Search

Backbone 1H, 13C, and 15N Chemical Shift Assignments for CAPPD*, an Independently Folded Extracellular Domain of Human Amyloid-beta Precursor Protein
Authors
Dulubova, I., Ho, A., Huryeva, I., Sudhof, T.C., Rizo, J.
Assembly
APP770
Entity
1. APP770 (polymer, Thiol state: not present), 117 monomers, 13952.85 Da Detail

RVEAMLNDRR RLALENYITA LQAVPPRPRH VFNMLKKYVR AEQKDRQHTL KHFEHVRMVD PKKAAQIRSQ VMTHLRVIYE RMNQSLSLLY NVPAVAEEIQ DEVDELLQKE QNYSDDV


Formula weight
13952.85 Da
Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 98.4 %, Completeness (bb): 99.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All98.4 % (1425 of 1448)98.8 % (750 of 759)99.3 % (546 of 550)92.8 % (129 of 139)
Backbone99.4 % (688 of 692)100.0 % (229 of 229)98.9 % (347 of 351)100.0 % (112 of 112)
Sidechain97.8 % (854 of 873)98.3 % (521 of 530)100.0 % (316 of 316)63.0 % (17 of 27)
Aromatic100.0 % (80 of 80)100.0 % (40 of 40)100.0 % (40 of 40)
Methyl100.0 % (136 of 136)100.0 % (68 of 68)100.0 % (68 of 68)

1. Amyloid-beta Precursor Protein

RVEAMLNDRR RLALENYITA LQAVPPRPRH VFNMLKKYVR AEQKDRQHTL KHFEHVRMVD PKKAAQIRSQ VMTHLRVIYE RMNQSLSLLY NVPAVAEEIQ DEVDELLQKE QNYSDDV

Sample

Temperature 300 (±0.5) K, pH 6.4 (±0.1)


#NameIsotope labelingTypeConcentration
1Amyloid-beta Precursor Protein[U-99% 15N]0.85 mM
2sodium phosphate50 mM
3NaCl250 mM
4Guanidinium Chloride300 mM
5D2O5 %

LACS Plot; CA
Referencing offset: -0.11 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.11 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.03 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: -0.85 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 1TKN, Strand ID: A Detail


Release date
2004-08-31
Citation
Three-dimensional structure of an independently folded extracellular domain of human amyloid-beta precursor protein
Dulubova, I., Ho, A., Huryeva, I., Sudhof, T.C., Rizo, J.
Biochemistry (2004), 43, 9583-9588, PubMed 15274612 , DOI 10.1021/bi049041o ,
Related entities 1. APP770, : 1 : 7 : 3 : 5 : 15 entities Detail
Interaction partners 1. APP770, : 2 interactors Detail
Experiments performed 5 experiments Detail
Keywords Amyloid-beta Precursor Protein, Alzheimer's disease, CAPPD, CAPPD*, four alpha-helices, three-helix bundle