BMRBj - BMREntryMaster

Found 1 Document (1.308 seconds)

BMRB: 5888

1QMA

Backbone 1H, 15N, CA and CB assignments for the W7A mutant of rat nuclear transport factor 2 (NTF2)

Authors

Morrison, J., Yang, J., Stewart, M., Neuhaus, D.

Assembly

nuclear transport factor 2 W7A mutant

Entity

1. nuclear transport factor 2 W7A mutant (polymer, Thiol state: all free), 127 monomers, 14363.19 × 2 Da Detail

MGDKPIAEQI GSSFIQHYYQ LFDNDRTQLG AIYIDASCLT WEGQQFQGKA AIVEKLSSLP FQKIQHSITA QDHQPTPDSC IISMVVGQLK ADEDPIMGFH QMFLLKNIND AWVCTNDMFR LALHNFG

Total weight

28726.38 Da

Max. entity weight

14363.19 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 37.6 %, Completeness (bb): 66.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	37.6 % (566 of 1506)	23.0 % (180 of 781)	44.1 % (257 of 583)	90.8 % (129 of 142)
Backbone	66.0 % (496 of 752)	50.6 % (130 of 257)	66.0 % (246 of 373)	98.4 % (120 of 122)
Sidechain	20.7 % (181 of 873)	9.4 % (49 of 524)	37.7 % (124 of 329)	40.0 % (8 of 20)
Aromatic	5.4 % (8 of 148)	5.4 % (4 of 74)	5.6 % (4 of 72)	0.0 % (0 of 2)
Methyl	11.4 % (15 of 132)	4.5 % (3 of 66)	18.2 % (12 of 66)

1. Nuclear transport factor 2 W7A mutant

MGDKPIAEQI GSSFIQHYYQ LFDNDRTQLG AIYIDASCLT WEGQQFQGKA AIVEKLSSLP FQKIQHSITA QDHQPTPDSC IISMVVGQLK ADEDPIMGFH QMFLLKNIND AWVCTNDMFR LALHNFG

Show sample condition

Sample #1

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Concentration
1	Nuclear transport factor 2 W7A mutant	0.4 ~ 1.0 mM
2	sodium phosphate	20 mM
3	sodium chloride	50 mM

Sample #2

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Nuclear transport factor 2 W7A mutant	[U-98% 15N]	0.4 ~ 1.0 mM
5	sodium phosphate		20 mM
6	sodium chloride		50 mM

Sample #3

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Nuclear transport factor 2 W7A mutant	[U-98% 13C; U-98% 15N]	0.4 ~ 1.0 mM
8	sodium phosphate		20 mM
9	sodium chloride		50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 2.6 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 2.6 ppm, Outliers: 1 Detail

Release date

2003-12-18

Citation

Solution NMR study of the interaction between NTF2 and nucleoporin FxFG repeats
Morrison, J., Yang, J., Stewart, M., Neuhaus, D.
J. Mol. Biol. (2003), 333, 587-603, PubMed 14556747 , DOI 10.1016/j.jmb.2003.08.050 , Abstract

Related entities 1. nuclear transport factor 2 W7A mutant, : 1 : 12 : 135 entities Detail

Interaction partners 1. nuclear transport factor 2 W7A mutant, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 6 experiments Detail

1 2D 15N-HSQC

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Concentration
1	Nuclear transport factor 2 W7A mutant	0.4 ~ 1.0 mM
2	sodium phosphate	20 mM
3	sodium chloride	50 mM

Sample #2

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Nuclear transport factor 2 W7A mutant	[U-98% 15N]	0.4 ~ 1.0 mM
5	sodium phosphate		20 mM
6	sodium chloride		50 mM

Sample #3

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Nuclear transport factor 2 W7A mutant	[U-98% 13C; U-98% 15N]	0.4 ~ 1.0 mM
8	sodium phosphate		20 mM
9	sodium chloride		50 mM

2 2D 13C-HSQC

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Concentration
1	Nuclear transport factor 2 W7A mutant	0.4 ~ 1.0 mM
2	sodium phosphate	20 mM
3	sodium chloride	50 mM

