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Solution Structure of Ole e 6, an allergen from olive tree pollen
Authors
Trevino, M.A., Garcia-Mayoral, M., Barral, P., Villalba, M., Rico, M., Santoro, J., Rodriguez, R., Bruix, M.
Assembly
Oela europea pollen allergen ole e 6
Entity
1. Oela europea pollen allergen ole e 6 (polymer, Thiol state: all disulfide bound), 50 monomers, 5833.448 Da Detail

DEAQFKECYD TCHKECSDKG NGFTFCEMKC DTDCSVKDVK EKLENYKPKN


Formula weight
5833.448 Da
Entity Connection
disulfide 3 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing1:CYS8:SG1:CYS34:SG
2disulfidesing1:CYS12:SG1:CYS30:SG
3disulfidesing1:CYS16:SG1:CYS26:SG

Source organism
Olea europaea
Exptl. method
NMR
Refine. method
simulated annealing, torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 100.0 %, Completeness: 99.4 %, Completeness (bb): 98.7 % Detail

Polymer type: polypeptide(L)

Total1H15N
All99.4 % (360 of 362)99.7 % (308 of 309)98.1 % (52 of 53)
Backbone98.7 % (148 of 150)99.0 % (100 of 101)98.0 % (48 of 49)
Sidechain100.0 % (212 of 212)100.0 % (208 of 208)100.0 % (4 of 4)
Aromatic100.0 % (25 of 25)100.0 % (25 of 25)
Methyl100.0 % (10 of 10)100.0 % (10 of 10)

1. Olea europea pollen allergen ole e 6

DEAQFKECYD TCHKECSDKG NGFTFCEMKC DTDCSVKDVK EKLENYKPKN

Sample #1

Temperature 308 (±0.1) K, pH 6.0 (±0.1)


#NameIsotope labelingTypeConcentration
1Olea europea pollen allergen ole e 60.6 ~ 1.0 mM
2H2O95 %
3D2O5 %
Sample #2

Temperature 308 (±0.1) K, pH 6.0 (±0.1)


#NameIsotope labelingTypeConcentration
4Olea europea pollen allergen ole e 60.6 ~ 1.0 mM
5D2O100 %
Sample #3

Temperature 308 (±0.1) K, pH 6.0 (±0.1)


#NameIsotope labelingTypeConcentration
6Olea europea pollen allergen ole e 6[U-15N]0.6 ~ 1.0 mM
7H2O95 %
8D2O5 %

Chem. Shift Complete2
Sequence coverage: 96.0 %, Completeness: 82.9 %, Completeness (bb): 66.3 % Detail

Polymer type: polypeptide(L)

Total1H15N
All82.9 % (600 of 724)88.3 % (546 of 618)50.9 % (54 of 106)
Backbone66.3 % (199 of 300)73.8 % (149 of 202)51.0 % (50 of 98)
Sidechain94.6 % (401 of 424)95.4 % (397 of 416)50.0 % (4 of 8)
Aromatic100.0 % (50 of 50)100.0 % (50 of 50)
Methyl100.0 % (20 of 20)100.0 % (20 of 20)

1. Olea europea pollen allergen ole e 6

DEAQFKECYD TCHKECSDKG NGFTFCEMKC DTDCSVKDVK EKLENYKPKN

Sample #1

Temperature 308 (±0.1) K, pH 6.0 (±0.1)


#NameIsotope labelingTypeConcentration
1Olea europea pollen allergen ole e 60.6 ~ 1.0 mM
2H2O95 %
3D2O5 %
Sample #2

Temperature 308 (±0.1) K, pH 6.0 (±0.1)


#NameIsotope labelingTypeConcentration
4Olea europea pollen allergen ole e 60.6 ~ 1.0 mM
5D2O100 %
Sample #3

Temperature 308 (±0.1) K, pH 6.0 (±0.1)


#NameIsotope labelingTypeConcentration
6Olea europea pollen allergen ole e 6[U-15N]0.6 ~ 1.0 mM
7H2O95 %
8D2O5 %

Protein Blocks Logo
Calculated from 25 models in PDB: 1SS3, Strand ID: A Detail


Release date
2004-03-10
Citation
NMR solution structure of Ole e 6, a major allergen from olive tree pollen
Trevino, M.A., Garcia-Mayoral, M., Barral, P., Villalba, M., Santoro, J., Rico, M., Rodriguez, R., Bruix, M.
J. Biol. Chem. (2004), 279, 39035-39041, PubMed 15247256 , DOI 10.1074/jbc.M406045200 ,
Entries sharing articles Swiss-Prot: 1 entries Detail
  Swiss-Prot: O24172 released on 2001-06-20
    Title ALL6_OLEEU Entity Major pollen allergen Ole e 6
Related entities 1. Oela europea pollen allergen ole e 6, : 1 : 1 : 3 entities Detail
Experiments performed 5 experiments Detail
nullKeywords allergy pollinosis olive