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BMRB: 5960

2H3Q

1H, 13C, 15N- Chemical Shift Assignments for Myristoylated HIV-1 Matrix Protein

Authors

Tang, C., Loeliger, E., Luncsford, P., Kinde, I., Beckett, D., Summers, M.F.

Assembly

myristoylated HIV-1 matrix

Entity

1. myristoylated HIV-1 matrix (polymer, Thiol state: all free), 132 monomers, 14921.78 Da Detail

XGARASVLSG GELDKWEKIR LRPGGKKQYK LKHIVWASRE LERFAVNPGL LETSEGCRQI LGQLQPSLQT GSEELRSLYN TIAVLYCVHQ RIDVKDTKEA LDKIEEEQNK SKKKAQQAAA DTGNNSQVSQ NY

Formula weight

14921.78 Da

Source organism

Human immunodeficiency virus 1

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 78.9 %, Completeness (bb): 95.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	78.9 % (1218 of 1543)	72.6 % (592 of 815)	85.4 % (496 of 581)	88.4 % (130 of 147)
Backbone	95.5 % (745 of 780)	97.4 % (262 of 269)	93.0 % (356 of 383)	99.2 % (127 of 128)
Sidechain	64.7 % (572 of 884)	60.4 % (330 of 546)	74.9 % (239 of 319)	15.8 % (3 of 19)
Aromatic	45.9 % (34 of 74)	45.9 % (17 of 37)	42.9 % (15 of 35)	100.0 % (2 of 2)
Methyl	93.5 % (129 of 138)	94.2 % (65 of 69)	92.8 % (64 of 69)

1. p17

XGARASVLSG GELDKWEKIR LRPGGKKQYK LKHIVWASRE LERFAVNPGL LETSEGCRQI LGQLQPSLQT GSEELRSLYN TIAVLYCVHQ RIDVKDTKEA LDKIEEEQNK SKKKAQQAAA DTGNNSQVSQ NY

Show sample condition

Sample

Temperature 308 (±0.5) K, pH 5.5 (±0.1), Details 1x protease inhibitor cocktail set I (Calbiochem)

#	Name	Isotope labeling	Concentration
1	p17	[U-13C; U-15N]	0.2 mM
2	sodium phosophate buffer		50 mM
3	NaCl		50 mM
4	DTT		5 mM
5	DTT		5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	proton	4.677408 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 0.18 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	proton	4.677408 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 0.18 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	proton	4.677408 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	proton	4.677408 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: -0.14 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2H3Q, Strand ID: A Detail

Release date

2007-03-22

Citation

Entropic switch regulates myristate exposure in the HIV-1 matrix protein
Tang, C., Loeliger, E., Luncsford, P., Kinde, I., Beckett, D., Summers, M.F.
Proc. Natl. Acad. Sci. U. S. A. (2004), 101, 517-522, PubMed 14699046 , DOI 10.1073/pnas.0305665101 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P12497 released on 1989-10-01
Title POL_HV1N5 Entity Gag-Pol polyprotein

Related entities 1. myristoylated HIV-1 matrix, : 1 : 26 : 93 entities Detail

Interaction partners 1. myristoylated HIV-1 matrix, : 3 interactors Detail

More details for 3 interactors identified by 2 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC