BMRBj - BMREntryMaster

Found 1 Document (3.538 seconds)

BMRB: 6093

1POZ

SOLUTION STRUCTURE OF THE HYALURONAN BINDING DOMAIN OF HUMAN CD44

Authors

Teriete, P., Banerji, S., Blundell, C.D., Kahmann, J.D., Pickford, A.R., Wright, A., Campbell, I.D., Jackson, D.G., Day, A.J.

Assembly

CD44 antigen

Entity

1. CD44 antigen (polymer, Thiol state: all disulfide bound), 159 monomers, 17586.35 Da Detail

AQIDLNITCR FAGVFHVEKN GRYSISRTEA ADLCKAFNST LPTMAQMEKA LSIGFETCRY GFIEGHVVIP RIHPNSICAA NNTGVYILTS NTSQYDTYCF NASAPPEEDC TSVTDLPNAF DGPITITIVN RDGTRYVQKG EYRTNPEDIY PSNPTDDDV

Formula weight

17586.35 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS9:SG	1:CYS110:SG
2	disulfide	sing	1:CYS34:SG	1:CYS99:SG
3	disulfide	sing	1:CYS58:SG	1:CYS78:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 76.2 %, Completeness (bb): 84.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	76.2 % (1352 of 1774)	79.2 % (722 of 912)	66.7 % (465 of 697)	100.0 % (165 of 165)
Backbone	84.4 % (788 of 934)	98.4 % (312 of 317)	69.9 % (327 of 468)	100.0 % (149 of 149)
Sidechain	71.8 % (711 of 990)	68.9 % (410 of 595)	75.2 % (285 of 379)	100.0 % (16 of 16)
Aromatic	6.8 % (10 of 146)	13.7 % (10 of 73)	0.0 % (0 of 73)
Methyl	95.2 % (160 of 168)	90.5 % (76 of 84)	100.0 % (84 of 84)

1. CD44

AQIDLNITCR FAGVFHVEKN GRYSISRTEA ADLCKAFNST LPTMAQMEKA LSIGFETCRY GFIEGHVVIP RIHPNSICAA NNTGVYILTS NTSQYDTYCF NASAPPEEDC TSVTDLPNAF DGPITITIVN RDGTRYVQKG EYRTNPEDIY PSNPTDDDV

Show sample condition

Sample #1

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Concentration
1	CD44	[U-13C; U-15N]	1 mM
2	D2O		5 %
3	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Concentration
4	CD44	[U-15N]	1 mM
5	D2O		5 %
6	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Type	Concentration
7	CD44	[U-15N]		1 mM
8	D2O			100 %

Sample #4

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Concentration
9	CD44	1 mM
10	D2O	5 %
11	H2O	95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.75 ppm	external	indirect	0.2514495
¹H	water	protons	4.75 ppm	external	indirect	1.0
¹⁵N	water	protons	4.75 ppm	external	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.75 ppm	external	indirect	0.2514495
¹H	water	protons	4.75 ppm	external	indirect	1.0
¹⁵N	water	protons	4.75 ppm	external	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.75 ppm	external	indirect	0.2514495
¹H	water	protons	4.75 ppm	external	indirect	1.0
¹⁵N	water	protons	4.75 ppm	external	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1POZ, Strand ID: A Detail

Release date

2004-02-04

Citation

Structure of the regulatory hyaluronan binding domain in the inflammatory leukocyte homing receptor CD44
Teriete, P., Banerji, S., Noble, M., Blundell, C.D., Wright, A.J., Pickford, A.R., Lowe, E., Mahoney, D.J., Tammi, M.I., Kahmann, J.D., Campbell, I.D., Day, A.J., Jackson, D.G.
Mol. Cell (2004), 13, 483-496, PubMed 14992719 , DOI: , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P16070 released on 1990-04-01
Title CD44_HUMAN Entity CD44 antigen

Related entities 1. CD44 antigen, : 1 : 3 : 3 : 39 entities Detail

Interaction partners 1. CD44 antigen, : 55 interactors Detail

More details for 55 interactors identified by 21 citations

Experiments performed 8 experiments Detail

1 3D_15N-separated_NOESY

Instrument

#1 Model GE Omega (500 MHz)

#2 Model GE Omega (600 MHz)

#3 Model GE Omega (750 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Concentration
1	CD44	[U-13C; U-15N]	1 mM
2	D2O		5 %
3	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Concentration
4	CD44	[U-15N]	1 mM
5	D2O		5 %
6	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Type	Concentration
7	CD44	[U-15N]		1 mM
8	D2O			100 %

