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BMRB: 6121

2DHS

1H, 15N, and 13C chemical shift assignments of CUG binding protein-CUGBP

Authors

Jun, K., Xia, Y., Han, X., Timchenko, L.T., Swanson, M.S., Gao, X.

Assembly

CUG-BP

Entity

1. CUG-BP (polymer, Thiol state: all free), 187 monomers, 21078.03 Da Detail

MNGTLDHPDQ PDLDAIKMFV GQVPRTWSEK DLRELFEQYG AVYEINVLRD RSQNPPQSKG CCFVTFYTRK AALEAQNALH NMKVLPGMHH PIQMKPADSE KNNAVEDRKL FIGMISKKCT ENDIRVMFSS FGQIEECRIL RGPDGLSRGC AFVTFTTRAM AQTAIKAMHQ AQTMEGCSSP MVVKFAD

Formula weight

21078.03 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.9 %, Completeness (bb): 98.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.9 % (1961 of 2181)	89.8 % (1026 of 1143)	88.8 % (747 of 841)	95.4 % (188 of 197)
Backbone	98.0 % (1080 of 1102)	97.9 % (367 of 375)	98.4 % (541 of 550)	97.2 % (172 of 177)
Sidechain	83.6 % (1049 of 1255)	84.9 % (652 of 768)	81.6 % (381 of 467)	80.0 % (16 of 20)
Aromatic	39.1 % (61 of 156)	59.0 % (46 of 78)	18.2 % (14 of 77)	100.0 % (1 of 1)
Methyl	98.9 % (172 of 174)	98.9 % (86 of 87)	98.9 % (86 of 87)

1. CUG-BP

Show sample condition

Sample

Temperature 298 (±0.05) K, pH 5.8 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	CUG-BP	[U-95% 13C; U-90% 15N]		0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type
¹³C	DSS	methyl protons	0.0 ppm	external	indirect
¹H	water	protons	4.76 ppm	internal	direct
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect

Referencing offset: -0.15 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type
¹³C	DSS	methyl protons	0.0 ppm	external	indirect
¹H	water	protons	4.76 ppm	internal	direct
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect

Referencing offset: -0.15 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type
¹³C	DSS	methyl protons	0.0 ppm	external	indirect
¹H	water	protons	4.76 ppm	internal	direct
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type
¹³C	DSS	methyl protons	0.0 ppm	external	indirect
¹H	water	protons	4.76 ppm	internal	direct
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 7 models in PDB: 2DHS, Strand ID: A Detail

Release date

2004-12-15

Citation

1H, 15N, and 13C chemical shift assignments of RNA repeats binding protein - CUGBP1ab
Jun, K., Xia, Y., Han, X., Zhang, H., Timchenko, L.T., Swanson, M.S., Gao, X.
J. Biomol. NMR (2004), 30, 371-372, PubMed , DOI:

Related entities 1. CUG-BP, : 1 : 2 : 4 : 236 entities Detail

Interaction partners 1. CUG-BP, : 28 interactors Detail

More details for 28 interactors identified by 8 citations

Experiments performed 17 experiments Detail

1 HSQC