BMRB: 6233

(poly)ubiquitin-binding region of S5a

Authors

Wang, Q., Walters, K.J.

Assembly

(poly)ubiquitin-binding region of the proteasome subunit S5a

Entity

1. (poly)ubiquitin-binding region of the proteasome subunit S5a (polymer, Thiol state: not present), 111 monomers, 11819.82 Da Detail

MLGLGASDFE FGVDPSADPE LALALRVSME EQRQRQEEEA RRAAAASAAE AGIATTGTED SDDALLKMTI SQQEFGRTGL PDLSSMTEEE QIAYAMQMSL QGAEFGQAES A

Formula weight

11819.82 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.2 %, Completeness (bb): 97.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.2 % (1042 of 1181)	84.9 % (518 of 610)	89.9 % (408 of 454)	99.1 % (116 of 117)
Backbone	97.3 % (642 of 660)	95.2 % (217 of 228)	98.1 % (318 of 324)	99.1 % (107 of 108)
Sidechain	80.3 % (500 of 623)	78.8 % (301 of 382)	81.9 % (190 of 232)	100.0 % (9 of 9)
Aromatic	20.8 % (10 of 48)	41.7 % (10 of 24)	0.0 % (0 of 24)
Methyl	92.7 % (102 of 110)	94.5 % (52 of 55)	90.9 % (50 of 55)

1. (poly)ubiquitin-binding region of S5a

MLGLGASDFE FGVDPSADPE LALALRVSME EQRQRQEEEA RRAAAASAAE AGIATTGTED SDDALLKMTI SQQEFGRTGL PDLSSMTEEE QIAYAMQMSL QGAEFGQAES A

Show sample condition

Sample

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	(poly)ubiquitin-binding region of S5a	[U-13C; U-15N]		0.35 mM

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LACS Plot; CA

Referencing offset: -0.19 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	external	direct	1.0
13C	TSP	methyl protons	0.0 ppm	external	indirect	0.25146
15N	TSP	methyl protons	0.0 ppm	external	indirect	0.10134

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LACS Plot; CB

Referencing offset: -0.19 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	external	direct	1.0
13C	TSP	methyl protons	0.0 ppm	external	indirect	0.25146
15N	TSP	methyl protons	0.0 ppm	external	indirect	0.10134

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	external	direct	1.0
13C	TSP	methyl protons	0.0 ppm	external	indirect	0.25146
15N	TSP	methyl protons	0.0 ppm	external	indirect	0.10134

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LACS Plot; CO

Referencing offset: 0.55 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	external	direct	1.0
13C	TSP	methyl protons	0.0 ppm	external	indirect	0.25146
15N	TSP	methyl protons	0.0 ppm	external	indirect	0.10134

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Protein Blocks Logo

Calculated from 18 models in PDB: 1YX6, Strand ID: A Detail

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Release date

2004-11-28

Citation

Chemical shift assignments of the (poly)ubiquitin-binding region of the proteasome subunit S5a
Wang, Q., Walters, K.J.
J. Biomol. NMR (2004), 30, 231-232, PubMed 15704274 , DOI: , Abstract

Related entities 1. (poly)ubiquitin-binding region of the proteasome subunit S5a, : 1 : 24 : 5 : 19 entities Detail

Interaction partners 1. (poly)ubiquitin-binding region of the proteasome subunit S5a, : 91 interactors Detail

Experiments performed 5 experiments Detail