BMRB: 6097

Proton chemical shift assignments for human spliceosomal protein U1C zinc finger domain

Authors

Muto, Y., Pomeranz Krummel, D., Oubridge, C., Hernandez, H., Robinson, C.V., Neuhaus, D., Nagai, K.

Assembly

U1C zinc finger domain

Entity

1. U1C protein (polymer, Thiol state: not reported), 61 monomers, 7266.079 Da Detail

MPKFYCDYCD TYLTHDSPSV RKTHCSGRKH KENVKDYYQK WMEEQAQSLI DKTTAAFQQG K

2. ZN (non-polymer), 65.409 Da

Total weight

7331.4883 Da

Max. entity weight

7266.079 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.4 %, Completeness: 88.2 %, Completeness (bb): 98.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	88.2 % (344 of 390)	88.2 % (344 of 390)
Backbone	98.4 % (120 of 122)	98.4 % (120 of 122)
Sidechain	83.6 % (224 of 268)	83.6 % (224 of 268)
Aromatic	100.0 % (42 of 42)	100.0 % (42 of 42)
Methyl	94.4 % (17 of 18)	94.4 % (17 of 18)

1. U1C protein

MPKFYCDYCD TYLTHDSPSV RKTHCSGRKH KENVKDYYQK WMEEQAQSLI DKTTAAFQQG K

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	U1C protein			0.8 ~ 1.0 mM
2	potassium phosphate			20 mM
3	magnesium sulphate			100 mM
4	sodium bromide			400 mM

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Protein Blocks Logo

Calculated from 47 models in PDB: 2VRD, Strand ID: A Detail

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Release date

2004-09-27

Citation

The structure and biochemical properties of the human spliceosomal protein U1C
Muto, Y., Pomeranz Krummel, D., Oubridge, C., Hernandez, H., Robinson, C.V., Neuhaus, D., Nagai, K.
J. Mol. Biol. (2004), 341, 185-198, PubMed 15312772 , DOI 10.1016/j.jmb.2004.04.078 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. U1C protein, : 1 : 11 : 1 : 64 entities Detail

Interaction partners 1. U1C protein, : 6 interactors Detail

Experiments performed 3 experiments Detail

Chemical shift validation 4 contents Detail