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NMR Sequential Assignment of Escherichia coli Thioredoxin Utilizing Random Fractional Deuteriation
Authors
LeMaster, D.M., Richards, F.M.
Assembly
thioredoxin
Entity
1. thioredoxin (polymer), 108 monomers, 11690.30 Da Detail

SDKIIHLTDD SFDTDVLKAD GAILVDFWAE WCGPCKMIAP ILDEIADEYQ GKLTVAKLNI DQNPGTAPKY GIRGIPTLLL FKNGEVAATK VGAKSKGQLK EFLDANLA


Formula weight
11690.3 Da
Source organism
Escherichia coli
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 90.7 %, Completeness: 57.3 %, Completeness (bb): 92.4 % Detail

Polymer type: polypeptide(L)

Total1H
All57.3 % (367 of 640)57.3 % (367 of 640)
Backbone92.4 % (195 of 211)92.4 % (195 of 211)
Sidechain40.1 % (172 of 429)40.1 % (172 of 429)
Aromatic 9.5 % (4 of 42) 9.5 % (4 of 42)
Methyl27.1 % (19 of 70)27.1 % (19 of 70)

1. thioredoxin

SDKIIHLTDD SFDTDVLKAD GAILVDFWAE WCGPCKMIAP ILDEIADEYQ GKLTVAKLNI DQNPGTAPKY GIRGIPTLLL FKNGEVAATK VGAKSKGQLK EFLDANLA

Sample

Temperature 303 K, pH 5.7



Release date
1995-07-30
Citation
NMR sequential assignment of Escherichia coli thioredoxin utilizing random fractional deuteriation
LeMaster, D.M., Richards, F.M.
Biochemistry (1988), 27, 142-150, PubMed 3280013 , DOI 10.1021/bi00401a022 ,
Related entities 1. thioredoxin, : 1 : 55 : 142 entities Detail
Interaction partners 1. thioredoxin, : 23 interactors Detail