BMRBj - BMREntryMaster

Found 1 Document (4.557 seconds)

BMRB: 5987

1R8U

Interaction of the TAZ1 domain of CREB-binding protein with the activation domain of CITED2: Regulation by competition between intrinsically unstructured ligands for non-identical binding sites

Authors

De Guzman, R.N., Martinez-Yamout, M., Dyson, H., Wright, P.E.

Assembly

CBP TAZ1 domain and CITED2 CAD

Entity

1. CITED2 C-terminal activation domain (polymer, Thiol state: all free), 51 monomers, 6024.886 Da Detail

MTDFIDEEVL MSLVIEMGLD RIKELPELWL GQNEFDFMTD FVCKQQPSRV S

2. CBP TAZ1 domain (polymer, Thiol state: all other bound), 100 monomers, 11256.98 Da Detail

ATGPTADPEK RKLIQQQLVL LLHAHKCQRR EQANGEVRAC SLPHCRTMKN VLNHMTHCQA GKACQVAHCA SSRQIISHWK NCTRHDCPVC LPLKNASDKR

3. ZN (non-polymer), 65.409 × 3 Da

Total weight

17478.094 Da

Max. entity weight

11256.98 Da

Entity Connection

na 12 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	2:HIS23:NE2	3:ZN1:ZN
2	na	sing	2:CYS27:SG	3:ZN1:ZN
3	na	sing	2:CYS40:SG	3:ZN1:ZN
4	na	sing	2:CYS45:SG	3:ZN1:ZN
5	na	sing	2:HIS54:NE2	3:ZN1:ZN
6	na	sing	2:CYS58:SG	3:ZN1:ZN
7	na	sing	2:CYS64:SG	3:ZN1:ZN
8	na	sing	2:CYS69:SG	3:ZN1:ZN
9	na	sing	2:HIS78:NE2	3:ZN1:ZN
10	na	sing	2:CYS82:SG	3:ZN1:ZN
11	na	sing	2:CYS87:SG	3:ZN1:ZN
12	na	sing	2:CYS90:SG	3:ZN1:ZN

Source organism

Homo sapiens , Mus musculus

Exptl. method

NMR

Refine. method

AMBER simulated annealing and minimization

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 39.7 %, Completeness: 31.2 %, Completeness (bb): 30.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	31.2 % (554 of 1773)	32.3 % (301 of 932)	28.9 % (196 of 678)	35.0 % (57 of 163)
Backbone	30.9 % (276 of 892)	36.3 % (109 of 300)	25.7 % (115 of 448)	36.1 % (52 of 144)
Sidechain	32.3 % (332 of 1027)	30.4 % (192 of 632)	35.9 % (135 of 376)	26.3 % (5 of 19)
Aromatic	25.0 % (24 of 96)	25.0 % (12 of 48)	23.9 % (11 of 46)	50.0 % (1 of 2)
Methyl	36.4 % (56 of 154)	35.1 % (27 of 77)	37.7 % (29 of 77)

1. CITED2 C-terminal activation domain

MTDFIDEEVL MSLVIEMGLD RIKELPELWL GQNEFDFMTD FVCKQQPSRV S

2. CBP TAZ1 domain

ATGPTADPEK RKLIQQQLVL LLHAHKCQRR EQANGEVRAC SLPHCRTMKN VLNHMTHCQA GKACQVAHCA SSRQIISHWK NCTRHDCPVC LPLKNASDKR

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	CITED2 C-terminal activation domain	[U-13C; U-15N]	0.5 mM
2	CBP TAZ1 domain		0.5 mM
3	Tris-d11		10 mM
4	NaN3		2 mM
5	D2O		10 %
6	H2O		90 %

Chem. Shift Complete2

Sequence coverage: 70.2 %, Completeness: 42.0 %, Completeness (bb): 43.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	42.0 % (1490 of 3546)	43.1 % (804 of 1864)	38.9 % (527 of 1356)	48.8 % (159 of 326)
Backbone	43.2 % (771 of 1784)	50.3 % (302 of 600)	36.4 % (326 of 896)	49.7 % (143 of 288)
Sidechain	42.4 % (871 of 2054)	39.7 % (502 of 1264)	46.9 % (353 of 752)	42.1 % (16 of 38)
Aromatic	33.3 % (64 of 192)	33.3 % (32 of 96)	32.6 % (30 of 92)	50.0 % (2 of 4)
Methyl	50.0 % (154 of 308)	49.4 % (76 of 154)	50.6 % (78 of 154)

