BMRB: 6317

1H, 15N and 13C resonance assigment of the transcription factor CylR2 from Enterococcus faecalis

Authors

Rumpel, S., Vijayan, V., Zweckstetter, M.

Assembly

CylR2 dimer

Entity

1. CylR2 dimer (polymer, Thiol state: not present), 66 monomers, 7715.914 × 2 Da Detail

MIINNLKLIR EKKKISQSEL AALLEVSRQT INGIEKNKYN PSLQLALKIA YYLNTPLEDI FQWQPE

Total weight

15431.828 Da

Max. entity weight

7715.914 Da

Source organism

Enterococcus faecalis

Exptl. method

NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.4 %, Completeness (bb): 96.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.4 % (811 of 841)	96.0 % (427 of 445)	97.2 % (312 of 321)	96.0 % (72 of 75)
Backbone	96.2 % (375 of 390)	95.4 % (124 of 130)	97.0 % (191 of 197)	95.2 % (60 of 63)
Sidechain	96.7 % (499 of 516)	96.2 % (303 of 315)	97.4 % (184 of 189)	100.0 % (12 of 12)
Aromatic	100.0 % (46 of 46)	100.0 % (23 of 23)	100.0 % (22 of 22)	100.0 % (1 of 1)
Methyl	100.0 % (88 of 88)	100.0 % (44 of 44)	100.0 % (44 of 44)

1. cytolysin regulator R2

MIINNLKLIR EKKKISQSEL AALLEVSRQT INGIEKNKYN PSLQLALKIA YYLNTPLEDI FQWQPE

Show sample condition

Sample

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	cytolysin regulator R2	[U-95% 13C; U-90% 15N]		1.0 ~ 3.0 mM

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LACS Plot; CA

Referencing offset: 0.24 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.24 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.06 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 15 models in PDB: 2GZU, Strand ID: A, B Detail

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Release date

2004-12-21

Citation

Structure and DNA-binding properties of the cytolysin regulator CylR2 from Enterococcus faecalis
Rumpel, S., Razeto, A., Pillar, C.M., Vijayan, V., Taylor, A., Giller, K., Gilmore, M.S., Becker, S., Zweckstetter, M.
EMBO J. (2004), 23, 3632-3642, PubMed 15359276 , DOI 10.1038/sj.emboj.7600367 , Abstract

Related entities 1. CylR2 dimer, : 1 : 9 : 2 : 58 entities Detail

Experiments performed 1 experiments Detail