BMRB: 6201

Sequence specific 1H and 15N resonance assignment of domain 1 of rat CD2 with the designed calcium binding site

Authors

Yang, W., Yang, J.J.

Assembly

Ca.CD2

Entity

1. Ca.CD2 (polymer, Thiol state: not present), 99 monomers, 11144.50 Da Detail

RDSGTVWGAL GHGIDLDIPN FQMTDDIDEV RWERGSTLVA EFKRKMKPFL KSGAFEILAN GDLKIKNLTR DDSGTYNVTV YSTNGTRILN KALDLRILE

Formula weight

11144.5 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

ANNEAL

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 97.0 %, Completeness: 90.9 %, Completeness (bb): 95.7 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	15N
All	90.9 % (640 of 704)	90.0 % (538 of 598)	96.2 % (102 of 106)
Backbone	95.7 % (289 of 302)	95.6 % (196 of 205)	95.9 % (93 of 97)
Sidechain	87.3 % (351 of 402)	87.0 % (342 of 393)	100.0 % (9 of 9)
Aromatic	93.2 % (41 of 44)	92.9 % (39 of 42)	100.0 % (2 of 2)
Methyl	100.0 % (59 of 59)	100.0 % (59 of 59)

1. Ca.CD2

RDSGTVWGAL GHGIDLDIPN FQMTDDIDEV RWERGSTLVA EFKRKMKPFL KSGAFEILAN GDLKIKNLTR DDSGTYNVTV YSTNGTRILN KALDLRILE

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Ca.CD2	[U-95% 15N]		1.0 mM
2	KCl			130 mM
3	CaCl2			10 mM
4	PIPES			20 mM

Hide sample codition

Chem. Shift Complete2

Sequence coverage: 97.0 %, Completeness: 82.0 %, Completeness (bb): 95.2 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	15N
All	82.0 % (1155 of 1408)	79.5 % (951 of 1196)	96.2 % (204 of 212)
Backbone	95.2 % (575 of 604)	94.9 % (389 of 410)	95.9 % (186 of 194)
Sidechain	72.1 % (580 of 804)	71.5 % (562 of 786)	100.0 % (18 of 18)
Aromatic	90.9 % (80 of 88)	90.5 % (76 of 84)	100.0 % (4 of 4)
Methyl	74.6 % (88 of 118)	74.6 % (88 of 118)

1. Ca.CD2

RDSGTVWGAL GHGIDLDIPN FQMTDDIDEV RWERGSTLVA EFKRKMKPFL KSGAFEILAN GDLKIKNLTR DDSGTYNVTV YSTNGTRILN KALDLRILE

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Type	Concentration
5	Ca.CD2	[U-95% 15N]		1.0 mM
6	KCl			130 mM
7	EGTA			3 mM
8	PIPES			20 mM

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 1T6W, Strand ID: A Detail

---

Release date

2005-02-15

Citation

Design of a calcium-binding protein with desired structure in a cell adhesion molecule
Yang, W., Wilkins, A.L., Ye, Y., Liu, Z.R., Li, S.Y., Urbauer, J.L., Hellinga, H., Kearney, A., van der Merwe, P.A., Yang, J.J.
J. Am. Chem. Soc. (2005), 127, 2085-2093, PubMed 15713084 , DOI 10.1021/ja0431307 , Abstract

Related entities 1. Ca.CD2, : 1 : 4 : 28 entities Detail

Interaction partners 1. Ca.CD2, : 1 interactors Detail

Experiments performed 1 experiments Detail