1. CITED2 C-terminal activation domain

MTDFIDEEVL MSLVIEMGLD RIKELPELWL GQNEFDFMTD FVCKQQPSRV S

2. CBP TAZ1 domain

ATGPTADPEK RKLIQQQLVL LLHAHKCQRR EQANGEVRAC SLPHCRTMKN VLNHMTHCQA GKACQVAHCA SSRQIISHWK NCTRHDCPVC LPLKNASDKR

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
7	CITED2 C-terminal activation domain		0.5 mM
8	CBP TAZ1 domain	[U-13C; U-15N]	0.5 mM
9	Tris-d11		10 mM
10	NaN3		2 mM
11	D2O		10 %
12	H2O		90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.02 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1R8U, Strand ID: A, B Detail

Release date

2004-02-12

Citation

Interaction of the TAZ1 domain of the CREB-binding protein with the activation domain of CITED2: regulation by competition between intrinsically unstructured ligands for non-identical binding sites
De Guzman, R.N., Martinez-Yamout, M., Dyson, H., Wright, P.E.
J. Biol. Chem. (2004), 279, 3042-3049, PubMed 14594809 , DOI 10.1074/jbc.M310348200 , Abstract

Entries sharing articles Swiss-Prot: 2 entries Detail

  Swiss-Prot: Q99967 released on 1998-12-15
    Title CITE2_HUMAN Entity Cbp/p300-interacting transactivator 2
  Swiss-Prot: P45481 released on 1995-11-01
    Title CBP_MOUSE Entity CREB-binding protein

Related entities 1. CITED2 C-terminal activation domain, : 1 : 8 : 7 entities Detail

Related entities 2. CBP TAZ1 domain, : 6 : 47 entities Detail

Interaction partners 1. CITED2 C-terminal activation domain, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Interaction partners 2. CBP TAZ1 domain, : 78 interactors Detail

More details for 78 interactors identified by 53 citations

Experiments performed 9 experiments Detail

1 3D HNCA

Instrument

#1 Model Bruker AVANCE (900 MHz)

#2 Model Bruker AVANCE (800 MHz)

#3 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	CITED2 C-terminal activation domain	[U-13C; U-15N]	0.5 mM
2	CBP TAZ1 domain		0.5 mM
3	Tris-d11		10 mM
4	NaN3		2 mM
5	D2O		10 %
6	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
7	CITED2 C-terminal activation domain		0.5 mM
8	CBP TAZ1 domain	[U-13C; U-15N]	0.5 mM
9	Tris-d11		10 mM
10	NaN3		2 mM
11	D2O		10 %
12	H2O		90 %

2 3D CBCACONH

Instrument

#1 Model Bruker AVANCE (900 MHz)

#2 Model Bruker AVANCE (800 MHz)

#3 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	CITED2 C-terminal activation domain	[U-13C; U-15N]	0.5 mM
2	CBP TAZ1 domain		0.5 mM
3	Tris-d11		10 mM
4	NaN3		2 mM
5	D2O		10 %
6	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
7	CITED2 C-terminal activation domain		0.5 mM
8	CBP TAZ1 domain	[U-13C; U-15N]	0.5 mM
9	Tris-d11		10 mM
10	NaN3		2 mM
11	D2O		10 %
12	H2O		90 %

3 3D HNCACB

Instrument

#1 Model Bruker AVANCE (900 MHz)

#2 Model Bruker AVANCE (800 MHz)

#3 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	CITED2 C-terminal activation domain	[U-13C; U-15N]	0.5 mM
2	CBP TAZ1 domain		0.5 mM
3	Tris-d11		10 mM
4	NaN3		2 mM
5	D2O		10 %
6	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
7	CITED2 C-terminal activation domain		0.5 mM
8	CBP TAZ1 domain	[U-13C; U-15N]	0.5 mM
9	Tris-d11		10 mM
10	NaN3		2 mM
11	D2O		10 %
12	H2O		90 %

4 3D HCCH-TOCSY

Instrument

#1 Model Bruker AVANCE (900 MHz)

#2 Model Bruker AVANCE (800 MHz)

