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Found 1 Document (1.645 seconds)

BMRB: 5897

1Q59

Solution Structure of the BHRF1 Protein From Epstein-Barr Virus, a Homolog of Human Bcl-2

Authors

Olejniczak, E.T.

Assembly

homolog Bcl-2 Epstein Barr Virus

Entity

1. homolog Bcl-2 Epstein Barr Virus (polymer, Thiol state: not reported), 172 monomers, 19822.02 Da Detail

MAYSTREILL ALCIRDSRVH GNGTLHPVLE LAARETPLRL SPEDTVVLRY HVLLEEIIER NSETFTETWN RFITHTEHVD LDFNSVFLEI FHRGDPSLGR ALAWMAWCMH ACRTLCCNQS TPYYVVDLSV RGMLEASEGL DGWIHQQGGW STLIEDNIPG DDDDLEHHHH HH

Formula weight

19822.02 Da

Source organism

Human herpesvirus 4 strain B95-8

Exptl. method

NMR

Refine. method

BHRF1 structures were calculated using a simulated annealing protocol with the program CNX

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.9 %, Completeness: 79.6 %, Completeness (bb): 87.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	79.6 % (1572 of 1976)	75.4 % (763 of 1012)	84.2 % (660 of 784)	82.8 % (149 of 180)
Backbone	87.1 % (888 of 1020)	87.1 % (303 of 348)	87.2 % (441 of 506)	86.7 % (144 of 166)
Sidechain	74.3 % (831 of 1118)	69.4 % (461 of 664)	83.0 % (365 of 440)	35.7 % (5 of 14)
Aromatic	67.7 % (134 of 198)	69.7 % (69 of 99)	66.0 % (62 of 94)	60.0 % (3 of 5)
Methyl	92.6 % (187 of 202)	93.1 % (94 of 101)	92.1 % (93 of 101)

1. bcl-2 homolog

MAYSTREILL ALCIRDSRVH GNGTLHPVLE LAARETPLRL SPEDTVVLRY HVLLEEIIER NSETFTETWN RFITHTEHVD LDFNSVFLEI FHRGDPSLGR ALAWMAWCMH ACRTLCCNQS TPYYVVDLSV RGMLEASEGL DGWIHQQGGW STLIEDNIPG DDDDLEHHHH HH

Show sample condition

Sample

Temperature 303 (±3) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	bcl-2 homolog			0.5 ~ 1.0 mM

Protein Blocks Logo

Calculated from 1 models in PDB: 1Q59, Strand ID: A Detail

Release date

2003-12-04

Citation

Solution structure of the BHRF1 protein from Epstein-Barr virus, a homolog of human Bcl-2
Huang, Q., Petros, A.M., Virgin, H.W., Fesik, S.W., Olejniczak, E.T.
J. Mol. Biol. (2003), 332, 1123-1130, PubMed 14499614 , DOI: , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P03182 released on 1986-07-21
Title EAR_EBVB9 Entity Apoptosis regulator BHRF1

Related entities 1. homolog Bcl-2 Epstein Barr Virus, : 1 : 8 : 1 entities Detail

Interaction partners 1. homolog Bcl-2 Epstein Barr Virus, : 5 interactors Detail

More details for 5 interactors identified by 3 citations

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5897_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5897_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	1700		90.1 (chain: 1, length: 172)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 172, Sequence coverage: 90.1%
- Total number of assignments
  - ¹H chemical shifts: 918
  - ¹³C chemical shifts: 639
  - ¹⁵N chemical shifts: 143
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1012	730	72.1
¹³C chemical shifts	784	639	81.5
¹⁵N chemical shifts	192	143	74.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	348	299	85.9
¹³C chemical shifts	344	286	83.1
¹⁵N chemical shifts	166	140	84.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	664	431	64.9
¹³C chemical shifts	440	353	80.2
¹⁵N chemical shifts	26	3	11.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	105	94	89.5
¹³C chemical shifts	105	93	88.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	99	69	69.7
¹³C chemical shifts	94	62	66.0
¹⁵N chemical shifts	5	3	60.0

Keywords Bcl-2, BHRF1, Epstein-Barr Virus, NMR spectroscopy, Structure determination

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Found 1 Document (1.645 seconds)

BMRB: 5897

Sample

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. homolog Bcl-2 Epstein Barr Virus, : 1 : 8 : 1 entities Detail

Interaction partners 1. homolog Bcl-2 Epstein Barr Virus, : 5 interactors Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 3 contents Detail