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Dynamics of the RNase H Domain of HIV-1 Reverse Transcriptase in the Presence of Magnesium and AMP
Authors
Pari, K., Mueller, G.A., DeRose, E.F., Kirby, T.W., London, R.E.
Assembly
RNase H domain of the HIV-1 reverse transcriptase
Entity
1. HIV-1 RNase H (polymer, Thiol state: not present), 138 monomers, 15260.14 × 3 Da Detail

MNELYQLEKE PIVGAETFYV DGAANRETKL GKAGYVTNRG RQKVVTLTDT TNQKTELQAI YLALQDSGLE VNIVTDSQYA LGIIQAQPDQ SESELVNQII EQLIKKEKVY LAWVPAHKGI GGNEQVDKLV SAGIRKVL


Total weight
45780.418 Da
Max. entity weight
15260.14 Da
Source organism
HIV-1 M:B_HXB2R
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.6 %, Completeness: 92.5 %, Completeness (bb): 95.3 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All92.5 % (1493 of 1614)92.8 % (776 of 836)91.9 % (569 of 619)93.1 % (148 of 159)
Backbone95.3 % (773 of 811)95.6 % (261 of 273)95.0 % (383 of 403)95.6 % (129 of 135)
Sidechain90.5 % (842 of 930)91.5 % (515 of 563)89.8 % (308 of 343)79.2 % (19 of 24)
Aromatic55.4 % (41 of 74)56.8 % (21 of 37)52.8 % (19 of 36)100.0 % (1 of 1)
Methyl94.7 % (178 of 188)95.7 % (90 of 94)93.6 % (88 of 94)

1. HIV-1 RNase H

MNELYQLEKE PIVGAETFYV DGAANRETKL GKAGYVTNRG RQKVVTLTDT TNQKTELQAI YLALQDSGLE VNIVTDSQYA LGIIQAQPDQ SESELVNQII EQLIKKEKVY LAWVPAHKGI GGNEQVDKLV SAGIRKVL

Sample

Temperature 298 (±0.25) K, pH 6.8 (±0.01)


#NameIsotope labelingTypeConcentration
1HIV-1 RNase H1.1 mM

LACS Plot; CA
Referencing offset: -0.12 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.12 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.09 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: 0.11 ppm, Outliers: 3 Detail
Release date
2004-04-06
Citation
Solution structure of the RNase H domain of the HIV-1 reverse transcriptase in the presence of magnesium
Pari, K., Mueller, G.A., DeRose, E.F., Kirby, T.W., London, R.E.
Biochemistry (2003), 42, 639-650, PubMed 12534276 , DOI 10.1021/bi0204894 ,
Related entities 1. HIV-1 RNase H, : 1 : 1 : 94 : 98 entities Detail
Interaction partners 1. HIV-1 RNase H, : 5 interactors Detail
Experiments performed 10 experiments Detail
Keywords HIV, Reverse Transcription, RNase H, NMR, Relaxation