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NMR assignment of the hypothetical rhodanase domain At4g01050 from Arabidopsis thaliana
Authors
Pantoja-Uceda, D., Lopez-Mendez, B., Koshiba, S., Kigawa, T., Shirouzu, M., Terada, T., Inoue, M., Yabuki, T., Aoki, M., Seki, E., Matsuda, T., Hirota, H., Yoshida, M., Tanaka, A., Osanai, T., Seki, M., Shinozaki, K., Yokoyama, S., Guntert, P.
Assembly
hypothetical rhodanase domain
Entity
1. hypothetical rhodanase domain (polymer, Thiol state: not present), 134 monomers, 14183.71 Da Detail

GSSGSSGSAK NAYTKLGTDD NAQLLDIRAT ADFRQVGSPN IKGLGKKAVS TVYNGEDKPG FLKKLSLKFK DPENTTLYIL DKFDGNSELV AELVALNGFK SAYAIKDGAE GPRGWLNSSL PWIEPKKTSG PSSG


Formula weight
14183.71 Da
Source organism
Arabidopsis thaliana
Exptl. method
NMR
Refine. method
TORSION ANGLE DYNAMICS
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 97.2 %, Completeness (bb): 99.5 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All97.2 % (1469 of 1512)98.1 % (771 of 786)95.7 % (559 of 584)97.9 % (139 of 142)
Backbone99.5 % (770 of 774)99.2 % (259 of 261)99.7 % (385 of 386)99.2 % (126 of 127)
Sidechain95.4 % (817 of 856)97.5 % (512 of 525)92.4 % (292 of 316)86.7 % (13 of 15)
Aromatic76.4 % (81 of 106)92.5 % (49 of 53)58.8 % (30 of 51)100.0 % (2 of 2)
Methyl100.0 % (132 of 132)100.0 % (66 of 66)100.0 % (66 of 66)

1. single chain biopolymer

GSSGSSGSAK NAYTKLGTDD NAQLLDIRAT ADFRQVGSPN IKGLGKKAVS TVYNGEDKPG FLKKLSLKFK DPENTTLYIL DKFDGNSELV AELVALNGFK SAYAIKDGAE GPRGWLNSSL PWIEPKKTSG PSSG

Sample

Temperature 298 (±0.5) K, pH 6.0 (±0.2)


#NameIsotope labelingTypeConcentration
1single chain biopolymer[U-13C; U-15N]1.10 mM
2phosphate buffer20 mM
3NaCl100 mM
4dithiothreitol1 mM
5sodium azide0.02 % v/v
6H2O90 % v/v
7D2O10 % v/v

Protein Blocks Logo
Calculated from 20 models in PDB: 1VEE, Strand ID: A Detail


Release date
2004-04-06
Citation
NMR assignment of the hypothetical rhodanese domain At4g01050 from Arabidopsis thaliana
Pantoja-Uceda, D., Lopez-Mendez, B., Koshiba, S., Kigawa, T., Shirouzu, M., Terada, T., Inoue, M., Yabuki, T., Aoki, M., Seki, E., Matsuda, T., Hirota, H., Yoshida, M., Tanaka, A., Osanai, T., Seki, M., Shinozaki, K., Yokoyama, S., Guntert, P.
J. Biomol. NMR (2004), 29, 207-208, PubMed 15014235 , DOI 10.1023/B:JNMR.0000019241.66789.c3 ,
Entries sharing articles Swiss-Prot: 1 entries Detail
  Swiss-Prot: Q9M158 released on 2005-08-16
    Title STR4_ARATH Entity Rhodanese-like domain-containing protein 4, chloroplastic
Related entities 1. hypothetical rhodanase domain, : 1 : 1 : 1 : 7 entities Detail
Experiments performed 17 experiments Detail