BMRBj - BMREntryMaster

Found 1 Document (1.293 seconds)

BMRB: 6071

1TMW

1H, 13C and 15N Resonance Assignments and Secondary Structure of Human Coactosin Like Protein D123N

Authors

Dai, H., Wu, J., Xu, Y., Shi, Y.

Assembly

Coactosin like protein

Entity

1. Coactosin like protein (polymer, Thiol state: all free), 150 monomers, 17008.90 Da Detail

MATKIDKEAC RAAYNLVRDD GSAVIWVTFK YDGSTIVPGE QGAEYQHFIQ QCTDDVRLFA FVRFTTGDAM SKRSKFALIT WIGENVSGLQ RAKTGTDKTL VKEVVQNFAK EFVISDRKEL EENFIKSELK KAGGANYDAQ TELEHHHHHH

Formula weight

17008.9 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.3 %, Completeness: 85.1 %, Completeness (bb): 94.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.1 % (1492 of 1753)	84.7 % (767 of 906)	83.3 % (570 of 684)	95.1 % (155 of 163)
Backbone	94.0 % (844 of 898)	92.9 % (287 of 309)	94.5 % (416 of 440)	94.6 % (141 of 149)
Sidechain	78.4 % (780 of 995)	80.4 % (480 of 597)	74.5 % (286 of 384)	100.0 % (14 of 14)
Aromatic	2.3 % (4 of 174)	2.3 % (2 of 87)	0.0 % (0 of 85)	100.0 % (2 of 2)
Methyl	98.7 % (156 of 158)	97.5 % (77 of 79)	100.0 % (79 of 79)

1. coactosin like protein

MATKIDKEAC RAAYNLVRDD GSAVIWVTFK YDGSTIVPGE QGAEYQHFIQ QCTDDVRLFA FVRFTTGDAM SKRSKFALIT WIGENVSGLQ RAKTGTDKTL VKEVVQNFAK EFVISDRKEL EENFIKSELK KAGGANYDAQ TELEHHHHHH

Show sample condition

Sample #1

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	coactosin like protein	[U-99% 15N]	1.4 ~ 1.6 mM
2	PBS buffer		50 mM
3	NaCl		50 mM
4	D2O		10 %

Sample #2

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	coactosin like protein	[U-99% 13C; U-99% 15N]	1.4 ~ 1.6 mM
6	PBS buffer		50 mM
7	NaCl		50 mM
8	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.792 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.792 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.792 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.792 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 0.25 ppm, Outliers: 7 Detail

Release date

2004-07-05

Citation

1H, 13C and 15N resonance assignments and the secondary structures of human coactosin like protein (hCLP) D123N
Dai, H., Wu, J., Xu, Y., Yajun, T., Husheng, D., Shi, Y.
J. Biomol. NMR (2004), 29, 455-456, PubMed 15213466 , DOI 10.1023/B:JNMR.0000032550.18424.aa , Abstract

Related entities 1. Coactosin like protein, : 1 : 36 entities Detail

Interaction partners 1. Coactosin like protein, : 7 interactors Detail

More details for 7 interactors identified by 4 citations

Experiments performed 15 experiments Detail

1 2D 1H,15N-HSQC

Instrument

#1 Model Bruker AV500 (500 MHz)

#2 Model Bruker AV500 (600 MHz)

Sample #1

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	coactosin like protein	[U-99% 15N]	1.4 ~ 1.6 mM
2	PBS buffer		50 mM
3	NaCl		50 mM
4	D2O		10 %

Sample #2

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	coactosin like protein	[U-99% 13C; U-99% 15N]	1.4 ~ 1.6 mM
6	PBS buffer		50 mM
7	NaCl		50 mM
8	D2O		10 %

2 3D 15N-edited TOCSY-HSQC

Instrument

#1 Model Bruker AV500 (500 MHz)

#2 Model Bruker AV500 (600 MHz)

Sample #1

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	coactosin like protein	[U-99% 15N]	1.4 ~ 1.6 mM
2	PBS buffer		50 mM
3	NaCl		50 mM
4	D2O		10 %

Sample #2

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	coactosin like protein	[U-99% 13C; U-99% 15N]	1.4 ~ 1.6 mM
6	PBS buffer		50 mM
7	NaCl		50 mM
8	D2O		10 %

