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1H, 15N and 13C Resonance Assignments and Secondary Structure Determination Reveal that the Minimal Rac1 GTPase Binding Domain of Plexin-B1 Has a Ubiquitin Fold
Authors
Tong, Y., Buck, M.
Assembly
h. plexin-B1 RBD-W1830F
Entity
1. h. plexin-B1 RBD-W1830F (polymer, Thiol state: all free), 122 monomers, 13441.26 Da Detail

KKDVEYRPLT LNALLAVGPG AGEAQGVPVK VLDCDTISQA KEKMLDQLYK GVPLTQRPDP RTLDVEWRSG VAGHLILSDE DVTSEVQGLF RRLNTLQHYK VPDGATVALV PCLTKHVLRE NQ


Formula weight
13441.26 Da
Source organism
Homo sapiens
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.4 %, Completeness: 91.7 %, Completeness (bb): 95.8 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All91.7 % (1292 of 1409)92.0 % (671 of 729)91.6 % (502 of 548)90.2 % (119 of 132)
Backbone95.8 % (677 of 707)95.3 % (225 of 236)96.4 % (344 of 357)94.7 % (108 of 114)
Sidechain88.7 % (723 of 815)90.5 % (446 of 493)87.5 % (266 of 304)61.1 % (11 of 18)
Aromatic19.0 % (11 of 58)31.0 % (9 of 29) 3.6 % (1 of 28)100.0 % (1 of 1)
Methyl99.4 % (163 of 164)100.0 % (82 of 82)98.8 % (81 of 82)

1. plexin-B1 RBD

KKDVEYRPLT LNALLAVGPG AGEAQGVPVK VLDCDTISQA KEKMLDQLYK GVPLTQRPDP RTLDVEWRSG VAGHLILSDE DVTSEVQGLF RRLNTLQHYK VPDGATVALV PCLTKHVLRE NQ

Sample

Temperature 298 (±0.1) K, pH 6.8 (±0.2)


#NameIsotope labelingTypeConcentration
1plexin-B1 RBD[U-13C; U-15N]1.2 mM

LACS Plot; CA
Referencing offset: 0.59 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 0.59 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.11 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.37 ppm, Outliers: 1 Detail
Release date
2005-07-24
Citation 1
1H, 15N and 13C Resonance assignments and secondary structure determination reveal that the minimal Rac1 GTPase binding domain of plexin-B1 has a ubiquitin fold
Tong, Y., Buck, M.
J. Biomol. NMR (2005), 31, 369-370, PubMed 15929008 , DOI 10.1007/s10858-005-0943-9 ,
Citation 2
The semaphorin receptor plexin-B1 specifically interacts with active Rac in a ligand-dependent manner
Vikis, H.G., Li, W., He, Z., Guan, K.L.
Proc. Natl. Acad. Sci. U. S. A. (2000), 97, 12457-12462, PubMed 11035813 , DOI 10.1073/pnas.220421797 ,
Related entities 1. h. plexin-B1 RBD-W1830F, : 1 : 4 : 34 entities Detail
Interaction partners 1. h. plexin-B1 RBD-W1830F, : 19 interactors Detail
Experiments performed 14 experiments Detail