BMRB: 6033

NMR characterisation shows that a b-type variant of Hydrogenobacter thermophilus cytochrome c552 retains its native fold

Authors

Wain, R., Redfield, C., Smith, L.J.

Assembly

C10A/C13A cytochrome c552 from Hydrogenobacter thermphilus

Entity

1. Cytochrome c552 from Hydrogenobacter thermophilus (polymer, Thiol state: not present), 80 monomers, 8506.764 Da Detail

NEQLAKQKGA MAAHDLKAKK VGPAYADVAK KYAGRKDAVD YLAGKIKKGG SGVWGSVPMP PQNVTDAEAK QLAQWILSIK

2. HEM (non-polymer), 616.487 Da

Total weight

9123.251 Da

Max. entity weight

8506.764 Da

Source organism

Hydrogenobacter thermophilus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.0 %, Completeness: 57.6 %, Completeness (bb): 98.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	57.6 % (327 of 568)	52.0 % (251 of 483)	89.4 % (76 of 85)
Backbone	98.3 % (236 of 240)	97.6 % (160 of 164)	100.0 % (76 of 76)
Sidechain	27.7 % (91 of 328)	28.5 % (91 of 319)	0.0 % (0 of 9)
Aromatic	0.0 % (0 of 28)	0.0 % (0 of 26)	0.0 % (0 of 2)
Methyl	32.6 % (14 of 43)	32.6 % (14 of 43)

1. Cytochrome c552 from Hydrogenobacter thermophilus

NEQLAKQKGA MAAHDLKAKK VGPAYADVAK KYAGRKDAVD YLAGKIKKGG SGVWGSVPMP PQNVTDAEAK QLAQWILSIK

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Sample

Temperature 298 (±0.5) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Cytochrome c552 from Hydrogenobacter thermophilus	[U-15N]		1.0 mM

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Release date

2004-02-11

Citation

NMR analysis shows that a b-type variant of Hydrogenobacter thermophilus cytochrome c552 retains its native structure
Wain, R., Redfield, C., Smith, L.J.
J. Biol. Chem. (2004), 279, 15177-15182, PubMed 14726539 , DOI 10.1074/jbc.M311869200 , Abstract

Related entities 1. Cytochrome c552 from Hydrogenobacter thermophilus, : 1 : 5 : 41 entities Detail

Experiments performed 4 experiments Detail

Chemical shift validation 4 contents Detail