3 3D 15N-edited NOESY-HSQC

Instrument

#1 Model Bruker AV500 (500 MHz)

#2 Model Bruker AV500 (600 MHz)

Sample #1

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	coactosin like protein	[U-99% 15N]	1.4 ~ 1.6 mM
2	PBS buffer		50 mM
3	NaCl		50 mM
4	D2O		10 %

Sample #2

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	coactosin like protein	[U-99% 13C; U-99% 15N]	1.4 ~ 1.6 mM
6	PBS buffer		50 mM
7	NaCl		50 mM
8	D2O		10 %

4 3D HNCO

Instrument

#1 Model Bruker AV500 (500 MHz)

#2 Model Bruker AV500 (600 MHz)

Sample #1

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	coactosin like protein	[U-99% 15N]	1.4 ~ 1.6 mM
2	PBS buffer		50 mM
3	NaCl		50 mM
4	D2O		10 %

Sample #2

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	coactosin like protein	[U-99% 13C; U-99% 15N]	1.4 ~ 1.6 mM
6	PBS buffer		50 mM
7	NaCl		50 mM
8	D2O		10 %

5 3D HNCACO

Instrument

#1 Model Bruker AV500 (500 MHz)

#2 Model Bruker AV500 (600 MHz)

Sample #1

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	coactosin like protein	[U-99% 15N]	1.4 ~ 1.6 mM
2	PBS buffer		50 mM
3	NaCl		50 mM
4	D2O		10 %

Sample #2

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	coactosin like protein	[U-99% 13C; U-99% 15N]	1.4 ~ 1.6 mM
6	PBS buffer		50 mM
7	NaCl		50 mM
8	D2O		10 %

6 3D HNCA

Instrument

#1 Model Bruker AV500 (500 MHz)

#2 Model Bruker AV500 (600 MHz)

Sample #1

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	coactosin like protein	[U-99% 15N]	1.4 ~ 1.6 mM
2	PBS buffer		50 mM
3	NaCl		50 mM
4	D2O		10 %

Sample #2

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	coactosin like protein	[U-99% 13C; U-99% 15N]	1.4 ~ 1.6 mM
6	PBS buffer		50 mM
7	NaCl		50 mM
8	D2O		10 %

7 3D HNCOCA

Instrument

#1 Model Bruker AV500 (500 MHz)

#2 Model Bruker AV500 (600 MHz)

Sample #1

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	coactosin like protein	[U-99% 15N]	1.4 ~ 1.6 mM
2	PBS buffer		50 mM
3	NaCl		50 mM
4	D2O		10 %

Sample #2

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	coactosin like protein	[U-99% 13C; U-99% 15N]	1.4 ~ 1.6 mM
6	PBS buffer		50 mM
7	NaCl		50 mM
8	D2O		10 %

8 3D CBCA(CO)NH

Instrument

#1 Model Bruker AV500 (500 MHz)

#2 Model Bruker AV500 (600 MHz)

Sample #1

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	coactosin like protein	[U-99% 15N]	1.4 ~ 1.6 mM
2	PBS buffer		50 mM
3	NaCl		50 mM
4	D2O		10 %

Sample #2

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	coactosin like protein	[U-99% 13C; U-99% 15N]	1.4 ~ 1.6 mM
6	PBS buffer		50 mM
7	NaCl		50 mM
8	D2O		10 %

9 3D CBCANH

Instrument

#1 Model Bruker AV500 (500 MHz)

#2 Model Bruker AV500 (600 MHz)

Sample #1

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	coactosin like protein	[U-99% 15N]	1.4 ~ 1.6 mM
2	PBS buffer		50 mM
3	NaCl		50 mM
4	D2O		10 %

Sample #2

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	coactosin like protein	[U-99% 13C; U-99% 15N]	1.4 ~ 1.6 mM
6	PBS buffer		50 mM
7	NaCl		50 mM
8	D2O		10 %

10 3D H(C)(CO)NH-TOCSY

Instrument

#1 Model Bruker AV500 (500 MHz)

#2 Model Bruker AV500 (600 MHz)

Sample #1

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	coactosin like protein	[U-99% 15N]	1.4 ~ 1.6 mM
2	PBS buffer		50 mM
3	NaCl		50 mM
4	D2O		10 %

Sample #2

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	coactosin like protein	[U-99% 13C; U-99% 15N]	1.4 ~ 1.6 mM
6	PBS buffer		50 mM
7	NaCl		50 mM
8	D2O		10 %

11 3D (H)C(CO)NH-TOCSY

Instrument

#1 Model Bruker AV500 (500 MHz)

#2 Model Bruker AV500 (600 MHz)

Sample #1

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	coactosin like protein	[U-99% 15N]	1.4 ~ 1.6 mM
2	PBS buffer		50 mM
3	NaCl		50 mM
4	D2O		10 %

Sample #2

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	coactosin like protein	[U-99% 13C; U-99% 15N]	1.4 ~ 1.6 mM
6	PBS buffer		50 mM
7	NaCl		50 mM
8	D2O		10 %

12 3D HBHA(CBCACO)NH

Instrument

#1 Model Bruker AV500 (500 MHz)

#2 Model Bruker AV500 (600 MHz)

Sample #1

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	coactosin like protein	[U-99% 15N]	1.4 ~ 1.6 mM
2	PBS buffer		50 mM
3	NaCl		50 mM
4	D2O		10 %

Sample #2

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	coactosin like protein	[U-99% 13C; U-99% 15N]	1.4 ~ 1.6 mM
6	PBS buffer		50 mM
7	NaCl		50 mM
8	D2O		10 %

13 3D HCCH-TOCSY

Instrument

#1 Model Bruker AV500 (500 MHz)

#2 Model Bruker AV500 (600 MHz)

Sample #1

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	coactosin like protein	[U-99% 15N]	1.4 ~ 1.6 mM
2	PBS buffer		50 mM
3	NaCl		50 mM
4	D2O		10 %

Sample #2

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	coactosin like protein	[U-99% 13C; U-99% 15N]	1.4 ~ 1.6 mM
6	PBS buffer		50 mM
7	NaCl		50 mM
8	D2O		10 %

14 3D HCCH-COSY

Instrument

#1 Model Bruker AV500 (500 MHz)

#2 Model Bruker AV500 (600 MHz)

Sample #1

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	coactosin like protein	[U-99% 15N]	1.4 ~ 1.6 mM
2	PBS buffer		50 mM
3	NaCl		50 mM
4	D2O		10 %

Sample #2

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	coactosin like protein	[U-99% 13C; U-99% 15N]	1.4 ~ 1.6 mM
6	PBS buffer		50 mM
7	NaCl		50 mM
8	D2O		10 %

15 13C-edited NOESY-HSQC

Instrument

#1 Model Bruker AV500 (500 MHz)

#2 Model Bruker AV500 (600 MHz)

Sample #1

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	coactosin like protein	[U-99% 15N]	1.4 ~ 1.6 mM
2	PBS buffer		50 mM
3	NaCl		50 mM
4	D2O		10 %

Sample #2

Temperature 296 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	coactosin like protein	[U-99% 13C; U-99% 15N]	1.4 ~ 1.6 mM
6	PBS buffer		50 mM
7	NaCl		50 mM
8	D2O		10 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr6071_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr6071_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	1685		95.3 (chain: 1, length: 150)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 150, Sequence coverage: 95.3%
- Total number of assignments
  - ¹H chemical shifts: 939
  - ¹³C chemical shifts: 584
  - ¹⁵N chemical shifts: 162
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
15	ASN	CG	175.8
41	GLN	CD	179.7
46	GLN	CD	179.5
50	GLN	CD	179.7
51	GLN	CD	178.8
85	ASN	CG	176.8
90	GLN	CD	179.3
106	GLN	CD	180.4
107	ASN	CG	177.0
123	ASN	CG	176.5
136	ASN	CG	177.0
140	GLN	CD	180.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	906	781	86.2
¹³C chemical shifts	684	572	83.6
¹⁵N chemical shifts	170	162	95.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	309	294	95.1
¹³C chemical shifts	300	286	95.3
¹⁵N chemical shifts	149	141	94.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	597	487	81.6
¹³C chemical shifts	384	286	74.5
¹⁵N chemical shifts	21	21	100.0

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Found 1 Document (1.293 seconds)

BMRB: 6071

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Coactosin like protein, : 1 : 36 entities Detail

Interaction partners 1. Coactosin like protein, : 7 interactors Detail

Experiments performed 15 experiments Detail

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Chemical shift validation 3 contents Detail