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A functional SOD-like protein in Bacillus subtilis
Authors
Banci, L., Bertini, I., Cramaro, F., Del Conte, R., Fantoni, A., Quattrone, A., Viezzoli, M.
Assembly
Hypothetical superoxide dismutase-like protein yojM
Entity
1. Hypothetical superoxide dismutase-like protein yojM (polymer, Thiol state: all free), 162 monomers, 17229.86 Da Detail

VVETSAFGHH VQLVNREGKA VGFIEIKESD DEGLDIHISA NSLRPGASLG FHIYEKGSCV RPDFESAGGP FNPLNKEHGF NNPMGHHAGD LPNLEVGADG KVDVIMNAPD TSLKKGSKLN ILDEDGSAFI IHEQADDYLT NPSGNSGARI VCGALLGNNE KQ


Formula weight
17229.86 Da
Source organism
Bacillus subtilis
Exptl. method
NMR
Refine. method
the structure of SOD-like from bacillus subtilis was determined using triple resonance three- and bi-dimensional and homonuclear bi-dimensional NMR technique
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.5 %, Completeness: 83.1 %, Completeness (bb): 95.1 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All83.1 % (1480 of 1781)79.2 % (729 of 920)86.8 % (596 of 687)89.1 % (155 of 174)
Backbone95.1 % (890 of 936)94.0 % (297 of 316)96.1 % (448 of 466)94.2 % (145 of 154)
Sidechain73.7 % (727 of 987)71.5 % (432 of 604)78.5 % (285 of 363)50.0 % (10 of 20)
Aromatic41.5 % (49 of 118)42.4 % (25 of 59)40.7 % (24 of 59)
Methyl95.1 % (154 of 162)97.5 % (79 of 81)92.6 % (75 of 81)

1. Hypothetical superoxide dismutase-like protein

VVETSAFGHH VQLVNREGKA VGFIEIKESD DEGLDIHISA NSLRPGASLG FHIYEKGSCV RPDFESAGGP FNPLNKEHGF NNPMGHHAGD LPNLEVGADG KVDVIMNAPD TSLKKGSKLN ILDEDGSAFI IHEQADDYLT NPSGNSGARI VCGALLGNNE KQ

Sample #1

Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.5 (±0.2)


#NameIsotope labelingTypeConcentration
1Hypothetical superoxide dismutase-like protein[U-15N]1.5 mM
2H2O90 %
3D2O10 %
Sample #2

Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.5 (±0.2)


#NameIsotope labelingTypeConcentration
4Hypothetical superoxide dismutase-like protein[U-15N; U-13C]1.5 mM
5H2O90 %
6D2O10 %

LACS Plot; CA
Referencing offset: 3.03 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 3.03 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.06 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 3.36 ppm, Outliers: 5 Detail
Protein Blocks Logo
Calculated from 30 models in PDB: 1U3N, Strand ID: A Detail


Release date
2005-08-17
Citation
A prokaryotic superoxide dismutase paralog lacking two Cu ligands: from largely unstructured in solution to ordered in the crystal
Banci, L., Bertini, I., Calderone, V., Cramaro, F., Del Conte, R., Fantoni, A., Mangani, S., Quattrone, A., Viezzoli, M.SILVIA.
Proc. Natl. Acad. Sci. U. S. A. (2005), 102, 7541-7546, PubMed 15897454 , DOI 10.1073/pnas.0502450102 ,
Related entities 1. Hypothetical superoxide dismutase-like protein yojM, : 1 : 2 : 1 : 167 entities Detail
Interaction partners 1. Hypothetical superoxide dismutase-like protein yojM, : 20 interactors Detail
Experiments performed 14 experiments Detail
Keywords SOD-like, bacillus subtilis, BsSOD, solution structure, NMR