#3 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	CITED2 C-terminal activation domain	[U-13C; U-15N]	0.5 mM
2	CBP TAZ1 domain		0.5 mM
3	Tris-d11		10 mM
4	NaN3		2 mM
5	D2O		10 %
6	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
7	CITED2 C-terminal activation domain		0.5 mM
8	CBP TAZ1 domain	[U-13C; U-15N]	0.5 mM
9	Tris-d11		10 mM
10	NaN3		2 mM
11	D2O		10 %
12	H2O		90 %

5 3D HCCH-COSY

Instrument

#1 Model Bruker AVANCE (900 MHz)

#2 Model Bruker AVANCE (800 MHz)

#3 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	CITED2 C-terminal activation domain	[U-13C; U-15N]	0.5 mM
2	CBP TAZ1 domain		0.5 mM
3	Tris-d11		10 mM
4	NaN3		2 mM
5	D2O		10 %
6	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
7	CITED2 C-terminal activation domain		0.5 mM
8	CBP TAZ1 domain	[U-13C; U-15N]	0.5 mM
9	Tris-d11		10 mM
10	NaN3		2 mM
11	D2O		10 %
12	H2O		90 %

6 3D 1H-15N NOESY

Instrument

#1 Model Bruker AVANCE (900 MHz)

#2 Model Bruker AVANCE (800 MHz)

#3 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	CITED2 C-terminal activation domain	[U-13C; U-15N]	0.5 mM
2	CBP TAZ1 domain		0.5 mM
3	Tris-d11		10 mM
4	NaN3		2 mM
5	D2O		10 %
6	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
7	CITED2 C-terminal activation domain		0.5 mM
8	CBP TAZ1 domain	[U-13C; U-15N]	0.5 mM
9	Tris-d11		10 mM
10	NaN3		2 mM
11	D2O		10 %
12	H2O		90 %

7 3D 1H-13C NOESY

Instrument

#1 Model Bruker AVANCE (900 MHz)

#2 Model Bruker AVANCE (800 MHz)

#3 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	CITED2 C-terminal activation domain	[U-13C; U-15N]	0.5 mM
2	CBP TAZ1 domain		0.5 mM
3	Tris-d11		10 mM
4	NaN3		2 mM
5	D2O		10 %
6	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
7	CITED2 C-terminal activation domain		0.5 mM
8	CBP TAZ1 domain	[U-13C; U-15N]	0.5 mM
9	Tris-d11		10 mM
10	NaN3		2 mM
11	D2O		10 %
12	H2O		90 %

8 2D HSQC

Instrument

#1 Model Bruker AVANCE (900 MHz)

#2 Model Bruker AVANCE (800 MHz)

#3 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	CITED2 C-terminal activation domain	[U-13C; U-15N]	0.5 mM
2	CBP TAZ1 domain		0.5 mM
3	Tris-d11		10 mM
4	NaN3		2 mM
5	D2O		10 %
6	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
7	CITED2 C-terminal activation domain		0.5 mM
8	CBP TAZ1 domain	[U-13C; U-15N]	0.5 mM
9	Tris-d11		10 mM
10	NaN3		2 mM
11	D2O		10 %
12	H2O		90 %

9 2D 1H-13C HMQC

Instrument

#1 Model Bruker AVANCE (900 MHz)

#2 Model Bruker AVANCE (800 MHz)

#3 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	CITED2 C-terminal activation domain	[U-13C; U-15N]	0.5 mM
2	CBP TAZ1 domain		0.5 mM
3	Tris-d11		10 mM
4	NaN3		2 mM
5	D2O		10 %
6	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
7	CITED2 C-terminal activation domain		0.5 mM
8	CBP TAZ1 domain	[U-13C; U-15N]	0.5 mM
9	Tris-d11		10 mM
10	NaN3		2 mM
11	D2O		10 %
12	H2O		90 %

nullKeywords CBP, TAZ1, CITED2, NMR

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Found 1 Document (4.557 seconds)

BMRB: 5987

Sample

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 2 entries Detail

Related entities 1. CITED2 C-terminal activation domain, : 1 : 8 : 7 entities Detail

Related entities 2. CBP TAZ1 domain, : 6 : 47 entities Detail

Interaction partners 1. CITED2 C-terminal activation domain, : 1 interactors Detail

Interaction partners 2. CBP TAZ1 domain, : 78 interactors Detail

Experiments performed 9 experiments Detail

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