Search

Query history

Search help 
Download

Found 1 Document (1.818 seconds)

BMRB: 6241


6241
1YZB
Assignment of the 1H, 13C, and 15N resonances of the josephin domain of ataxin-3
Authors
Nicastro, G., Masino, L., Frenkiel, T., Kelly, G., McCormick, J., Menon, R., Pastore, A.
Assembly
josephin domain of ataxin-3
Entity
1. josephin domain of ataxin-3 (polymer, Thiol state: all free), 182 monomers, 21035.59 Da Detail

MESIFHEKQE GSLCAQHCLN NLLQGEYFSP VELSSIAHQL DEEERMRMAE GGVTSEDYRT FLQQPSGNMD DSGFFSIQVI SNALKVWGLE LILFNSPEYQ RLRIDPINER SFICNYKEHW FTVRKLGKQW FNLNSLLTGP ELISDTYLAL FLAQLQQEGY SIFVVKGDLP DCEADQLLQM IR


Formula weight
21035.59 Da
Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
simulated annealing, torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 97.3 %, Completeness (bb): 97.9 % Detail
Download

Polymer type: polypeptide(L)

Total1H13C15N
All97.3 % (2135 of 2194)98.3 % (1124 of 1144)95.8 % (812 of 848)98.5 % (199 of 202)
Backbone97.9 % (1057 of 1080)99.2 % (366 of 369)96.6 % (517 of 535)98.9 % (174 of 176)
Sidechain97.1 % (1248 of 1285)97.8 % (758 of 775)96.1 % (465 of 484)96.2 % (25 of 26)
Aromatic91.0 % (191 of 210)92.4 % (97 of 105)89.2 % (91 of 102)100.0 % (3 of 3)
Methyl97.9 % (188 of 192)99.0 % (95 of 96)96.9 % (93 of 96)

1. ataxin-3

MESIFHEKQE GSLCAQHCLN NLLQGEYFSP VELSSIAHQL DEEERMRMAE GGVTSEDYRT FLQQPSGNMD DSGFFSIQVI SNALKVWGLE LILFNSPEYQ RLRIDPINER SFICNYKEHW FTVRKLGKQW FNLNSLLTGP ELISDTYLAL FLAQLQQEGY SIFVVKGDLP DCEADQLLQM IR

Show sample condition
Sample

Temperature 298 (±0.5) K, pH 6.5 (±0.2)


#NameIsotope labelingTypeConcentration
1ataxin-3[U-95% 13C; U-95% 15N]0.4 mM

LACS Plot; CA
Referencing offset: -0.16 ppm, Outliers: 5 Detail
LACS Plot; CB
Referencing offset: -0.16 ppm, Outliers: 5 Detail
LACS Plot; HA
Referencing offset: 0.0 ppm, Outliers: 4 Detail
LACS Plot; CO
Referencing offset: 0.02 ppm, Outliers: 8 Detail
Protein Blocks Logo
Calculated from 10 models in PDB: 1YZB, Strand ID: A Detail

Release date
2005-01-06
Citation 1
Assignment of the 1H, 13C, and 15N resonances of the Josephin domain of human ataxin-3
Nicastro, G., Masino, L., Frenkiel, T.A., Kelly, G., McCormick, J., Menon, R.P., Pastore, A.
J. Biomol. NMR (2004), 30, 457-458, PubMed 15630566 , DOI 10.1007/s10858-004-4343-3 ,
Show more 2 citaions
Citation 2
Domain architecture of the polyglutamine protein ataxin-3: a globular domain followed by a flexible tail
Masino, L., Musi, V., Menon, R.P., Fusi, P., Kelly, G., Frenkiel, T.A., Trottier, Y., Pastore, A.
FEBS Lett. (2003), 549, 21-25, PubMed 12914917 , DOI: ,
Citation 3
Glutamine repeats: structural hypotheses and neurodegeneration
Masino, L., Pastore, A.
Biochem. Soc. Trans. (2002), 30, 548-551, PubMed 12196134 , DOI 10.1042/ ,
Related entities 1. josephin domain of ataxin-3, : 1 : 4 : 4 : 1 : 31 entities Detail
More details for 41 related entities
Interaction partners 1. josephin domain of ataxin-3, : 30 interactors Detail
More details for 30 interactors identified by 14 citations
Experiments performed 10 experiments Detail
nullKeywords ataxin-3, josephin, SCA3, polyglutamine disease