BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	60.8 % (717 of 1179)	68.7 % (420 of 611)	42.6 % (197 of 462)	94.3 % (100 of 106)
Backbone	78.7 % (466 of 592)	90.8 % (187 of 206)	65.2 % (189 of 290)	93.8 % (90 of 96)
Sidechain	49.5 % (335 of 677)	57.5 % (233 of 405)	35.1 % (92 of 262)	100.0 % (10 of 10)
Aromatic	19.9 % (27 of 136)	38.2 % (26 of 68)	0.0 % (0 of 67)	100.0 % (1 of 1)
Methyl	51.1 % (48 of 94)	78.7 % (37 of 47)	23.4 % (11 of 47)

1. Delta25-PYP light state

LAFGAIQLDG DGNILQYNAA EGDITGRDPK QVIGKNFFKD VAPCTDSPEF YGKFKEGVAS GNLNTMFEYT FDYQMTPTKV KVHMKKALSG DSYWVFVKRV

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Sample #1

Temperature 293 (±0) K, pH 7 (±0)

#	Name	Isotope labeling	Type	Concentration
1	Delta25-PYP light state	[U-13C; U-15N]		1 mM
2	phosphate buffer			50 mM

Sample #2

Temperature 293 (±0) K, pH 7 (±0)

#	Name	Isotope labeling	Type	Concentration
3	Delta25-PYP light state	[U-15N]		1 mM
4	phosphate buffer			50 mM

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Calculated from 20 models in PDB: 1XFQ, Strand ID: A Detail

Release date

2005-07-24

Citation

The solution structure of a transient photoreceptor intermediate: Delta25 photoactive yellow protein
Bernard, C., Houben, K., Derix, N.M., Marks, D., van der Horst, M.A., Hellingwerf, K.J., Boelens, R., Kaptein, R., van Nuland, N.A.
Structure (2005), 13, 953-962, PubMed 16004868 , DOI 10.1016/j.str.2005.04.017 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 6321 released on 2005-07-24
Title Chemical Shift assignment of the Ground-State of Delta25-PYP

Related entities 1. delta25-PYP, : 1 : 45 : 17 : 34 : 12 entities Detail

Experiments performed 9 experiments Detail

1 1H15N HSQC

Instrument

#1 Model Bruker Avance (900 MHz)

#2 Model Bruker Avance (750 MHz)

#3 Model Bruker Avance (500 MHz)

Sample #1

Temperature 293 (±0) K, pH 7 (±0)

#	Name	Isotope labeling	Type	Concentration
1	Delta25-PYP light state	[U-13C; U-15N]		1 mM
2	phosphate buffer			50 mM

Sample #2

Temperature 293 (±0) K, pH 7 (±0)

#	Name	Isotope labeling	Type	Concentration
3	Delta25-PYP light state	[U-15N]		1 mM
4	phosphate buffer			50 mM

2 1H 2D NOESY

Instrument

#1 Model Bruker Avance (900 MHz)

#2 Model Bruker Avance (750 MHz)

#3 Model Bruker Avance (500 MHz)

Sample #1

Temperature 293 (±0) K, pH 7 (±0)

#	Name	Isotope labeling	Type	Concentration
1	Delta25-PYP light state	[U-13C; U-15N]		1 mM
2	phosphate buffer			50 mM

Sample #2

Temperature 293 (±0) K, pH 7 (±0)

#	Name	Isotope labeling	Type	Concentration
3	Delta25-PYP light state	[U-15N]		1 mM
4	phosphate buffer			50 mM

3 1H 2D TOCSY

Instrument

#1 Model Bruker Avance (900 MHz)

#2 Model Bruker Avance (750 MHz)

#3 Model Bruker Avance (500 MHz)

Sample #1

Temperature 293 (±0) K, pH 7 (±0)

#	Name	Isotope labeling	Type	Concentration
1	Delta25-PYP light state	[U-13C; U-15N]		1 mM
2	phosphate buffer			50 mM

Sample #2

Temperature 293 (±0) K, pH 7 (±0)

#	Name	Isotope labeling	Type	Concentration
3	Delta25-PYP light state	[U-15N]		1 mM
4	phosphate buffer			50 mM

4 1H 15N NOESY HSQC

Instrument

#1 Model Bruker Avance (900 MHz)

