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Found 1 Document (1.987 seconds)

BMRB: 6130

1S6O

Solution structure and backbone dynamics of the Cu(I) and apo-forms of the second metal-binding domain of the Menkes protein ATP7A

Authors

Banci, L., Bertini, I., Del Conte, R., D'Onofrio, M., Rosato, A.

Assembly

Copper-transporting ATPase 1 (E.C.3.6.3.4)

Entity

1. Copper-transporting ATPase 1 (E.C.3.6.3.4) (polymer, Thiol state: not reported), 76 monomers, 8446.875 Da Detail

GEVVLKMKVE GMTCHSCTST IEGKIGKLQG VQRIKVSLDN QEATIVYQPH LISVEEMKKQ IEAMGFPAFV KKIEGR

Formula weight

8446.875 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

restrained energy minimization

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.1 %, Completeness (bb): 98.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.1 % (848 of 901)	94.1 % (445 of 473)	93.1 % (324 of 348)	98.8 % (79 of 80)
Backbone	98.5 % (445 of 452)	98.1 % (154 of 157)	98.6 % (218 of 221)	98.6 % (73 of 74)
Sidechain	91.1 % (472 of 518)	92.1 % (291 of 316)	89.3 % (175 of 196)	100.0 % (6 of 6)
Aromatic	50.0 % (18 of 36)	88.9 % (16 of 18)	11.1 % (2 of 18)
Methyl	100.0 % (90 of 90)	100.0 % (45 of 45)	100.0 % (45 of 45)

1. ATPase

GEVVLKMKVE GMTCHSCTST IEGKIGKLQG VQRIKVSLDN QEATIVYQPH LISVEEMKKQ IEAMGFPAFV KKIEGR

Show sample condition

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
1	ATPase	1 mM
2	phosphate	100 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
5	ATPase	[U-15N]	1 mM
6	phosphate		100 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
9	ATPase	[U-13C; U-15N]	1 mM
10	phosphate		100 mM
11	H2O		90 %
12	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 2.09 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 2.09 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 2.76 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 30 models in PDB: 1S6O, Strand ID: A Detail

Release date

2004-05-14

Citation

Solution structure and backbone dynamics of the Cu(I) and apo forms of the second metal-binding domain of the Menkes protein ATP7A
Banci, L., Bertini, I., Del Conte, R., D'Onofrio, M., Rosato, A.
Biochemistry (2004), 43, 3396-3403, PubMed 15035611 , DOI 10.1021/bi036042s , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 6129 released on 2004-05-14
Title Solution structure and backbone dynamics of the Cu(I) and apo-forms of the second metal-binding domain of the Menkes protein ATP7A

Related entities 1. Copper-transporting ATPase 1 (E.C.3.6.3.4), : 1 : 1 : 3 : 282 entities Detail

Interaction partners 1. Copper-transporting ATPase 1 (E.C.3.6.3.4), : 5 interactors Detail

More details for 5 interactors identified by 3 citations

Experiments performed 4 experiments Detail

1 [1H-1H]-NOESY

Instrument

Model na unknown (na MHz) Details spectrometer information not available

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
1	ATPase	1 mM
2	phosphate	100 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
5	ATPase	[U-15N]	1 mM
6	phosphate		100 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
9	ATPase	[U-13C; U-15N]	1 mM
10	phosphate		100 mM
11	H2O		90 %
12	D2O		10 %

2 13C-NOESY-HSQC

Instrument

Model na unknown (na MHz) Details spectrometer information not available

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
1	ATPase	1 mM
2	phosphate	100 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
5	ATPase	[U-15N]	1 mM
6	phosphate		100 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
9	ATPase	[U-13C; U-15N]	1 mM
10	phosphate		100 mM
11	H2O		90 %
12	D2O		10 %

3 15N-NOESY-HSQC

Instrument

Model na unknown (na MHz) Details spectrometer information not available

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
1	ATPase	1 mM
2	phosphate	100 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
5	ATPase	[U-15N]	1 mM
6	phosphate		100 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
9	ATPase	[U-13C; U-15N]	1 mM
10	phosphate		100 mM
11	H2O		90 %
12	D2O		10 %

4 HNHA

Instrument

Model na unknown (na MHz) Details spectrometer information not available

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
1	ATPase	1 mM
2	phosphate	100 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
5	ATPase	[U-15N]	1 mM
6	phosphate		100 mM
7	H2O		90 %
8	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
9	ATPase	[U-13C; U-15N]	1 mM
10	phosphate		100 mM
11	H2O		90 %
12	D2O		10 %

nullKeywords copper homeostasis, Menkes, metal transport

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Found 1 Document (1.987 seconds)

BMRB: 6130

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Copper-transporting ATPase 1 (E.C.3.6.3.4), : 1 : 1 : 3 : 282 entities Detail

Interaction partners 1. Copper-transporting ATPase 1 (E.C.3.6.3.4), : 5 interactors Detail

Experiments performed 4 experiments Detail

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