Sample #4

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Concentration
9	CD44	1 mM
10	D2O	5 %
11	H2O	95 %

2 2D NOESY

Instrument

#1 Model GE Omega (500 MHz)

#2 Model GE Omega (600 MHz)

#3 Model GE Omega (750 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Concentration
1	CD44	[U-13C; U-15N]	1 mM
2	D2O		5 %
3	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Concentration
4	CD44	[U-15N]	1 mM
5	D2O		5 %
6	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Type	Concentration
7	CD44	[U-15N]		1 mM
8	D2O			100 %

Sample #4

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Concentration
9	CD44	1 mM
10	D2O	5 %
11	H2O	95 %

3 2D TOCSY

Instrument

#1 Model GE Omega (500 MHz)

#2 Model GE Omega (600 MHz)

#3 Model GE Omega (750 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Concentration
1	CD44	[U-13C; U-15N]	1 mM
2	D2O		5 %
3	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Concentration
4	CD44	[U-15N]	1 mM
5	D2O		5 %
6	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Type	Concentration
7	CD44	[U-15N]		1 mM
8	D2O			100 %

Sample #4

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Concentration
9	CD44	1 mM
10	D2O	5 %
11	H2O	95 %

4 HMQC-J

Instrument

#1 Model GE Omega (500 MHz)

#2 Model GE Omega (600 MHz)

#3 Model GE Omega (750 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Concentration
1	CD44	[U-13C; U-15N]	1 mM
2	D2O		5 %
3	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Concentration
4	CD44	[U-15N]	1 mM
5	D2O		5 %
6	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Type	Concentration
7	CD44	[U-15N]		1 mM
8	D2O			100 %

Sample #4

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Concentration
9	CD44	1 mM
10	D2O	5 %
11	H2O	95 %

5 CBCA(CO)NH

Instrument

#1 Model GE Omega (500 MHz)

#2 Model GE Omega (600 MHz)

#3 Model GE Omega (750 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Concentration
1	CD44	[U-13C; U-15N]	1 mM
2	D2O		5 %
3	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Concentration
4	CD44	[U-15N]	1 mM
5	D2O		5 %
6	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Type	Concentration
7	CD44	[U-15N]		1 mM
8	D2O			100 %

Sample #4

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Concentration
9	CD44	1 mM
10	D2O	5 %
11	H2O	95 %

6 HCCH-TOCSY

Instrument

#1 Model GE Omega (500 MHz)

#2 Model GE Omega (600 MHz)

#3 Model GE Omega (750 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Concentration
1	CD44	[U-13C; U-15N]	1 mM
2	D2O		5 %
3	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Concentration
4	CD44	[U-15N]	1 mM
5	D2O		5 %
6	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Type	Concentration
7	CD44	[U-15N]		1 mM
8	D2O			100 %

Sample #4

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Concentration
9	CD44	1 mM
10	D2O	5 %
11	H2O	95 %

7 CC(CO)NH

Instrument

#1 Model GE Omega (500 MHz)

#2 Model GE Omega (600 MHz)

#3 Model GE Omega (750 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Concentration
1	CD44	[U-13C; U-15N]	1 mM
2	D2O		5 %
3	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Concentration
4	CD44	[U-15N]	1 mM
5	D2O		5 %
6	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Type	Concentration
7	CD44	[U-15N]		1 mM
8	D2O			100 %

Sample #4

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Concentration
9	CD44	1 mM
10	D2O	5 %
11	H2O	95 %

8 NH-Exchange HSQC

Instrument

#1 Model GE Omega (500 MHz)

#2 Model GE Omega (600 MHz)

#3 Model GE Omega (750 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Concentration
1	CD44	[U-13C; U-15N]	1 mM
2	D2O		5 %
3	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Concentration
4	CD44	[U-15N]	1 mM
5	D2O		5 %
6	H2O		95 %

Sample #3

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Isotope labeling	Type	Concentration
7	CD44	[U-15N]		1 mM
8	D2O			100 %

Sample #4

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.15)

#	Name	Concentration
9	CD44	1 mM
10	D2O	5 %
11	H2O	95 %

nullKeywords CARBOHYDRATE-BINDING DOMAIN, CELL ADHESION, GLYCOPROTEIN, HYALURONAN-BINDING DOMAIN, LINK MODULE

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Found 1 Document (3.538 seconds)

BMRB: 6093

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. CD44 antigen, : 1 : 3 : 3 : 39 entities Detail

Interaction partners 1. CD44 antigen, : 55 interactors Detail

Experiments performed 8 experiments Detail

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