BMRB: 6122

1H/13C/15N assignment of the S1 domain of RNase E of E. coli

Authors

Schubert, M., Edge, R.E., Lario, P., Cook, M.A., Strynadka, N.C., Mackie, G.A., McIntosh, L.P.

Assembly

S1 monomer

Entity

1. S1 monomer (polymer, Thiol state: not present), 96 monomers, 10686.99 Da Detail

GSHMLEQKKA NIYKGKITRI EPSLEAAFVD YGAERHGFLP LKEIAREYFP ANYSAHGRPN IKDVLREGQE VIVQIDKEER GNKGAALTTF ISLAGS

Formula weight

10686.99 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.8 %, Completeness: 91.3 %, Completeness (bb): 93.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.3 % (1026 of 1124)	91.7 % (540 of 589)	89.9 % (392 of 436)	94.9 % (94 of 99)
Backbone	93.7 % (532 of 568)	94.4 % (186 of 197)	92.8 % (259 of 279)	94.6 % (87 of 92)
Sidechain	89.9 % (578 of 643)	90.3 % (354 of 392)	88.9 % (217 of 244)	100.0 % (7 of 7)
Aromatic	53.6 % (45 of 84)	57.1 % (24 of 42)	50.0 % (21 of 42)
Methyl	100.0 % (102 of 102)	100.0 % (51 of 51)	100.0 % (51 of 51)

1. S1 Rnase E

GSHMLEQKKA NIYKGKITRI EPSLEAAFVD YGAERHGFLP LKEIAREYFP ANYSAHGRPN IKDVLREGQE VIVQIDKEER GNKGAALTTF ISLAGS

Show sample condition

Sample

Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	S1 Rnase E	[U-13C; U-15N]		1.5 mM

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LACS Plot; CA

Referencing offset: -0.42 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	direct	0.1013291
13C	DSS	methyl protons	0.0 ppm	external	direct	0.2514495

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LACS Plot; CB

Referencing offset: -0.42 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	direct	0.1013291
13C	DSS	methyl protons	0.0 ppm	external	direct	0.2514495

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 5 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	direct	0.1013291
13C	DSS	methyl protons	0.0 ppm	external	direct	0.2514495

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LACS Plot; CO

Referencing offset: 0.41 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	direct	0.1013291
13C	DSS	methyl protons	0.0 ppm	external	direct	0.2514495

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Protein Blocks Logo

Calculated from 10 models in PDB: 1SLJ, Strand ID: A Detail

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Release date

2004-08-31

Citation

Structural characterization of the RNase E S1 domain and identification of its oligonucleotide-binding and dimerization interfaces
Schubert, M., Edge, R.E., Lario, P., Cook, M.A., Strynadka, N.C., Mackie, G.A., McIntosh, L.P.
J. Mol. Biol. (2004), 341, 37-54, PubMed 15312761 , DOI 10.1016/j.jmb.2004.05.061 , Abstract

Related entities 1. S1 monomer, : 1 : 2 : 11 : 26 entities Detail

Interaction partners 1. S1 monomer, : 30 interactors Detail

Experiments performed 9 experiments Detail