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Found 1 Document (1.665 seconds)

BMRB: 6277

4DDI

NMR solution structure of the ubiquitin conjugating enzyme UbcH5B

Authors

Houben, K., Dominguez, C., van Schaik, F.M.A., Timmers, M.H.T., Bonvin, A.M.J.J., Boelens, R.

Assembly

ubiquitin conjugatin enzyme 5B

Entity

1. ubiquitin conjugatin enzyme 5B (polymer, Thiol state: all free), 147 monomers, 16735.06 Da Detail

MALKRIHKEL NDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV PEIARIYKTD REKYNRIARE WTQKYAM

Formula weight

16735.06 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

ARIA

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 82.9 %, Completeness (bb): 95.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.9 % (1452 of 1752)	84.8 % (777 of 916)	76.9 % (530 of 689)	98.6 % (145 of 147)
Backbone	95.7 % (815 of 852)	94.7 % (270 of 285)	95.2 % (414 of 435)	99.2 % (131 of 132)
Sidechain	74.4 % (775 of 1041)	80.3 % (507 of 631)	64.3 % (254 of 395)	93.3 % (14 of 15)
Aromatic	41.2 % (66 of 160)	78.8 % (63 of 80)	0.0 % (0 of 77)	100.0 % (3 of 3)
Methyl	86.2 % (131 of 152)	98.7 % (75 of 76)	73.7 % (56 of 76)

1. UbcH5B

MALKRIHKEL NDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV PEIARIYKTD REKYNRIARE WTQKYAM

Show sample condition

Sample

Temperature 300 (±0) K, pH 7 (±0)

#	Name	Isotope labeling	Concentration
1	UbcH5B	[U-15N; U-13C]	0.3 mM
2	KCl		150 mM
3	Kpi		20 mM
4	ZnCl2		0.01 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type
¹³C	UbcH5B	aliphatic carbons	41.3 ppm	external	indirect
¹H	UbcH5B	protons	4.77 ppm	external	indirect
¹⁵N	UbcH5B	nitrogen	117.5 ppm	external	indirect

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type
¹³C	UbcH5B	aliphatic carbons	41.3 ppm	external	indirect
¹H	UbcH5B	protons	4.77 ppm	external	indirect
¹⁵N	UbcH5B	nitrogen	117.5 ppm	external	indirect

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type
¹³C	UbcH5B	aliphatic carbons	41.3 ppm	external	indirect
¹H	UbcH5B	protons	4.77 ppm	external	indirect
¹⁵N	UbcH5B	nitrogen	117.5 ppm	external	indirect

Referencing offset: -0.17 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type
¹³C	UbcH5B	aliphatic carbons	41.3 ppm	external	indirect
¹H	UbcH5B	protons	4.77 ppm	external	indirect
¹⁵N	UbcH5B	nitrogen	117.5 ppm	external	indirect

Referencing offset: 0.17 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 1W4U, Strand ID: A Detail

Release date

2005-02-20

Citation

Solution structure of the ubiquitin-conjugating enzyme UbcH5B
Houben, K., Dominguez, C., van Schaik, F.M.A., Timmers, M.H.T., Bonvin, A.M.J.J., Boelens, R.
J. Mol. Biol. (2004), 344, 513-526, PubMed 15522302 , DOI 10.1016/j.jmb.2004.09.054 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P62837 released on 2004-08-16
Title UB2D2_HUMAN Entity Ubiquitin-conjugating enzyme E2 D2

Related entities 1. ubiquitin conjugatin enzyme 5B, : 1 : 19 : 9 : 39 : 230 entities Detail

Interaction partners 1. ubiquitin conjugatin enzyme 5B, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 6 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr6277_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr6277_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts	Warning	1563		98.6 (chain: 1, length: 147)
1	Chemical shift references	chemical_shift_reference	Warning	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 147, Sequence coverage: 98.6%
- Total number of assignments
  - ¹³C chemical shifts: 519
  - ¹H chemical shifts: 900
  - ¹⁵N chemical shifts: 144
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	294	274	93.2
¹H chemical shifts	285	267	93.7
¹⁵N chemical shifts	132	130	98.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	689	519	75.3
¹H chemical shifts	916	752	82.1
¹⁵N chemical shifts	155	144	92.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	395	245	62.0
¹H chemical shifts	631	485	76.9
¹⁵N chemical shifts	23	14	60.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	77	0	0.0
¹H chemical shifts	80	59	73.8
¹⁵N chemical shifts	3	3	100.0

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Found 1 Document (1.665 seconds)

BMRB: 6277

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. ubiquitin conjugatin enzyme 5B, : 1 : 19 : 9 : 39 : 230 entities Detail

Interaction partners 1. ubiquitin conjugatin enzyme 5B, : 1 interactors Detail

Experiments performed 6 experiments Detail

Instrument

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Chemical shift validation 3 contents Detail