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NEW STRUCTURAL FAMILY OF AN ANTIMICROBIAL PEPTIDE DERIVED FROM BOVINE HEMOGLOBIN
Authors
Sforca, M.L., Figueredo, R.C.R., Machado, A., Oyama, S., Miranda, M.T.M., Miranda, A., Daffre, S., Spisni, A., Pertinhez, T.A.
Assembly
Hb3361a peptide
Entity
1. Hb3361a peptide (polymer, Thiol state: not present), 30 monomers, 3204.570 Da Detail

FLSFPTTKTY FPHFDLSHGS AQVKGHGAKX


Formula weight
3204.57 Da
Source organism
Rhipicephalus microplus
Exptl. method
NMR
Data set
assigned_chemical_shifts, coupling_constants
Chem. Shift Complete
Sequence coverage: 96.7 %, Completeness: 85.8 %, Completeness (bb): 92.9 % Detail

Polymer type: polypeptide(L)

Total1H
All85.8 % (151 of 176)85.8 % (151 of 176)
Backbone92.9 % (52 of 56)92.9 % (52 of 56)
Sidechain82.5 % (99 of 120)82.5 % (99 of 120)
Aromatic80.0 % (24 of 30)80.0 % (24 of 30)
Methyl100.0 % (11 of 11)100.0 % (11 of 11)

1. Hemoglobin fragment

FLSFPTTKTY FPHFDLSHGS AQVKGHGAKX

Sample

Temperature 310 (±1) K, pH 4.0 (±0.5)


#NameIsotope labelingTypeConcentration
1Hemoglobin fragment1 mM
2SDS200 mM

Coupling constant
22 J values in 1 lists
Temperature 310 (±1) K, pH 4.0 (±0.5) Detail
Release date
2008-07-15
Citation
The micelle-bound structure of an antimicrobial peptide derived from the alpha-chain of bovine hemoglobin isolated from the tick Boophilus microplus
Sforca, M.L., Machado, A., Figueredo, R.C.R., Oyama, S., Silva, F.D., Miranda, A., Daffre, S., Miranda, M.T.M., Spisni, A., Pertinhez, T.A.
Biochemistry (2005), 44, 6440-6451, PubMed 15850378 , DOI 10.1021/bi0475323 ,
Related entities 1. Hb3361a peptide, : 1 : 170 : 29 entities Detail
Interaction partners 1. Hb3361a peptide, : 30 interactors Detail
Experiments performed 3 experiments Detail