BMRBj - BMREntryMaster

Found 1 Document (1.694 seconds)

BMRB: 6342

1XOX

Backbone 1H, 13C, and 15N Chemical Shift Assignments for human survivin

Authors

Olejniczak, E.T., Nettesheim, D., Sun, C., Liu, Z.

Assembly

survivin dimer

Entity

1. survivin dimer (polymer, Thiol state: all free), 117 monomers, 13458.17 × 2 Da Detail

MGAPTLPPAW QPFLKDHRIS TFKNWPFLEG CACTPERMAE AGFIHCPTEN EPDLAQCFFC FKELEGWEPD DDPIEEHKKH SSGCAFLSVK KQFEELTLGE FLKLDRERAK NKIAKET

Total weight

26916.34 Da

Max. entity weight

13458.17 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 82.3 %, Completeness (bb): 88.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.3 % (1160 of 1409)	80.9 % (600 of 742)	82.9 % (457 of 551)	88.8 % (103 of 116)
Backbone	88.4 % (603 of 682)	93.5 % (215 of 230)	83.8 % (289 of 345)	92.5 % (99 of 107)
Sidechain	78.8 % (660 of 838)	75.2 % (385 of 512)	85.5 % (271 of 317)	44.4 % (4 of 9)
Aromatic	84.9 % (129 of 152)	88.2 % (67 of 76)	80.8 % (59 of 73)	100.0 % (3 of 3)
Methyl	97.8 % (88 of 90)	97.8 % (44 of 45)	97.8 % (44 of 45)

1. survivin

MGAPTLPPAW QPFLKDHRIS TFKNWPFLEG CACTPERMAE AGFIHCPTEN EPDLAQCFFC FKELEGWEPD DDPIEEHKKH SSGCAFLSVK KQFEELTLGE FLKLDRERAK NKIAKET

Show sample condition

Sample

Temperature 298 K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	survivin	[U-13C; U-15N; U-75% 2H]		0.7 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -1.81 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 1XOX, Strand ID: A, B Detail

Release date

2005-03-15

Citation

Solution structure of human survivin and its binding interface with Smac/Diablo
Sun, C., Nettesheim, D., Liu, Z., Olejniczak, E.T.
Biochemistry (2005), 44, 11-17, PubMed 15628841 , DOI 10.1021/bi0485171 , Abstract

Related entities 1. survivin dimer, : 1 : 23 : 1 : 6 : 23 entities Detail

Experiments performed 7 experiments Detail

1 HNCA

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	survivin	[U-13C; U-15N; U-75% 2H]		0.7 mM

Sample #2

Temperature 298 K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	survivin	[U-13C; U-15N]		1.0 mM

2 HN(CO)CA

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	survivin	[U-13C; U-15N; U-75% 2H]		0.7 mM

Sample #2

Temperature 298 K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	survivin	[U-13C; U-15N]		1.0 mM

3 HN(CA)CB

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	survivin	[U-13C; U-15N; U-75% 2H]		0.7 mM

Sample #2

Temperature 298 K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	survivin	[U-13C; U-15N]		1.0 mM

4 HN(COCA)CB

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	survivin	[U-13C; U-15N; U-75% 2H]		0.7 mM

Sample #2

Temperature 298 K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	survivin	[U-13C; U-15N]		1.0 mM

5 HNCO

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	survivin	[U-13C; U-15N; U-75% 2H]		0.7 mM

Sample #2

Temperature 298 K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	survivin	[U-13C; U-15N]		1.0 mM

6 HN(CA)CO

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	survivin	[U-13C; U-15N; U-75% 2H]		0.7 mM

Sample #2

Temperature 298 K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	survivin	[U-13C; U-15N]		1.0 mM

7 13C, 15N edit/seperated Noesy

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	survivin	[U-13C; U-15N; U-75% 2H]		0.7 mM

Sample #2

Temperature 298 K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	survivin	[U-13C; U-15N]		1.0 mM

nullKeywords NMR, bir domain

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Found 1 Document (1.694 seconds)

BMRB: 6342

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. survivin dimer, : 1 : 23 : 1 : 6 : 23 entities Detail

Experiments performed 7 experiments Detail

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