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BMRB: 6283

1WNJ

1H and 15N Chemical Shift Assignments for CLP

Authors

Liepinsh, E., Rakonjac, M., Boissonneault, V., Provost, P., Samuelsson, B., Radmark, O., Otting, G.

Assembly

coactosin like protein

Entity

1. coactosin like protein (polymer, Thiol state: all free), 145 monomers, 16271.17 Da Detail

GIRMATKIDK EACRAAYNLV RDDGSAVIWV TFKYDGSTIV PGEQGAEYQH FIQQCTDDVR LFAFVRFTTG DAMSKRSKFA LITWIGENVS GLQRAKTGTD KTLVKEVVQN FAKEFVISDR KELEEDFIKS ELKKAGGANY DAQTE

Formula weight

16271.17 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 97.3 %, Completeness (bb): 98.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	97.3 % (1003 of 1031)	97.1 % (849 of 874)	98.1 % (154 of 157)
Backbone	98.2 % (436 of 444)	98.3 % (295 of 300)	97.9 % (141 of 144)
Sidechain	96.6 % (567 of 587)	96.5 % (554 of 574)	100.0 % (13 of 13)
Aromatic	100.0 % (77 of 77)	100.0 % (75 of 75)	100.0 % (2 of 2)
Methyl	98.7 % (78 of 79)	98.7 % (78 of 79)

1. coactosin like protein

GIRMATKIDK EACRAAYNLV RDDGSAVIWV TFKYDGSTIV PGEQGAEYQH FIQQCTDDVR LFAFVRFTTG DAMSKRSKFA LITWIGENVS GLQRAKTGTD KTLVKEVVQN FAKEFVISDR KELEEDFIKS ELKKAGGANY DAQTE

Show sample condition

Sample

Temperature 298 (±1) K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
1	coactosin like protein	[U-90% 15N]	0.15 mM
2	Tris buffer		8 mM
3	DTT		1 mM
4	NaCl		50 mM
5	D2O		5 %

Release date

2004-12-14

Citation

Letter to the Editor: NMR Structure of Human Coactosin-Like Protein
Liepinsh, E., Rakonjac, M., Boissonneault, V., Provost, P., Samuelsson, B., Radmark, O., Otting, G.
J. Biomol. NMR (2004), 30, 353-356, PubMed , DOI:

Related entities 1. coactosin like protein, : 1 : 6 : 30 entities Detail

Interaction partners 1. coactosin like protein, : 7 interactors Detail

More details for 7 interactors identified by 4 citations

Experiments performed 6 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr6283_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr6283_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts	Warning	1150		99.3 (chain: 1, length: 145)
1	Chemical shift references	chemical_shift_reference	OK	2		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 145, Sequence coverage: 99.3%
- Total number of assignments
  - ¹H chemical shifts: 996
  - ¹⁵N chemical shifts: 154
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
31	THR	HG1	6.04
48	TYR	HH	9.14
55	CYS	HG	0.33

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	874	837	95.8
¹⁵N chemical shifts	165	154	93.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	300	295	98.3
¹⁵N chemical shifts	144	141	97.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	574	542	94.4
¹⁵N chemical shifts	21	13	61.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	81	78	96.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	75	75	100.0
¹⁵N chemical shifts	2	2	100.0

Keywords NMR, CLP

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Found 1 Document (1.327 seconds)

BMRB: 6283

Sample

Related entities 1. coactosin like protein, : 1 : 6 : 30 entities Detail

Interaction partners 1. coactosin like protein, : 7 interactors Detail

Experiments performed 6 experiments Detail

Instrument

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Chemical shift validation 3 contents Detail