1H and 15N assignment of SMRT DAD
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 80.3 % (432 of 538) | 77.8 % (360 of 463) | 96.0 % (72 of 75) |
Backbone | 96.5 % (195 of 202) | 96.3 % (131 of 136) | 97.0 % (64 of 66) |
Sidechain | 70.5 % (237 of 336) | 70.0 % (229 of 327) | 88.9 % (8 of 9) |
Aromatic | 57.1 % (28 of 49) | 58.3 % (28 of 48) | 0.0 % (0 of 1) |
Methyl | 92.3 % (24 of 26) | 92.3 % (24 of 26) |
1. SMRT deacetylase activation domain
NGLMADPMKV YKDRQVMNMW SEQEKETFRE KFMQHPKNFG LIASFLERKT VAECVLYYYL TKKNENYKTemperature 290 (±1) K, pH 6.8 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | SMRT deacetylase activation domain | 1.0 ~ 2.0 mM | ||
2 | NaCl | 50 mM | ||
3 | sodium phosphate | 50 mM |
Temperature 290 (±1) K, pH 6.8 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | SMRT deacetylase activation domain | [U-15N] | 1.0 ~ 2.0 mM | |
5 | NaCl | 50 mM | ||
6 | sodium phosphate | 50 mM |