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Backbone Chemical Shift Assignments for V66W121 fragment of Staphylococcal Nuclease
Authors
Feng, Y., Liu, D., Wang, J.
Assembly
V66W121
Entity
1. V66W121 (polymer, Thiol state: not present), 121 monomers, 13675.70 Da Detail

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMWENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT H


Formula weight
13675.7 Da
Source organism
Staphylococcus aureus
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 64.5 %, Completeness: 27.0 %, Completeness (bb): 48.7 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All27.0 % (395 of 1462)21.7 % (166 of 766)26.9 % (153 of 569)59.8 % (76 of 127)
Backbone48.7 % (343 of 705)51.3 % (121 of 236)41.2 % (146 of 354)66.1 % (76 of 115)
Sidechain14.0 % (122 of 869) 8.7 % (46 of 530)23.2 % (76 of 327) 0.0 % (0 of 12)
Aromatic 0.0 % (0 of 110) 0.0 % (0 of 55) 0.0 % (0 of 54) 0.0 % (0 of 1)
Methyl10.3 % (13 of 126) 6.3 % (4 of 63)14.3 % (9 of 63)

1. V66W121

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMWENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT H

Sample

Temperature 298 (±0.2) K, pH 5.0 (±0.1)


#NameIsotope labelingTypeConcentration
1V66W121[U-95% 13C; U-95% 15N]1 mM
2deuterated acetate[U-99% 2H]50 mM
3DSS50 uM
4NaN30.02 %

Chem. Shift Complete2
Sequence coverage: 28.1 %, Completeness: 15.9 %, Completeness (bb): 29.1 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All15.9 % (465 of 2924)13.1 % (200 of 1532)13.6 % (155 of 1138)43.3 % (110 of 254)
Backbone29.1 % (410 of 1410)32.2 % (152 of 472)20.9 % (148 of 708)47.8 % (110 of 230)
Sidechain 7.2 % (126 of 1738) 4.6 % (49 of 1060)11.8 % (77 of 654) 0.0 % (0 of 24)
Aromatic 0.0 % (0 of 220) 0.0 % (0 of 110) 0.0 % (0 of 108) 0.0 % (0 of 2)
Methyl 5.2 % (13 of 252) 3.2 % (4 of 126) 7.1 % (9 of 126)

1. V66W121

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMWENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT H

Sample

Temperature 298 (±0.2) K, pH 5.0 (±0.1)


#NameIsotope labelingTypeConcentration
1V66W121[U-95% 13C; U-95% 15N]1 mM
2deuterated acetate[U-99% 2H]50 mM
3DSS50 uM
4NaN30.02 %

Release date
2004-08-02
Citation
Native-like partially folded conformations and folding process revealed in the N-terminal large fragments of staphylococcal nuclease: a study by NMR spectroscopy
Feng, Y., Liu, D., Wang, J.
J. Mol. Biol. (2003), 330, 821-837, PubMed 12850150 , DOI 10.1016/s0022-2836(03)00660-0 ,
Entries sharing articles BMRB: 2 entries Detail
  BMRB: 6250 released on 2004-08-02
    Title Backbone Chemical Shift Assignments for G88W121 fragment of Staphylococcal Nuclease
  BMRB: 5536 released on 2003-12-07
    Title 1H, 13C, and 15N Chemical Shift Assignments for G88W110 fragment of Staphylococcal Nuclease
Related entities 1. V66W121, : 1 : 65 : 49 entities Detail