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BMRB: 5929

2DCQ

NMR assignment of the hypothetical rhodanase domain At4g01050 from Arabidopsis thaliana

Authors

Pantoja-Uceda, D., Lopez-Mendez, B., Koshiba, S., Kigawa, T., Shirouzu, M., Terada, T., Inoue, M., Yabuki, T., Aoki, M., Seki, E., Matsuda, T., Hirota, H., Yoshida, M., Tanaka, A., Osanai, T., Seki, M., Shinozaki, K., Yokoyama, S., Guntert, P.

Assembly

hypothetical rhodanase domain

Entity

1. hypothetical rhodanase domain (polymer, Thiol state: not present), 134 monomers, 14183.71 Da Detail

GSSGSSGSAK NAYTKLGTDD NAQLLDIRAT ADFRQVGSPN IKGLGKKAVS TVYNGEDKPG FLKKLSLKFK DPENTTLYIL DKFDGNSELV AELVALNGFK SAYAIKDGAE GPRGWLNSSL PWIEPKKTSG PSSG

Formula weight

14183.71 Da

Source organism

Arabidopsis thaliana

Exptl. method

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.5 %, Completeness (bb): 98.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.5 % (1456 of 1509)	97.3 % (765 of 786)	95.0 % (555 of 584)	97.8 % (136 of 139)
Backbone	98.2 % (776 of 790)	97.5 % (270 of 277)	99.0 % (382 of 386)	97.6 % (124 of 127)
Sidechain	95.2 % (797 of 837)	97.2 % (495 of 509)	91.8 % (290 of 316)	100.0 % (12 of 12)
Aromatic	76.4 % (81 of 106)	92.5 % (49 of 53)	58.8 % (30 of 51)	100.0 % (2 of 2)
Methyl	100.0 % (132 of 132)	100.0 % (66 of 66)	100.0 % (66 of 66)

1. single chain biopolymer

GSSGSSGSAK NAYTKLGTDD NAQLLDIRAT ADFRQVGSPN IKGLGKKAVS TVYNGEDKPG FLKKLSLKFK DPENTTLYIL DKFDGNSELV AELVALNGFK SAYAIKDGAE GPRGWLNSSL PWIEPKKTSG PSSG

Show sample condition

Sample

Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	single chain biopolymer	[U-13C; U-15N]		1.10 mM
2	phosphate buffer			20 mM
3	NaCl			100 mM
4	dithiothreitol			1 mM
5	sodium azide			0.02 % v/v
6	H2O			90 % v/v
7	D2O			10 % v/v

Protein Blocks Logo

Calculated from 20 models in PDB: 1VEE, Strand ID: A Detail

Release date

2004-04-06

Citation

NMR assignment of the hypothetical rhodanese domain At4g01050 from Arabidopsis thaliana
Pantoja-Uceda, D., Lopez-Mendez, B., Koshiba, S., Kigawa, T., Shirouzu, M., Terada, T., Inoue, M., Yabuki, T., Aoki, M., Seki, E., Matsuda, T., Hirota, H., Yoshida, M., Tanaka, A., Osanai, T., Seki, M., Shinozaki, K., Yokoyama, S., Guntert, P.
J. Biomol. NMR (2004), 29, 207-208, PubMed 15014235 , DOI 10.1023/B:JNMR.0000019241.66789.c3 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q9M158 released on 2005-08-16
Title STR4_ARATH Entity Rhodanese-like domain-containing protein 4, chloroplastic

Related entities 1. hypothetical rhodanase domain, : 1 : 1 : 2 : 18 entities Detail

Experiments performed 17 experiments Detail

1 [1H,15N ]-HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	single chain biopolymer	[U-13C; U-15N]		1.10 mM
2	phosphate buffer			20 mM
3	NaCl			100 mM
4	dithiothreitol			1 mM
5	sodium azide			0.02 % v/v
6	H2O			90 % v/v
7	D2O			10 % v/v

2 [1H,13C]-HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	single chain biopolymer	[U-13C; U-15N]		1.10 mM
2	phosphate buffer			20 mM
3	NaCl			100 mM
4	dithiothreitol			1 mM
5	sodium azide			0.02 % v/v
6	H2O			90 % v/v
7	D2O			10 % v/v

3 HNCO

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	single chain biopolymer	[U-13C; U-15N]		1.10 mM
2	phosphate buffer			20 mM
3	NaCl			100 mM
4	dithiothreitol			1 mM
5	sodium azide			0.02 % v/v
6	H2O			90 % v/v
7	D2O			10 % v/v

