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BMRB: 6046

1S6N

1H, 13C and 15N resonance assignments for domain III of the West Nile Virus envelope protein

Authors

Volk, D.E., Kallick, D.A., Holbrook, M.R., Beasley, D.W.C., Barrett, A.D.T., Gorenstein, D.G.

Assembly

West Nile Virus I, strain 385-99, Domain III

Entity

1. West Nile Virus I, strain 385-99, Domain III (polymer, Thiol state: all disulfide bound), 115 monomers, 12184.70 Da Detail

ISEFQLKGTT YGVCSKAFKF LGTPADTGHG TVVLELQYTG TDGPCKVPIS SVASLNDLTP VGRLVTVNPF VSVATANAKV LIELEPPFGD SYIVVGRGEQ QINHHWHKSG SSIGK

Formula weight

12184.7 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS14:SG	1:CYS45:SG

Source organism

West Nile virus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.3 %, Completeness: 91.0 %, Completeness (bb): 95.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.0 % (1169 of 1285)	93.8 % (616 of 657)	86.3 % (441 of 511)	95.7 % (112 of 117)
Backbone	95.6 % (646 of 676)	96.6 % (228 of 236)	94.9 % (315 of 332)	95.4 % (103 of 108)
Sidechain	87.8 % (624 of 711)	92.4 % (389 of 421)	80.4 % (226 of 281)	100.0 % (9 of 9)
Aromatic	46.1 % (47 of 102)	64.7 % (33 of 51)	26.0 % (13 of 50)	100.0 % (1 of 1)
Methyl	95.1 % (137 of 144)	95.8 % (69 of 72)	94.4 % (68 of 72)

1. West Nile Virus envelope protein domain III

ISEFQLKGTT YGVCSKAFKF LGTPADTGHG TVVLELQYTG TDGPCKVPIS SVASLNDLTP VGRLVTVNPF VSVATANAKV LIELEPPFGD SYIVVGRGEQ QINHHWHKSG SSIGK

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	West Nile Virus envelope protein domain III	[U-95% 13C; U-95% 15N]	0.7 mM
2	K2HPO4		50 mM
3	NaCl		100 mM
4	NaN3		10 mM
5	EDTA		0.1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.5 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.5 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.52 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 1S6N, Strand ID: A Detail

Release date

2003-12-14

Citation

1H, 13C and 15N resonance assignments for domain III of the West Nile virus envelope protein
Volk, D.E., Kallick, D.A., Holbrook, M.R., Beasley, D.W.C., Barrett, A.D.T., Gorenstein, D.G.
J. Biomol. NMR (2004), 29, 445-446, PubMed 15213461 , DOI 10.1023/B:JNMR.0000032551.56061.68 , Abstract

Related entities 1. West Nile Virus I, strain 385-99, Domain III, : 1 : 3 : 174 entities Detail

Interaction partners 1. West Nile Virus I, strain 385-99, Domain III, : 37 interactors Detail

More details for 37 interactors identified by 1 citations

Experiments performed 8 experiments Detail

1 HNCA