BMRBj - BMREntryMaster

Found 1 Document (1.511 seconds)

BMRB: 6181

1VET

Resonance Assignments for the Endosomal Adaptor Protein P14

Authors

Qian, C., Zeng, L., Farooq, A., Zhou, M.

Assembly

endosomal adaptor protein P14

Entity

1. endosomal adaptor protein P14 (polymer, Thiol state: not reported), 125 monomers, 13480.34 Da Detail

MLRPKALTQV LSQANTGGVQ STLLLNNEGS LLAYSGYGDT DARVTAAIAS NIWAAYDRNG NQAFNEDSLK FILMDCMEGR VAITRVANLL LCMYAKETVG FGMLKAKAQA LVQYLEEPLT QVAAS

Formula weight

13480.34 Da

Source organism

Mus musculus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 75.0 %, Completeness (bb): 83.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	75.0 % (1051 of 1401)	77.1 % (553 of 717)	69.4 % (378 of 545)	86.3 % (120 of 139)
Backbone	83.4 % (622 of 746)	95.3 % (245 of 257)	70.8 % (259 of 366)	95.9 % (118 of 123)
Sidechain	70.6 % (544 of 771)	67.0 % (308 of 460)	79.3 % (234 of 295)	12.5 % (2 of 16)
Aromatic	53.7 % (44 of 82)	53.7 % (22 of 41)	55.0 % (22 of 40)	0.0 % (0 of 1)
Methyl	86.3 % (145 of 168)	88.1 % (74 of 84)	84.5 % (71 of 84)

1. P14

MLRPKALTQV LSQANTGGVQ STLLLNNEGS LLAYSGYGDT DARVTAAIAS NIWAAYDRNG NQAFNEDSLK FILMDCMEGR VAITRVANLL LCMYAKETVG FGMLKAKAQA LVQYLEEPLT QVAAS

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Concentration
1	P14	0.0 ~ 0.0 mM
2	NaPi	50 mM
3	NaCl	250 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
¹H	H2O	protons	4.79 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
¹H	H2O	protons	4.79 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
¹H	H2O	protons	4.79 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 2 Detail

Release date

2004-12-15

Citation

Resonance assignments for the endosomal adaptor protein p14
Qian, C., Zeng, L., Farooq, A., Zhou, M.
J. Biomol. NMR (2004), 30, 367-368, PubMed 15756467 , DOI 10.1007/s10858-005-1551-4 , Abstract

Related entities 1. endosomal adaptor protein P14, : 1 : 4 : 25 : 4 : 20 entities Detail

Interaction partners 1. endosomal adaptor protein P14, : 4 interactors Detail

More details for 4 interactors identified by 4 citations

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr6181_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr6181_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chemical_shift_1	Warning	1132		98.4 (chain: 1, length: 125)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: chemical_shift_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 125, Sequence coverage: 98.4%
- Total number of assignments
  - ¹H chemical shifts: 669
  - ¹³C chemical shifts: 347
  - ¹⁵N chemical shifts: 116
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	460	274	59.6
¹³C chemical shifts	295	225	76.3
¹⁵N chemical shifts	21	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	89	77	86.5
¹³C chemical shifts	89	75	84.3

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Found 1 Document (1.511 seconds)

BMRB: 6181

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. endosomal adaptor protein P14, : 1 : 4 : 25 : 4 : 20 entities Detail

Interaction partners 1. endosomal adaptor protein P14, : 4 interactors Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 3 contents Detail

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	717	515	71.8
¹³C chemical shifts	545	347	63.7
¹⁵N chemical shifts	144	116	80.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	257	241	93.8
¹³C chemical shifts	250	122	48.8
¹⁵N chemical shifts	123	116	94.3