BMRBj - BMREntryMaster

Found 1 Document (1.391 seconds)

BMRB: 6237

2GCZ

Solution Structure of alpha-Conotoxin OmIA

Authors

Chi, S., Kim, D., Olivera, B.M., McIntosh, J.M., Han, K.

Assembly

alpha-conotoxin OmIA

Entity

1. alpha-conotoxin OmIA (polymer, Thiol state: all disulfide bound), 18 monomers, 1723.956 Da Detail

GCCSHPACNV NNPHICGX

Formula weight

1723.956 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS2:SG	1:CYS8:SG
2	disulfide	sing	1:CYS3:SG	1:CYS16:SG

Source organism

Conus omaria

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 94.4 %, Completeness: 90.6 %, Completeness (bb): 88.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	90.6 % (77 of 85)	90.6 % (77 of 85)
Backbone	88.2 % (30 of 34)	88.2 % (30 of 34)
Sidechain	92.2 % (47 of 51)	92.2 % (47 of 51)
Aromatic	50.0 % (2 of 4)	50.0 % (2 of 4)
Methyl	100.0 % (5 of 5)	100.0 % (5 of 5)

1. alpha-conotoxin OmIA

GCCSHPACNV NNPHICGX

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 4.1

#	Name	Concentration
1	alpha-conotoxin OmIA	4.8 mM
2	H2O	90 %
3	D2O	10 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2GCZ, Strand ID: A Detail

Coupling constant

11 J values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 298 K, pH 4.1 Detail

Release date

2007-08-23

Citation

Solution conformation of a neuronal nicotinic acetylcholine receptor antagonist alpha-conotoxin OmIA that discriminates alpha3 vs. alpha6 nAChR subtypes
Chi, S., Kim, D., Olivera, B.M., McIntosh, J.M., Han, K.
Biochem. Biophys. Res. Commun. (2006), 345, 248-254, PubMed 16678128 , DOI 10.1016/j.bbrc.2006.04.099 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P0C1R7 released on 2006-07-25
Title CA1A_CONOM Entity Alpha-conotoxin OmIA

Related entities 1. alpha-conotoxin OmIA, : 1 : 3 : 68 entities Detail

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:2:CYS:SG	1:8:CYS:SG	unknown	unknown	n/a
1:3:CYS:SG	1:16:CYS:SG	unknown	unknown	n/a

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chemical_shift_set_1	Warning	89		94.4 (chain: 1, length: 18)
1	Chemical shift references	chemical_shift_reference	OK	1		No information

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	87	79	90.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	34	31	91.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	53	48	90.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	5	5	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	4	2	50.0

Keywords alpha-helix, beta-turn, C-terminal amidation, two disulfide bonds

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr6237_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	True (see coordinates for details)
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

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Found 1 Document (1.391 seconds)

BMRB: 6237

Sample

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. alpha-conotoxin OmIA, : 1 : 3 : 68 entities Detail

Experiments performed 3 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail