BMRBj - BMREntryMaster

Found 1 Document (2.407 seconds)

BMRB: 6048

2QSS

NEW STRUCTURAL FAMILY OF AN ANTIMICROBIAL PEPTIDE DERIVED FROM BOVINE HEMOGLOBIN

Authors

Sforca, M.L., Figueredo, R.C.R., Machado, A., Oyama, S., Miranda, M.T.M., Miranda, A., Daffre, S., Spisni, A., Pertinhez, T.A.

Assembly

Hb3361a peptide

Entity

1. Hb3361a peptide (polymer, Thiol state: not present), 30 monomers, 3204.570 Da Detail

FLSFPTTKTY FPHFDLSHGS AQVKGHGAKX

Formula weight

3204.57 Da

Source organism

Rhipicephalus microplus

Exptl. method

NMR

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 96.7 %, Completeness: 85.8 %, Completeness (bb): 93.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	85.8 % (151 of 176)	85.8 % (151 of 176)
Backbone	93.2 % (55 of 59)	93.2 % (55 of 59)
Sidechain	82.1 % (96 of 117)	82.1 % (96 of 117)
Aromatic	80.0 % (24 of 30)	80.0 % (24 of 30)
Methyl	100.0 % (11 of 11)	100.0 % (11 of 11)

1. Hemoglobin fragment

FLSFPTTKTY FPHFDLSHGS AQVKGHGAKX

Show sample condition

Sample

Temperature 310 (±1) K, pH 4.0 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	Hemoglobin fragment			1 mM
2	SDS			200 mM

Coupling constant

22 J values in 1 lists
Temperature 310 (±1) K, pH 4.0 (±0.5) Detail

Release date

2008-07-15

Citation

The micelle-bound structure of an antimicrobial peptide derived from the alpha-chain of bovine hemoglobin isolated from the tick Boophilus microplus
Sforca, M.L., Machado, A., Figueredo, R.C.R., Oyama, S., Silva, F.D., Miranda, A., Daffre, S., Miranda, M.T.M., Spisni, A., Pertinhez, T.A.
Biochemistry (2005), 44, 6440-6451, PubMed 15850378 , DOI 10.1021/bi0475323 , Abstract

Entries sharing articles BMRB: 5 entries Detail

  BMRB: 6414 released on 2008-07-16
    Title FRAGMENT 33-61 OF BOVINE alpha-HEMOGLBIN: THE EFFECT OF C-TERMINAL AMIDATION AND IDENTIFICATION OF THE MINIMAL PORTION WITH ANTIFUNGAL ACTIVITY
  BMRB: 6360 released on 2008-07-15
    Title IDENTIFICATION OF MINIMAL PEPTIDE SEQUENCE IN THE AMIDATED FRAGMENT 33-61 OF BOVINE a-HEMOGLBIN
  BMRB: 6411 released on 2008-07-15
    Title FRAGMENT 33-61 OF BOVINE alpha-HEMOGLBIN: THE EFFECT OF C-TERMINAL AMIDATION AND IDENTIFICATION OF THE MINIMAL PORTION WITH ANTIFUNGAL ACTIVITY
  BMRB: 6412 released on 2005-10-19
    Title FRAGMENT 33-61 OF BOVINE alpha-HEMOGLBIN: THE EFFECT OF C-TERMINAL AMIDATION AND IDENTIFICATION OF THE MINIMAL PORTION WITH ANTIFUNGAL ACTIVITY
  BMRB: 6413 released on 2005-10-19
    Title FRAGMENT 33-61 OF BOVINE alpha-HEMOGLBIN: THE EFFECT OF C-TERMINAL AMIDATION AND IDENTIFICATION OF THE MINIMAL PORTION WITH ANTIFUNGAL ACTIVITY

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr6048_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	phem_chem_shift_1	Warning	178		100.0 (chain: 1, length: 30)
1	Chemical shift references	chemical_shift_reference	OK	1		No information

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
13	HIS	HE2	8.44
18	HIS	HE2	8.49
26	HIS	HE2	8.55

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Found 1 Document (2.407 seconds)

BMRB: 6048

Sample

Entries sharing articles BMRB: 5 entries Detail

Related entities 1. Hb3361a peptide, : 1 : 171 : 51 entities Detail

Interaction partners 1. Hb3361a peptide, : 32 interactors Detail

Experiments performed 3 experiments Detail

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Instrument

Sample

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Sample

Chemical shift validation 3 contents Detail