BMRB: 5924

NMR Solution Structure of the Focal Adhesion Targeting Domain (FAT) of Focal Adhesion Kinase (FAK) in Complex with a Paxillin LD Peptide: Evidence for a Two Site Binding Model

Authors

Gao, G., Prutzman, K.C., King, M.L., DeRose, E.F., London, R.E., Schaller, M.D., Campbell, S.L.

Assembly

FAT domain of Focal adhesion kinase (920-1053)

Entity

1. FAT domain of Focal adhesion kinase (920-1053) (polymer, Thiol state: not present), 134 monomers, 14981.32 Da Detail

RSNDKVYENV TGLVKAVIEM SSKIQPAPPE EYVPMVKEVG LALRTLLATV DESLPVLPAS THREIEMAQK LLNSDLAELI NKMKLAQQYV MTSLQQEYKK QMLTAAHALA VDAKNLLDVI DQARLKMISQ SRPH

Formula weight

14981.32 Da

Source organism

Gallus gallus

Exptl. method

NMR

Refine. method

The program CNS version 1.1 with ARIA version 1.2 module was used to calculate the structures of the avian FAT domain.

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.5 %, Completeness: 71.2 %, Completeness (bb): 74.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	71.2 % (1125 of 1580)	75.3 % (621 of 825)	61.1 % (375 of 614)	91.5 % (129 of 141)
Backbone	74.2 % (586 of 790)	86.3 % (227 of 263)	60.0 % (240 of 400)	93.7 % (119 of 127)
Sidechain	69.2 % (638 of 922)	70.1 % (394 of 562)	67.6 % (234 of 346)	71.4 % (10 of 14)
Aromatic	90.9 % (40 of 44)	90.9 % (20 of 22)	90.9 % (20 of 22)
Methyl	80.4 % (148 of 184)	85.9 % (79 of 92)	75.0 % (69 of 92)

1. FAT domain of Focal adhesion kinase (920-1053)

RSNDKVYENV TGLVKAVIEM SSKIQPAPPE EYVPMVKEVG LALRTLLATV DESLPVLPAS THREIEMAQK LLNSDLAELI NKMKLAQQYV MTSLQQEYKK QMLTAAHALA VDAKNLLDVI DQARLKMISQ SRPH

Show sample condition

Sample

Temperature 310 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	FAT domain of Focal adhesion kinase (920-1053)	[U-13C; U-15N]		0.6 mM

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Protein Blocks Logo

Calculated from 25 models in PDB: 1QVX, Strand ID: A Detail

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Release date

2004-03-01

Citation

NMR solution structure of the focal adhesion targeting domain of focal adhesion kinase in complex with a paxillin LD peptide: evidence for a two-site binding model
Gao, G., Prutzman, K.C., King, M.L., Scheswohl, D.M., DeRose, E.F., London, R.E., Schaller, M.D., Campbell, S.L.
J. Biol. Chem. (2004), 279, 8441-8451, PubMed 14662767 , DOI 10.1074/jbc.M309808200 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. FAT domain of Focal adhesion kinase (920-1053), : 1 : 6 : 42 entities Detail

Interaction partners 1. FAT domain of Focal adhesion kinase (920-1053), : 6 interactors Detail

Experiments performed 14 experiments Detail