BMRB: 6320

1H, 13C, 15N chemical shift assignments for extended 3' internal stem-loop RNA from S. cerevisiae U6 snRNA

Authors

Sashital, D.G., Cornilescu, G., Butcher, S.E.

Assembly

U6 extended ISL

Entity

1. U6 extended ISL (polymer, Thiol state: not present), 32 monomers, 10303.10 Da Detail

GAGCAGUUCC CCUGCAUAAG GAUGAACCGU UC

Formula weight

10303.1 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.5 %, Completeness (bb): 93.2 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C	15N
All	88.5 % (453 of 512)	91.1 % (256 of 281)	87.5 % (189 of 216)	53.3 % (8 of 15)
Suger, PO4	93.2 % (328 of 352)	93.8 % (180 of 192)	92.5 % (148 of 160)
Nucleobase	78.1 % (125 of 160)	85.4 % (76 of 89)	73.2 % (41 of 56)	53.3 % (8 of 15)
Aromatic	81.7 % (116 of 142)	94.4 % (67 of 71)	73.2 % (41 of 56)	53.3 % (8 of 15)

1. U6 extended ISL

GAGCAGUUCC CCUGCAUAAG GAUGAACCGU UC

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Sample

Temperature 283 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	U6 extended ISL	[U-13C; U-15N]		1 mM

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Release date

2004-11-15

Citation

U2-U6 RNA folding reveals a group II intron-like domain and a four-helix junction
Sashital, D.G., Cornilescu, G., McManus, C.J., Brow, D.A., Butcher, S.E.
Nat. Struct. Mol. Biol. (2004), 11, 1237-1242, PubMed 15543154 , DOI 10.1038/nsmb863 , Abstract

Related entities 1. U6 extended ISL, : 1 : 2 entities Detail

Experiments performed 6 experiments Detail