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BMRB: 6331

2DCR

NMR assignment of the SH2 domain from the human feline sarcoma oncogene FES

Authors

Scott, A., Pantoja-Uceda, D., Koshiba, S., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Tanaka, A., Sugano, S., Yokoyama, S., Guntert, P.

Assembly

SH2 domain

Entity

1. SH2 domain (polymer, Thiol state: all free), 114 monomers, 12490.98 Da Detail

GSSGSSGEVQ KPLHEQLWYH GAIPRAEVAE LLVHSGDFLV RESQGKQEYV LSVLWDGLPR HFIIQSLDNL YRLEGEGFPS IPLLIDHLLS TQQPLTKKSG VVLHRAVPSG PSSG

Formula weight

12490.98 Da

Source organism

Exptl. method

Refine. method

automated NOESY assignment, torsion angle dynamics, energy minimization

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.6 %, Completeness: 93.6 %, Completeness (bb): 92.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.6 % (1235 of 1319)	94.2 % (645 of 685)	93.6 % (485 of 518)	90.5 % (105 of 116)
Backbone	92.2 % (616 of 668)	90.9 % (211 of 232)	93.9 % (310 of 330)	89.6 % (95 of 106)
Sidechain	94.8 % (714 of 753)	95.8 % (434 of 453)	93.1 % (270 of 290)	100.0 % (10 of 10)
Aromatic	90.2 % (92 of 102)	100.0 % (51 of 51)	79.6 % (39 of 49)	100.0 % (2 of 2)
Methyl	97.1 % (132 of 136)	95.6 % (65 of 68)	98.5 % (67 of 68)

1. SH2 domain from the human feline sarcoma oncogene FES

GSSGSSGEVQ KPLHEQLWYH GAIPRAEVAE LLVHSGDFLV RESQGKQEYV LSVLWDGLPR HFIIQSLDNL YRLEGEGFPS IPLLIDHLLS TQQPLTKKSG VVLHRAVPSG PSSG

Show sample condition

Sample

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	SH2 domain from the human feline sarcoma oncogene FES	[U-13C; U-15N]		1.2 mM

LACS Plot; CA

Referencing offset: 2.14 ppm, Outliers: 3 Detail

LACS Plot; CB

Referencing offset: 2.14 ppm, Outliers: 3 Detail

LACS Plot; HA

Referencing offset: 0.19 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: 2.66 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1WQU, Strand ID: A Detail

Release date

2005-01-06

Citation

Letter to the Editor: NMR assignment of the SH2 domain from the human feline sarcoma oncogene FES
Scott, A., Pantoja-Uceda, D., Koshiba, S., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Tanaka, A., Sugano, S., Yokoyama, S., Guntert, P.
J. Biomol. NMR (2004), 30, 463-464, DOI 10.1007/s10858-004-5432-z

Related entities 1. SH2 domain, : 1 : 1 : 7 : 123 entities Detail

Interaction partners 1. SH2 domain, : 24 interactors Detail

More details for 24 interactors identified by 15 citations

Experiments performed 15 experiments Detail

1 15N HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	SH2 domain from the human feline sarcoma oncogene FES	[U-13C; U-15N]		1.2 mM

2 15N NOESY-HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	SH2 domain from the human feline sarcoma oncogene FES	[U-13C; U-15N]		1.2 mM

3 15N TOCSY-HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	SH2 domain from the human feline sarcoma oncogene FES	[U-13C; U-15N]		1.2 mM

4 13C HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	SH2 domain from the human feline sarcoma oncogene FES	[U-13C; U-15N]		1.2 mM

5 13C NOESY-HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	SH2 domain from the human feline sarcoma oncogene FES	[U-13C; U-15N]		1.2 mM

6 HCCH-TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	SH2 domain from the human feline sarcoma oncogene FES	[U-13C; U-15N]		1.2 mM

7 HCCH-COSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	SH2 domain from the human feline sarcoma oncogene FES	[U-13C; U-15N]		1.2 mM

8 HNCO

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	SH2 domain from the human feline sarcoma oncogene FES	[U-13C; U-15N]		1.2 mM

9 HN(CO)CA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	SH2 domain from the human feline sarcoma oncogene FES	[U-13C; U-15N]		1.2 mM

10 HNCA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	SH2 domain from the human feline sarcoma oncogene FES	[U-13C; U-15N]		1.2 mM

11 CBCA(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	SH2 domain from the human feline sarcoma oncogene FES	[U-13C; U-15N]		1.2 mM

12 CBCANH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	SH2 domain from the human feline sarcoma oncogene FES	[U-13C; U-15N]		1.2 mM

13 HBHA(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	SH2 domain from the human feline sarcoma oncogene FES	[U-13C; U-15N]		1.2 mM

14 (H)CC(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	SH2 domain from the human feline sarcoma oncogene FES	[U-13C; U-15N]		1.2 mM

15 H(CCCO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	SH2 domain from the human feline sarcoma oncogene FES	[U-13C; U-15N]		1.2 mM

nullKeywords Assignment note