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Backbone Chemical Shift Assignments for G88W121 fragment of Staphylococcal Nuclease
Authors
Feng, Y., Liu, D., Wang, J.
Assembly
G88W121
Entity
1. G88W121 (polymer, Thiol state: not present), 121 monomers, 13717.78 Da Detail

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRWLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT H


Formula weight
13717.78 Da
Source organism
Staphylococcus aureus
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 81.8 %, Completeness: 41.9 %, Completeness (bb): 68.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All41.9 % (614 of 1467)40.6 % (312 of 768)35.3 % (202 of 572)78.7 % (100 of 127)
Backbone68.4 % (483 of 706)80.5 % (190 of 236)54.6 % (194 of 355)86.1 % (99 of 115)
Sidechain25.3 % (221 of 874)22.9 % (122 of 532)29.7 % (98 of 330) 8.3 % (1 of 12)
Aromatic 1.8 % (2 of 110) 1.8 % (1 of 55) 0.0 % (0 of 54)100.0 % (1 of 1)
Methyl20.8 % (27 of 130)21.5 % (14 of 65)20.0 % (13 of 65)

1. G88W121

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRWLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT H

Sample

Temperature 298 (±0.2) K, pH 5.0 (±0.1)


#NameIsotope labelingTypeConcentration
1G88W121[U-95% 13C; U-95% 15N]1 mM
2deuterated acetate[U-99% 2H]50 mM
3DSS50 uM
4NaN30.02 %

LACS Plot; CA
Referencing offset: 0.05 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 0.05 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.0 ppm, Outliers: 1 Detail
Release date
2004-08-02
Citation
Native-like partially folded conformations and folding process revealed in the N-terminal large fragments of staphylococcal nuclease: a study by NMR spectroscopy
Feng, Y., Liu, D., Wang, J.
J. Mol. Biol. (2003), 330, 821-837, PubMed 12850150 , DOI 10.1016/s0022-2836(03)00660-0 ,
Entries sharing articles BMRB: 2 entries Detail
  BMRB: 6251 released on 2004-08-02
    Title Backbone Chemical Shift Assignments for V66W121 fragment of Staphylococcal Nuclease
  BMRB: 5536 released on 2003-12-07
    Title 1H, 13C, and 15N Chemical Shift Assignments for G88W110 fragment of Staphylococcal Nuclease
Related entities 1. G88W121, : 1 : 64 : 50 entities Detail
Experiments performed 5 experiments Detail