4 HN(CA)CO

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	single chain biopolymer	[U-13C; U-15N]		1.10 mM
2	phosphate buffer			20 mM
3	NaCl			100 mM
4	dithiothreitol			1 mM
5	sodium azide			0.02 % v/v
6	H2O			90 % v/v
7	D2O			10 % v/v

5 HNCA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	single chain biopolymer	[U-13C; U-15N]		1.10 mM
2	phosphate buffer			20 mM
3	NaCl			100 mM
4	dithiothreitol			1 mM
5	sodium azide			0.02 % v/v
6	H2O			90 % v/v
7	D2O			10 % v/v

6 HA-coupled HNCA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	single chain biopolymer	[U-13C; U-15N]		1.10 mM
2	phosphate buffer			20 mM
3	NaCl			100 mM
4	dithiothreitol			1 mM
5	sodium azide			0.02 % v/v
6	H2O			90 % v/v
7	D2O			10 % v/v

7 HN(CO)CA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	single chain biopolymer	[U-13C; U-15N]		1.10 mM
2	phosphate buffer			20 mM
3	NaCl			100 mM
4	dithiothreitol			1 mM
5	sodium azide			0.02 % v/v
6	H2O			90 % v/v
7	D2O			10 % v/v

8 HNCACB

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	single chain biopolymer	[U-13C; U-15N]		1.10 mM
2	phosphate buffer			20 mM
3	NaCl			100 mM
4	dithiothreitol			1 mM
5	sodium azide			0.02 % v/v
6	H2O			90 % v/v
7	D2O			10 % v/v

9 CBCA(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	single chain biopolymer	[U-13C; U-15N]		1.10 mM
2	phosphate buffer			20 mM
3	NaCl			100 mM
4	dithiothreitol			1 mM
5	sodium azide			0.02 % v/v
6	H2O			90 % v/v
7	D2O			10 % v/v

10 HNHA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	single chain biopolymer	[U-13C; U-15N]		1.10 mM
2	phosphate buffer			20 mM
3	NaCl			100 mM
4	dithiothreitol			1 mM
5	sodium azide			0.02 % v/v
6	H2O			90 % v/v
7	D2O			10 % v/v

11 HBHA(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	single chain biopolymer	[U-13C; U-15N]		1.10 mM
2	phosphate buffer			20 mM
3	NaCl			100 mM
4	dithiothreitol			1 mM
5	sodium azide			0.02 % v/v
6	H2O			90 % v/v
7	D2O			10 % v/v

12 (H)CC(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	single chain biopolymer	[U-13C; U-15N]		1.10 mM
2	phosphate buffer			20 mM
3	NaCl			100 mM
4	dithiothreitol			1 mM
5	sodium azide			0.02 % v/v
6	H2O			90 % v/v
7	D2O			10 % v/v

13 HCCH-COSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	single chain biopolymer	[U-13C; U-15N]		1.10 mM
2	phosphate buffer			20 mM
3	NaCl			100 mM
4	dithiothreitol			1 mM
5	sodium azide			0.02 % v/v
6	H2O			90 % v/v
7	D2O			10 % v/v

14 HCCH-TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	single chain biopolymer	[U-13C; U-15N]		1.10 mM
2	phosphate buffer			20 mM
3	NaCl			100 mM
4	dithiothreitol			1 mM
5	sodium azide			0.02 % v/v
6	H2O			90 % v/v
7	D2O			10 % v/v

15 H(CCCO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	single chain biopolymer	[U-13C; U-15N]		1.10 mM
2	phosphate buffer			20 mM
3	NaCl			100 mM
4	dithiothreitol			1 mM
5	sodium azide			0.02 % v/v
6	H2O			90 % v/v
7	D2O			10 % v/v

16 3D 15N-edited [1H,1H]-NOESY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	single chain biopolymer	[U-13C; U-15N]		1.10 mM
2	phosphate buffer			20 mM
3	NaCl			100 mM
4	dithiothreitol			1 mM
5	sodium azide			0.02 % v/v
6	H2O			90 % v/v
7	D2O			10 % v/v

17 13C-edited [1H,1H]-NOESY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	single chain biopolymer	[U-13C; U-15N]		1.10 mM
2	phosphate buffer			20 mM
3	NaCl			100 mM
4	dithiothreitol			1 mM
5	sodium azide			0.02 % v/v
6	H2O			90 % v/v
7	D2O			10 % v/v

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