BMRB: 6044

Resonance assignment and secondary structure of the La motif

Authors

Sanfelice, D., BaboN, J., Kelly, G., Curry, S., Conte, M.R.

Assembly

La protein

Entity

1. La protein (polymer, Thiol state: not reported), 103 monomers, 12003.65 Da Detail

MAENGDNEKM AALEAKICHQ IEYYFGDFNL PRDKFLKEQI KLDEGWVPLE IMIKFNRLNR LTTDFNVIVE ALSKSKAELM EISEDKTKIR RSPSKPLPEV TDE

Formula weight

12003.65 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 92.7 %, Completeness (bb): 95.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.7 % (1169 of 1261)	91.4 % (609 of 666)	95.1 % (464 of 488)	89.7 % (96 of 107)
Backbone	95.7 % (582 of 608)	95.1 % (194 of 204)	96.4 % (295 of 306)	94.9 % (93 of 98)
Sidechain	90.8 % (684 of 753)	89.8 % (415 of 462)	94.3 % (266 of 282)	33.3 % (3 of 9)
Aromatic	95.1 % (78 of 82)	95.1 % (39 of 41)	95.0 % (38 of 40)	100.0 % (1 of 1)
Methyl	99.1 % (107 of 108)	98.1 % (53 of 54)	100.0 % (54 of 54)

1. La protein

MAENGDNEKM AALEAKICHQ IEYYFGDFNL PRDKFLKEQI KLDEGWVPLE IMIKFNRLNR LTTDFNVIVE ALSKSKAELM EISEDKTKIR RSPSKPLPEV TDE

Show sample condition

Sample

Temperature 293 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	La protein	[U-15N; U-13C]		0.9 mM

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LACS Plot; CA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 1S7A, Strand ID: A Detail

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Release date

2004-06-29

Citation

Resonance assignment and secondary structure of the La motif
Sanfelice, D., Babon, J., Kelly, G., Curry, S., Conte, M.R.
J. Biomol. NMR (2004), 29, 449-450, PubMed 15213463 , DOI 10.1023/B:JNMR.0000032556.32985.89 , Abstract

Related entities 1. La protein, : 1 : 3 : 6 : 99 entities Detail

Interaction partners 1. La protein, : 84 interactors Detail