BMRB: 6138

Hypothetical protein At2g24940.1 from Arabidopsis thaliana has a cytochrome b5 like fold

Authors

Song, J., Vinarov, D.A., Tyler, E.M., Shahan, M.N., Tyler, R.C., Markley, J.L.

Assembly

At2g24940

Entity

1. At2g24940 (polymer, Thiol state: not present), 109 monomers, 12153.28 Da Detail

MGHHHHHHLE EFTAEQLSQY NGTDESKPIY VAIKGRVFDV TTGKSFYGSG GDYSMFAGKD ASRALGKMSK NEEDVSPSLE GLTEKEINTL NDWETKFEAK YPVVGRVVS

Formula weight

12153.28 Da

Source organism

Arabidopsis thaliana

Exptl. method

NMR

Refine. method

molecular dynamics, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.5 %, Completeness: 83.6 %, Completeness (bb): 92.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	83.6 % (1038 of 1241)	83.2 % (534 of 642)	81.7 % (397 of 486)	94.7 % (107 of 113)
Backbone	92.9 % (602 of 648)	93.8 % (212 of 226)	91.8 % (290 of 316)	94.3 % (100 of 106)
Sidechain	76.3 % (527 of 691)	77.4 % (322 of 416)	73.9 % (198 of 268)	100.0 % (7 of 7)
Aromatic	23.0 % (29 of 126)	38.1 % (24 of 63)	6.5 % (4 of 62)	100.0 % (1 of 1)
Methyl	100.0 % (94 of 94)	100.0 % (47 of 47)	100.0 % (47 of 47)

1. At2g24940

MGHHHHHHLE EFTAEQLSQY NGTDESKPIY VAIKGRVFDV TTGKSFYGSG GDYSMFAGKD ASRALGKMSK NEEDVSPSLE GLTEKEINTL NDWETKFEAK YPVVGRVVS

Show sample condition

Sample

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	At2g24940			0.5 mM
2	KH2PO4			10 mM
3	KCl			50 mM
4	NaN3			0.02 mM
5	DSS			1 mM
6	H2O			90 %
7	D2O			10 %

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LACS Plot; CA

Referencing offset: 0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	direct	0.1013291
13C	DSS	methyl protons	0.0 ppm	external	direct	0.2514495

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LACS Plot; CB

Referencing offset: 0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	direct	0.1013291
13C	DSS	methyl protons	0.0 ppm	external	direct	0.2514495

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	direct	0.1013291
13C	DSS	methyl protons	0.0 ppm	external	direct	0.2514495

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LACS Plot; CO

Referencing offset: -0.32 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	direct	0.1013291
13C	DSS	methyl protons	0.0 ppm	external	direct	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 1T0G, Strand ID: A Detail

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Release date

2004-07-25

Citation

Hypothetical protein At2g24940.1 from Arabidopsis thaliana has a cytochrome b5 like fold
Song, J., Vinarov, D.A., Tyler, E.M., Shahan, M.N., Tyler, R.C., Markley, J.L.
J. Biomol. NMR (2004), 30, 215-218

Related entities 1. At2g24940, : 1 : 3 : 93 entities Detail

Interaction partners 1. At2g24940, : 32 interactors Detail

Experiments performed 1 experiments Detail