BMRB: 5903

1H,13C and 15N backbone resonance assignment of the Hyaluronan-binding domain of CD44

Authors

Takeda, M., Terasawa, H., Sakakura, M., Yamaguchi, Y., Kajiwara, M., Kawashima, H., Miyasaka, M., Shimada, I.

Assembly

CD44 hyaluronan-binding domain

Entity

1. CD44 hyaluronan-binding domain (polymer, Thiol state: all disulfide bound), 160 monomers, 17717.55 Da Detail

MAQIDLNITC RFAGVFHVEK NGRYSISRTE AADLCKAFNS TLPTMAQMEK ALSIGFETCR YGFIEGHVVI PRIHPNSICA ANNTGVYILT SNTSQYDTYC FNASAPPEED CTSVTDLPNA FDGPITITIV NRDGTRYVQK GEYRTNPEDI YPSNPTDDDV

Formula weight

17717.55 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS10:SG	1:CYS111:SG
2	disulfide	sing	1:CYS35:SG	1:CYS100:SG
3	disulfide	sing	1:CYS59:SG	1:CYS79:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.0 %, Completeness: 38.2 %, Completeness (bb): 65.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	38.2 % (682 of 1787)	23.6 % (217 of 919)	45.4 % (319 of 702)	88.0 % (146 of 166)
Backbone	65.0 % (611 of 940)	50.8 % (162 of 319)	64.5 % (304 of 471)	96.7 % (145 of 150)
Sidechain	9.1 % (91 of 998)	9.2 % (55 of 600)	9.2 % (35 of 382)	6.3 % (1 of 16)
Aromatic	0.0 % (0 of 146)	0.0 % (0 of 73)	0.0 % (0 of 73)
Methyl	16.1 % (27 of 168)	16.7 % (14 of 84)	15.5 % (13 of 84)

1. CD44 hyaluronan-binding domain

MAQIDLNITC RFAGVFHVEK NGRYSISRTE AADLCKAFNS TLPTMAQMEK ALSIGFETCR YGFIEGHVVI PRIHPNSICA ANNTGVYILT SNTSQYDTYC FNASAPPEED CTSVTDLPNA FDGPITITIV NRDGTRYVQK GEYRTNPEDI YPSNPTDDDV

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	CD44 hyaluronan-binding domain	[U-98% 13C; U-98% 15N]		2.0 mM

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Calculated from 20 models in PDB: 2I83, Strand ID: A Detail

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Release date

2003-08-14

Citation

1H, 13C and 15N backbone resonance assignments of the hyaluronan-binding domain of CD44
Takeda, M., Terasawa, H., Sakakura, M., Yamaguchi, Y., Kajiwara, M., Kawashima, H., Miyasaka, M., Shimada, I.
J. Biomol. NMR (2004), 29, 97-98, PubMed 15017146 , DOI 10.1023/B:JNMR.0000019465.12250.e0 , Abstract

Related entities 1. CD44 hyaluronan-binding domain, : 1 : 2 : 4 : 39 entities Detail

Interaction partners 1. CD44 hyaluronan-binding domain, : 55 interactors Detail

Experiments performed 5 experiments Detail