BMRB: 5909

Backbone 1HN and 15N Chemical Shift Assignments for Sso7d

Authors

Clark, A.T., McCrary, B.S., Edmondson, S.P., Shriver, J.W.

Assembly

Sso7d monomer

Entity

1. Sso7d monomer (polymer, Thiol state: not present), 64 monomers, 7278.469 Da Detail

MATVKFKYKG EEKQVDISKI KKVWRVGKMI SFTYDEGGGK TGRGAVSEKD APKELLQMLE KQKK

Formula weight

7278.469 Da

Source organism

Sulfolobus acidocaldarius

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.3 %, Completeness: 18.6 %, Completeness (bb): 34.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	18.6 % (147 of 792)	18.0 % (76 of 423)	3.0 % (9 of 302)	92.5 % (62 of 67)
Backbone	34.6 % (132 of 382)	48.5 % (65 of 134)	3.2 % (6 of 185)	96.8 % (61 of 63)
Sidechain	3.9 % (18 of 467)	4.2 % (12 of 289)	2.9 % (5 of 174)	25.0 % (1 of 4)
Aromatic	4.2 % (2 of 48)	4.2 % (1 of 24)	0.0 % (0 of 23)	100.0 % (1 of 1)
Methyl	0.0 % (0 of 56)	0.0 % (0 of 28)	0.0 % (0 of 28)

1. Sso7d

MATVKFKYKG EEKQVDISKI KKVWRVGKMI SFTYDEGGGK TGRGAVSEKD APKELLQMLE KQKK

Show sample condition

Sample

Temperature 303 (±1) K, pH 5.15 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Sso7d	[U-99% 13C; U-90% 15N]		0.58 mM
2	H2O			90 %
3	D2O			10 %
4	DSS			100 uM

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Calculated from 1 models in PDB: 1BBX, Strand ID: C, D Detail

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Release date

2003-10-05

Citation 1

Thermodynamics of Hydrophobic Core Packing in the Thermophile Proteins Sac7d and Sso7d
Clark, A.T., McCrary, B.S., Edmondson, S.P., Shriver, J.W.
J. Mol. Biol.

Show more 6 citaions

Citation 2

DNA binding proteins Sac7d and Sso7d from Sulfolobus
Edmondson, S.P., Shriver, J.W.
Methods. Enzymol. (2001), 334, 129-145, PubMed 11398456 , Abstract

Citation 3

Crystal structures of the chromosomal proteins Sso7d/Sac7d bound to DNA containing T-G mismatched base-pairs
Su, S., Gao, Y.G., Robinson, H., Liaw, Y.C., Edmondson, S.P., Shriver, J.W., Wang, A.H.
J. Mol. Biol. (2000), 303, 395-403, PubMed 11031116 , DOI 10.1006/jmbi.2000.4112 , Abstract

Citation 4

The crystal structure of the hyperthermophile chromosomal protein Sso7d bound to DNA
Gao, Y.G., Su, S.Y., Robinson, H., Padmanabhan, S., Lim, L., McCrary, B.S., Edmondson, S.P., Shriver, J.W., Wang, A.H.
Nat. Struct. Biol. (1998), 5, 782-786, PubMed 9731772 , DOI 10.1038/1822 , Abstract

Citation 5

The hyperthermophile chromosomal protein Sac7d sharply kinks DNA
Robinson, H., Gao, Y.G., McCrary, B.S., Edmondson, S.P., Shriver, J.W., Wang, A.H.
Nature (1998), 392, 202-205, PubMed 9515968 , DOI 10.1038/32455 , Abstract

Citation 6

Solution structure of the DNA-binding protein Sac7d from the hyperthermophile Sulfolobus acidocaldarius
Edmondson, S.P., Qiu, L., Shriver, J.W.
Biochemistry (1995), 34, 13289-13304, PubMed 7577913 , Abstract

Citation 7

Gene cloning, expression, and characterization of the Sac7 proteins from the hyperthermophile Sulfolobus acidocaldarius
McAfee, J.G., Edmondson, S.P., Datta, P.K., Shriver, J.W., Gupta, R.
Biochemistry (1995), 34, 10063-10077, PubMed 7632679 , Abstract

Hide non-primary 6 citaions

Entries sharing articles BMRB: 3, Swiss-Prot: 3 entries Detail

Related entities 1. Sso7d monomer, : 1 : 1 : 19 : 6 : 135 entities Detail

Experiments performed 3 experiments Detail