM
3	sodium chloride	50 mM

Sample #2

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Nuclear transport factor 2	[U-98% 15N]	0.4 ~ 1.0 mM
5	sodium phosphate		20 mM
6	sodium chloride		50 mM

Sample #3

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Nuclear transport factor 2	[U-98% 13C; U-98% 15N]	0.4 ~ 1.0 mM
8	sodium phosphate		20 mM
9	sodium chloride		50 mM

4 2D 15N-HSQC

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Concentration
1	Nuclear transport factor 2	0.4 ~ 1.0 mM
2	sodium phosphate	20 mM
3	sodium chloride	50 mM

Sample #2

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Nuclear transport factor 2	[U-98% 15N]	0.4 ~ 1.0 mM
5	sodium phosphate		20 mM
6	sodium chloride		50 mM

Sample #3

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Nuclear transport factor 2	[U-98% 13C; U-98% 15N]	0.4 ~ 1.0 mM
8	sodium phosphate		20 mM
9	sodium chloride		50 mM

5 2D 13C-HSQC

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Concentration
1	Nuclear transport factor 2	0.4 ~ 1.0 mM
2	sodium phosphate	20 mM
3	sodium chloride	50 mM

Sample #2

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Nuclear transport factor 2	[U-98% 15N]	0.4 ~ 1.0 mM
5	sodium phosphate		20 mM
6	sodium chloride		50 mM

Sample #3

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Nuclear transport factor 2	[U-98% 13C; U-98% 15N]	0.4 ~ 1.0 mM
8	sodium phosphate		20 mM
9	sodium chloride		50 mM

6 3D HNCACB

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Concentration
1	Nuclear transport factor 2	0.4 ~ 1.0 mM
2	sodium phosphate	20 mM
3	sodium chloride	50 mM

Sample #2

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Nuclear transport factor 2	[U-98% 15N]	0.4 ~ 1.0 mM
5	sodium phosphate		20 mM
6	sodium chloride		50 mM

Sample #3

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Nuclear transport factor 2	[U-98% 13C; U-98% 15N]	0.4 ~ 1.0 mM
8	sodium phosphate		20 mM
9	sodium chloride		50 mM

7 3D CBCA(CO)NH

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Concentration
1	Nuclear transport factor 2	0.4 ~ 1.0 mM
2	sodium phosphate	20 mM
3	sodium chloride	50 mM

Sample #2

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Nuclear transport factor 2	[U-98% 15N]	0.4 ~ 1.0 mM
5	sodium phosphate		20 mM
6	sodium chloride		50 mM

Sample #3

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Nuclear transport factor 2	[U-98% 13C; U-98% 15N]	0.4 ~ 1.0 mM
8	sodium phosphate		20 mM
9	sodium chloride		50 mM

8 3D HNHAHB

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Concentration
1	Nuclear transport factor 2	0.4 ~ 1.0 mM
2	sodium phosphate	20 mM
3	sodium chloride	50 mM

Sample #2

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Nuclear transport factor 2	[U-98% 15N]	0.4 ~ 1.0 mM
5	sodium phosphate		20 mM
6	sodium chloride		50 mM

Sample #3

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Nuclear transport factor 2	[U-98% 13C; U-98% 15N]	0.4 ~ 1.0 mM
8	sodium phosphate		20 mM
9	sodium chloride		50 mM

9 3D HBHA(CO)NH

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Concentration
1	Nuclear transport factor 2	0.4 ~ 1.0 mM
2	sodium phosphate	20 mM
3	sodium chloride	50 mM

Sample #2

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Nuclear transport factor 2	[U-98% 15N]	0.4 ~ 1.0 mM
5	sodium phosphate		20 mM
6	sodium chloride		50 mM

Sample #3

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Nuclear transport factor 2	[U-98% 13C; U-98% 15N]	0.4 ~ 1.0 mM
8	sodium phosphate		20 mM
9	sodium chloride		50 mM

10 3D 15N NOESY-HSQC

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Concentration
1	Nuclear transport factor 2	0.4 ~ 1.0 mM
2	sodium phosphate	20 mM
3	sodium chloride	50 mM

Sample #2

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Nuclear transport factor 2	[U-98% 15N]	0.4 ~ 1.0 mM
5	sodium phosphate		20 mM
6	sodium chloride		50 mM

Sample #3

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Nuclear transport factor 2	[U-98% 13C; U-98% 15N]	0.4 ~ 1.0 mM
8	sodium phosphate		20 mM
9	sodium chloride		50 mM

11 3D 13C NOESY-HSQC

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Concentration
1	Nuclear transport factor 2	0.4 ~ 1.0 mM
2	sodium phosphate	20 mM
3	sodium chloride	50 mM

Sample #2

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Nuclear transport factor 2	[U-98% 15N]	0.4 ~ 1.0 mM
5	sodium phosphate		20 mM
6	sodium chloride		50 mM

Sample #3

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Nuclear transport factor 2	[U-98% 13C; U-98% 15N]	0.4 ~ 1.0 mM
8	sodium phosphate		20 mM
9	sodium chloride		50 mM

12 3D HCCH-TOCSY

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Concentration
1	Nuclear transport factor 2	0.4 ~ 1.0 mM
2	sodium phosphate	20 mM
3	sodium chloride	50 mM

Sample #2

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Nuclear transport factor 2	[U-98% 15N]	0.4 ~ 1.0 mM
5	sodium phosphate		20 mM
6	sodium chloride		50 mM

Sample #3

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Nuclear transport factor 2	[U-98% 13C; U-98% 15N]	0.4 ~ 1.0 mM
8	sodium phosphate		20 mM
9	sodium chloride		50 mM

13 3D H(CCCO)NH-TOCSY

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Concentration
1	Nuclear transport factor 2	0.4 ~ 1.0 mM
2	sodium phosphate	20 mM
3	sodium chloride	50 mM

Sample #2

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Nuclear transport factor 2	[U-98% 15N]	0.4 ~ 1.0 mM
5	sodium phosphate		20 mM
6	sodium chloride		50 mM

Sample #3

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Nuclear transport factor 2	[U-98% 13C; U-98% 15N]	0.4 ~ 1.0 mM
8	sodium phosphate		20 mM
9	sodium chloride		50 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5887_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5887_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	1227		99.2 (chain: 1, length: 127)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 127, Sequence coverage: 99.2%
- Total number of assignments
  - ¹H chemical shifts: 710
  - ¹³C chemical shifts: 379
  - ¹⁵N chemical shifts: 138
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	788	586	74.4
¹³C chemical shifts	588	379	64.5
¹⁵N chemical shifts	145	138	95.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	257	246	95.7
¹³C chemical shifts	254	125	49.2
¹⁵N chemical shifts	122	121	99.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	531	340	64.0
¹³C chemical shifts	334	254	76.0
¹⁵N chemical shifts	23	17	73.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	80	55	68.8
¹³C chemical shifts	77	48	62.3
¹⁵N chemical shifts	3	3	100.0

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Found 1 Document (1.359 seconds)

BMRB: 5887

Sample #1

Sample #2

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. nuclear transport factor 2, : 1 : 6 : 3 : 6 : 130 entities Detail

Interaction partners 1. nuclear transport factor 2, : 1 interactors Detail

Experiments performed 13 experiments Detail

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