#2 Model Bruker Avance (750 MHz)

#3 Model Bruker Avance (500 MHz)

Sample #1

Temperature 293 (±0) K, pH 7 (±0)

#	Name	Isotope labeling	Type	Concentration
1	Delta25-PYP light state	[U-13C; U-15N]		1 mM
2	phosphate buffer			50 mM

Sample #2

Temperature 293 (±0) K, pH 7 (±0)

#	Name	Isotope labeling	Type	Concentration
3	Delta25-PYP light state	[U-15N]		1 mM
4	phosphate buffer			50 mM

5 1H 15N TOCSY HSQC

Instrument

#1 Model Bruker Avance (900 MHz)

#2 Model Bruker Avance (750 MHz)

#3 Model Bruker Avance (500 MHz)

Sample #1

Temperature 293 (±0) K, pH 7 (±0)

#	Name	Isotope labeling	Type	Concentration
1	Delta25-PYP light state	[U-13C; U-15N]		1 mM
2	phosphate buffer			50 mM

Sample #2

Temperature 293 (±0) K, pH 7 (±0)

#	Name	Isotope labeling	Type	Concentration
3	Delta25-PYP light state	[U-15N]		1 mM
4	phosphate buffer			50 mM

6 HNCA

Instrument

#1 Model Bruker Avance (900 MHz)

#2 Model Bruker Avance (750 MHz)

#3 Model Bruker Avance (500 MHz)

Sample #1

Temperature 293 (±0) K, pH 7 (±0)

#	Name	Isotope labeling	Type	Concentration
1	Delta25-PYP light state	[U-13C; U-15N]		1 mM
2	phosphate buffer			50 mM

Sample #2

Temperature 293 (±0) K, pH 7 (±0)

#	Name	Isotope labeling	Type	Concentration
3	Delta25-PYP light state	[U-15N]		1 mM
4	phosphate buffer			50 mM

7 CBCA(CO)NH

Instrument

#1 Model Bruker Avance (900 MHz)

#2 Model Bruker Avance (750 MHz)

#3 Model Bruker Avance (500 MHz)

Sample #1

Temperature 293 (±0) K, pH 7 (±0)

#	Name	Isotope labeling	Type	Concentration
1	Delta25-PYP light state	[U-13C; U-15N]		1 mM
2	phosphate buffer			50 mM

Sample #2

Temperature 293 (±0) K, pH 7 (±0)

#	Name	Isotope labeling	Type	Concentration
3	Delta25-PYP light state	[U-15N]		1 mM
4	phosphate buffer			50 mM

8 HNCACB

Instrument

#1 Model Bruker Avance (900 MHz)

#2 Model Bruker Avance (750 MHz)

#3 Model Bruker Avance (500 MHz)

Sample #1

Temperature 293 (±0) K, pH 7 (±0)

#	Name	Isotope labeling	Type	Concentration
1	Delta25-PYP light state	[U-13C; U-15N]		1 mM
2	phosphate buffer			50 mM

Sample #2

Temperature 293 (±0) K, pH 7 (±0)

#	Name	Isotope labeling	Type	Concentration
3	Delta25-PYP light state	[U-15N]		1 mM
4	phosphate buffer			50 mM

9 13C Filtered 2D NOESY

Instrument

#1 Model Bruker Avance (900 MHz)

#2 Model Bruker Avance (750 MHz)

#3 Model Bruker Avance (500 MHz)

Sample #1

Temperature 293 (±0) K, pH 7 (±0)

#	Name	Isotope labeling	Type	Concentration
1	Delta25-PYP light state	[U-13C; U-15N]		1 mM
2	phosphate buffer			50 mM

Sample #2

Temperature 293 (±0) K, pH 7 (±0)

#	Name	Isotope labeling	Type	Concentration
3	Delta25-PYP light state	[U-15N]		1 mM
4	phosphate buffer			50 mM

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BMRB: 6322

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Entries sharing articles BMRB: 1 entries Detail

Related entities 1. delta25-PYP, : 1 : 45 : 17 : 34 : 12 entities Detail

Experiments performed 9 experiments Detail

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