Backbone 1HN and 15N Chemical Shift Assignments for Sac7d V30I
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 31.7 % (158 of 499) | 21.3 % (92 of 432) | 98.5 % (66 of 67) |
Backbone | 66.2 % (133 of 201) | 50.7 % (69 of 136) | 98.5 % (64 of 65) |
Sidechain | 8.4 % (25 of 298) | 7.8 % (23 of 296) | 100.0 % (2 of 2) |
Aromatic | 20.0 % (5 of 25) | 16.7 % (4 of 24) | 100.0 % (1 of 1) |
Methyl | 6.9 % (2 of 29) | 6.9 % (2 of 29) |
1. Sac7d
MVKVKFKYKG EEKEVDTSKI KKVWRVGKMI SFTYDDNGKT GRGAVSEKDA PKELLDMLAR AEREKKTemperature 303 (±1) K, pH 4.06 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Sac7d | [U-98% 15N] | 1.18 mM | |
2 | KCl | 0.3 M | ||
3 | H2O | 90 % | ||
4 | D2O | 10 % | ||
5 | DSS | 100 uM |