Sample #2

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Nuclear transport factor 2 W7A mutant	[U-98% 15N]	0.4 ~ 1.0 mM
5	sodium phosphate		20 mM
6	sodium chloride		50 mM

Sample #3

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Nuclear transport factor 2 W7A mutant	[U-98% 13C; U-98% 15N]	0.4 ~ 1.0 mM
8	sodium phosphate		20 mM
9	sodium chloride		50 mM

3 3D HNCACB

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Concentration
1	Nuclear transport factor 2 W7A mutant	0.4 ~ 1.0 mM
2	sodium phosphate	20 mM
3	sodium chloride	50 mM

Sample #2

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Nuclear transport factor 2 W7A mutant	[U-98% 15N]	0.4 ~ 1.0 mM
5	sodium phosphate		20 mM
6	sodium chloride		50 mM

Sample #3

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Nuclear transport factor 2 W7A mutant	[U-98% 13C; U-98% 15N]	0.4 ~ 1.0 mM
8	sodium phosphate		20 mM
9	sodium chloride		50 mM

4 3D CBCA(CO)NH

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Concentration
1	Nuclear transport factor 2 W7A mutant	0.4 ~ 1.0 mM
2	sodium phosphate	20 mM
3	sodium chloride	50 mM

Sample #2

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Nuclear transport factor 2 W7A mutant	[U-98% 15N]	0.4 ~ 1.0 mM
5	sodium phosphate		20 mM
6	sodium chloride		50 mM

Sample #3

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Nuclear transport factor 2 W7A mutant	[U-98% 13C; U-98% 15N]	0.4 ~ 1.0 mM
8	sodium phosphate		20 mM
9	sodium chloride		50 mM

5 3D HNHAHB

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Concentration
1	Nuclear transport factor 2 W7A mutant	0.4 ~ 1.0 mM
2	sodium phosphate	20 mM
3	sodium chloride	50 mM

Sample #2

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Nuclear transport factor 2 W7A mutant	[U-98% 15N]	0.4 ~ 1.0 mM
5	sodium phosphate		20 mM
6	sodium chloride		50 mM

Sample #3

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Nuclear transport factor 2 W7A mutant	[U-98% 13C; U-98% 15N]	0.4 ~ 1.0 mM
8	sodium phosphate		20 mM
9	sodium chloride		50 mM

6 3D HBHA(CO)NH

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (500 MHz)

Sample #1

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Concentration
1	Nuclear transport factor 2 W7A mutant	0.4 ~ 1.0 mM
2	sodium phosphate	20 mM
3	sodium chloride	50 mM

Sample #2

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Nuclear transport factor 2 W7A mutant	[U-98% 15N]	0.4 ~ 1.0 mM
5	sodium phosphate		20 mM
6	sodium chloride		50 mM

Sample #3

Temperature 300 (±0.5) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	Nuclear transport factor 2 W7A mutant	[U-98% 13C; U-98% 15N]	0.4 ~ 1.0 mM
8	sodium phosphate		20 mM
9	sodium chloride		50 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5888_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5888_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	502		99.2 (chain: 1, length: 127)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 127, Sequence coverage: 99.2%
- Total number of assignments
  - ¹H chemical shifts: 136
  - ¹³C chemical shifts: 237
  - ¹⁵N chemical shifts: 129
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	257	121	47.1
¹³C chemical shifts	254	123	48.4
¹⁵N chemical shifts	122	121	99.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	71	0	0.0
¹³C chemical shifts	71	9	12.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	781	136	17.4
¹³C chemical shifts	583	237	40.7
¹⁵N chemical shifts	144	129	89.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	524	15	2.9
¹³C chemical shifts	329	114	34.7
¹⁵N chemical shifts	22	8	36.4

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.308 seconds)

BMRB: 5888

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Related entities 1. nuclear transport factor 2 W7A mutant, : 1 : 12 : 135 entities Detail

Interaction partners 1. nuclear transport factor 2 W7A mutant, : 1 interactors Detail

Experiments performed 6 experiments Detail

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Chemical shift validation 3 contents Detail